Response of Osmotic Regulation Substance Content and Protective Enzyme Activities to Shading in Leaves of Different Rice Genotypes

LIU Li1, WANG Li1, DENG Fei1, HUANG Yun1, LIU Dai-yin2, REN Wan-jun1, YANG Wen-yu1

doi:10.1016/S1672-6308(13)60137-7

2013 , Vol. 20 >Issue 4: 276 - 283

DOI: https://doi.org/10.1016/S1672-6308(13)60137-7

Research Paper

Response of Osmotic Regulation Substance Content and Protective Enzyme Activities to Shading in Leaves of Different Rice Genotypes

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1College of Agronomy, Sichuan Agricultural University / Key Laboratory of Crop Physiology, Ecology and Cultivation in Southwest China, Wenjiang 611130, China; 2Sichuan General Popularization Centre of Agricultural Technique, Chengdu 610041, China

Online published: 2013-05-20

Supported by

This research was supported by the 11th 5-year Major Project of National Science and Technology Support Plan (Grant No. 2011BAD16B05); Youth Foundation Project of Sichuan Agricultural University in China and Outstanding Master’s Thesis Foundation Project of Sichuan Agricultural University in China.

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Abstract

Effects of shading at the flowering and grain filling stages on osmotic regulation substance content and protective enzyme activities in the leaves of different rice genotypes (e.g., IIyou 498, Gangyou 188, Dexiang 4103, Gangyou 527 and Chuanxiang 9838) were investigated. The results showed that the malondialdehyde content of Gangyou 188 significantly increased under shading, whereas those of IIyou 498, Dexiang 4103, Gangyou 527 and Chuanxiang 9838 showed no significant differences compared to the control. The soluble sugar content significantly decreased in IIyou 498, Gangyou 188, Dexiang 4103 and Chuanxiang 9838, whereas it did not significantly increase in Gangyou 527 under the weak light conditions (shading). Moreover, the soluble protein content in the leaves of different genotypes tended to decrease under shading. Further, the protective enzyme activities in the leaves varied in different rice genotypes under the shading treatment. Based on the osmotic regulation substance content and the protective enzyme activities in the leaves of different rice genotypes, it was concluded that Gangyou 188 and Gangyou 527 had strong abilities to adapt to the low light conditions. In addition, the mechanism of damage to rice leaves in different genotypes under shading was discussed.

Key words： malondialdehyde content; osmotic regulation substance content; protective enzyme; rice genotype; shading

Cite this article

LIU Li1, WANG Li1, DENG Fei1, HUANG Yun1, LIU Dai-yin2, REN Wan-jun1, YANG Wen-yu1 . Response of Osmotic Regulation Substance Content and Protective Enzyme Activities to Shading in Leaves of Different Rice Genotypes[J]. Rice Science, 2013 , 20(4) : 276 -283 . DOI: 10.1016/S1672-6308(13)60137-7

