Molecular Variation and Application from Aerospace Mutagenesis in Upland Rice Huhan 3 and Huhan 7

YU  Shun-wu, LUO  Hua-cheng, LI  Jia-jia, YU  Xin-qiao

doi:10.1016/S1672-6308(13)60144-4

Rice Science >

2013 , Vol. 20 >Issue 4: 249 - 258

DOI: https://doi.org/10.1016/S1672-6308(13)60144-4

Research Paper

Molecular Variation and Application from Aerospace Mutagenesis in Upland Rice Huhan 3 and Huhan 7

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Shanghai Agrobiological Gene Center, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; #These authors contributed equally to this study

Online published: 2013-05-20

Supported by

This study was supported by the National High-Technology Research and Development Program of China (Grant No. 2010AA101805), National Key Technology R&D Program of China (Grant No. 2013ZX08001-003) and Shanghai Agricultural Science and Technology Key Projects, China (Grant No. 2009-6-4-2).

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Abstract

To further improve upland rice varieties Huhan 3 and Huhan 7, seed samples were sent to outer space with two recoverable spaceships for approximately 1 and 5 d and were propagated for 7 and 5 generations, respectively. Phenotypic analysis revealed that the morphological traits and the protein and amylose contents of grains changed. Characterization of genomic mutations by the gene-associated simple sequence repeat (SSR) and insertion-delete (InDel) markers indicated that the mutation pattern was very complex. Most of the mutations occurred at the 3′- or 5′-end of the fragments in the simple sequence repeat fragment. Reverse transcription-polymerase chain reaction (RT-PCR) assay showed that mutations in those parts of the SSR affected their gene expression, indicating that gene associated markers would be helpful to isolate functional genes. Field survey for breeding also revealed that more lines with high yield, high quality and drought-tolerance could be selected through aerospace breeding. The results indicate that aerospace mutagenesis resulted in molecular variation, as well as physiological and morphological changes for rice breeding.

Key words： spaceflight; molecular marker; mutation; upland rice; gene expression

Cite this article

YU Shun-wu, LUO Hua-cheng, LI Jia-jia, YU Xin-qiao . Molecular Variation and Application from Aerospace Mutagenesis in Upland Rice Huhan 3 and Huhan 7[J]. Rice Science, 2013 , 20(4) : 249 -258 . DOI: 10.1016/S1672-6308(13)60144-4

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