References

Boardman N K. 1977. Comparative photosynthesis of sun and shading plants. Annu Rev Plant Physiol, 28: 355–377.
Cai K Z, Wu X Z, Luo S M. 2008. Effects of water stress on osmolytes at different growth stages in rice leaves and roots. Chin J Plant Ecol, 32(2): 491–500. (in Chinese with English abstract)
Dhinsa R S, Dhindsa P P, Thorpe T A. 1981. Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxidation dismutase and catalase. J Exp Bot, 32: 93.
Ding S B, Zhu B Y, Wu D Y, Zhang L. 2004. Effect of temperature and light on senescence of flag leaf and grain-filling after rice heading. J South China Norm Univ: Nat Sci, (1): 117–128. (in Chinese with English abstract)
Ding S S, Su P X. 2010. Effects of tree shading on maize crop within a poplar-maize compound system in Hexi Corridor oasis, Northwestern China. Agric Syst, 80: 117–129.
Dong Y C, Liu Y Q. 2009. Soil water influences on protective enzymes and osmolytes of Urtica dioica and their correlations with leaf photosynthesis and biomass. Acta Ecol Sin, 29(6): 2845–2851. (in Chinese with English abstract)
Guan X, Chen W F, Yin H, Li Z X, Lu X L. 2003. Comparison on the protective enzyme activities and lipid peroxidation after full heading stage among rice varieties developed in different years. J Shenyang Agric Univ, 34(5): 351–354. (in Chinese with English abstract)
Hou Y P, Chen N L, Kang E X, Zhang Y X. 2009. Effects of low temperature and low light stress on osmotic adjustment of squash seedlings. China Cucurbits Vegetable, (1): 8–12. (in Chinese with English abstract)
Huang J, Guo S R, Jiang F L, Chen L, Wu Z. 2008. Effects of shading treatment and regular light recovery on growth and reactive oxygen metabolism of pak-choi. Acta Hort Sin, 35(5): 753–756. (in Chinese with English abstract)
Jia C H, Guo T J, Wang G, Xu D, Tong X, Xu Z S. 2010. Effects of different shading treatments on chlorophyll and protective enzyme activities of Sabina davurica. J Jilin Agric Univ, 32(3): 264–267. (in Chinese with English abstract)
Kumudini S, Omielan J, Hume D J. 2008. Soybean genetic improvement in yield and the effect of late-season shading and nitrogen source and supply. Agron J, 100(2): 400–405.
Lazaro L, Abbate P E, Cogliatti D H, Andrade F H. 2010. Relationship between yield, growth and spike weight in wheat under phosphorus de?ciency and shading. J Agric Sci, 148: 83–93.
Li H S. 2002. Experiment Principle and Technology of Plant Physiology. Beijing: Higher Education Press. (in Chinese)
Li T, Ohsugi R, Yamagishi T, Sasaki H. 2005. Effects of weak light on rice starch accumulation and starch synthesis enzyme activities at grain filling stage. Chin J Rice Sci, 19(6): 545–550. (in Chinese with English abstract)
Liu Y Y, Teng Z H, Wang S G, He G H. 2008. Effects of high temperature stress on soluble sugar and membrane protective enzyme in rice. J Southwest Univ: Nat Sci, 30(2): 59–63. (in Chinese with English abstract)
Longstreth D J. 1980. Nutrient in fluence on leaf photosynthesis effects on nitrogen phosphorus and potassium for Gossypium hirsutrum. Plant Physiol, 65(3): 541–543.
Murty K S, Sahu G. 1987. Impact of low-light stress on growth and yield of rice. In: Weather and Rice. Metro Manila, Philippimes: International Rice Research Institute: 93–101.
Okada K, Katoh S. 1998. Two long-term effects of light that control the stability of proteins related to photosynthesis during senescence of rice leaves. Plant Cell Physiol, 39(4): 394–404.
Pan X H, Liu S Y, Li F, Li M Y. 2003. Effect of low-phosphorus stress on membrane lipid peroxidation and protective enzyme activities in rice leaves of different cultivars. Chin J Rice Sci, 17(1): 57–60. (in Chinese with English abstract)
Peng G Z, Yang X J. 2001. A study on the rice climatic harvest patterns and yield model in Sichuan Basin. Agric Meteor, 22(4): 6–10. (in Chinese with English abstract)
Ren W J, Yang W Y, Xu J W, Fan G Q, Wang LY, Guan H. 2002. Effects of weak light on the rice leaf characteristics in different genotypes after initial heading stage. J Sichuan Agric Univ, 20(3): 205–208. (in Chinese with English abstract)
Ren W J, Yang W Y, Zhang G Z, Zhu X, Fan G Q, Xu J W. 2003. Effects of low-light stress on nitrogen accumulation, distribution and grains protein content of indica hybrid. Plant Nutr Fert Sci, 9(3): 288–293. (in Chinese with English abstract)
Shi Y W, Wang Y L, Li W B, Gao S M, Li X. 2007. Effects of water stress on soluble protein, soluble sugar and proline content in Tamarix hispida. J Xinjiang Agric Univ, 30(2): 5–8. (in Chinese with English abstract)
Kato T. 1986. Effects of the shading and rachis-branch clipping on the grain-filling process of rice cultivars differing in the grain size. Jpn J Crop Sci, 55(2): 252–260.
Wang S H, Hao C L, Zhang Z X. 1998. The study and advance in the shade effect of plant. J Shandong Agric Univ, 29(1): 132–136. (in Chinese with English abstract)
Xia X L, Zheng C X, Yin W L. 2000. Effect of drought stress on the peroxidation of membrane lipids, the composition of membrane fatty acids and ethylene evolution of needles of Pinus sylvestris var. mongolica Litv. Sci Silv Sin, 36(3): 8–12. (in Chinese with English abstract)
Xiong Q E. 2003. Laboratory Experiments in Plant Physiology. Chengdu: Sichuan Science and Technology Publishing House. (in Chinese)
Xu L Z, Cai J, Jiang Z M, Peng X B, Su X F, Zhang S X. 2008. Effects of water stress on osmotic adjustment and activity of protect enzymes in the leaves of three sorts of seedlings. J Northwest Forest Univ, 23(2): 12–16. (in Chinese with English abstract)
Yang Y, Tian M J, Jiang X M, Wei W, Huang Y Y, Zeng Y H, Zhao R. 2010. Effects of shades on activities in antioxidant enzymes and biochemistry substances content of male and female Ginkgo biloba L. leaf during the rejuvenation period. Chin J Spectrosc Lab, 27(3): 918–921. (in Chinese with English abstract)
Zhu W Q, Wu L H, Tao Q N. 2003. Effects of nitrogen on soluble osmotica in rice plants in response to drought stress. J Zhejiang Univ: Agric Sci, 29(5): 479–484. (in Chinese with English abstract)
Zhou Y, Dong Y Z, Zhu Y, Wu W, Tan Y. 2010. Response of soil moisture stress on the membrane protective system of 5 different genotype of iris species in Xinjiang. Northern Hort, 11: 116–118. (in Chinese with English abstract)

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