Short Communication

Development of Novel Cytoplasmic Male Sterile Source from Dongxiang Wild Rice (Oryza rufipogon)

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  • Rice Research Institute, Jiangxi Academy of Agricultural Sciences / Jiangxi Provincial Key Laboratory for Physiology and Genetics of Rice, Nanchang 330200, China

Online published: 2013-07-30

Supported by

This research was supported by the Key Program for Science and Technology of Jiangxi Province, China (Grant No. 20114ABF03101) and the Program of Advantage Group for Innovation of Science and Technology of Jiangxi Province, China (Grant No. 20115BCB29019).

Abstract

This study was conducted to develop and characterize a novel cytoplasmic male sterile (CMS) source which was identified from Dongxiang wild rice (Oryza rufipogon) by crossing Dongxiang wild rice as female with Zhongzao 35, an indica inbred variety, as male and continuous backcrossing with Zhongzao 35. Observation under optical microscope manifested that this novel CMS belonged to typical abortion type with less pollen compared with wild abortive type cytoplasm (CMS-WA). Sequential planting showed that this novel CMS has complete and stable male sterility. Testcross experiment showed that all the 24 tested materials including maintainer and restorer lines of CMS-WA and Honglian type cytoplasm (CMS-HL) and other indica inbred varieties are the maintainers with complete maintaining ability, suggesting that this novel CMS has fertility restoration totally different from CMS-WA and CMS-HL and belongs to a novel type of CMS. So far, we only discovered a unique fertility restoration source for this novel CMS. Inheritance analysis showed that the fertility restoration of this CMS was governed by three pairs of independent dominant genes. Prospect for application of this novel CMS system in hybrid rice breeding was also discussed.

Cite this article

SHEN Xian-hua, YAN Song, HUANG Ren-liang, ZHU Shan, XIONG Hong-liang, SHEN Lin-jun . Development of Novel Cytoplasmic Male Sterile Source from Dongxiang Wild Rice (Oryza rufipogon)[J]. Rice Science, 2013 , 20(5) : 379 -382 . DOI: 10.1016/S1672-6308(13)60145-6

References

Chen D Z, Xiao Y Q, Zhao S X, Xiong H J. 1995. Studies on the restoring souce for CMS lines with the cytoplasm of Jiangxi Dongxiang wild rice. Hybrid Rice, 6: 4–6. (in Chinese)
Dalmacio R, Brar D S, Ishii T, Sitch L A, Virmani S S, Khush G S. 1995. Identification and transfer of a new cytoplasmic male sterility source from Oryza perennis into indica rice (O. sativa). Euphytica, 82: 221–225.
Fujii S, Toriyama K. 2005. Molecular mapping of the fertility restorer gene for ms-CW-type cytoplasmic male sterility of rice. Theor Appl Genet, 111(4): 696–701.
Hooker A L. 1974. Cytoplasmic susceptibility in plant disease. Annu Rev Phytopath, 12: 167–179.
Ishimine Y, Shinjyo C. 1978. Distribution of fertility restoring genes in commercial lowland-rice varieties cultivated in Japan for two male sterile cytoplasm of rice. Bull Coll Agric Univ Ryukyus, 25: 665–672.
Li S Q, Yang D C, Zhu Y G. 2007. Characterization and use of male sterility in hybrid rice breeding. J Integr Plant Biol, 49(6): 791–804.
Li X Q, Xu Y B, Xiao J H, Yuan L P, McCouch S. 2010. Studies on mapping and discarding of minor fertility genes in rice CMS lines. Hybrid Rice, 25(2): 53–58. (in Chinese)
Liang G H, Zhou P N, Yang H, Tang S Z, Gu M H. 2002. Screening and classification of the restorer varieties for HL-CMS line. J Yangzhou Univ: Agric Life Sci, 23(3): 41–45. (in Chinese with English abstract)
Liu X Q, Xu X, Tan Y P, Li S Q, Hu J, Huang J Y, Yang D C, Li Y S, Zhu Y G. 2004. Inheritance and molecular mapping of two fertility-restoring loci for Honglian gametophytic cytoplasmic male sterility in rice (Oryza sativa L.). Mol Genet Genom, 271(5): 586–594.
Sattari M, Kathiresan A, Gregorio G B, Virmani S S. 2008. Comparative genetic analysis and molecular mapping of fertility restoration genes for WA, Dissi, and Gambiaca cytoplasmic male sterility systems in rice. Euphytica, 160(3): 305–315.
Shinjyo C. 1969. Cytoplasmic genetic male sterility in cultivated rice, Oryza sativa L: II. The inheritance of male sterility. Jpn J Genet, 44: 149–156.
Wang N Y. 2006. Studies on improvement of sterile line rice quality by new cytoplasm from Oryza rufipogon. Acta Agron Sin, 32(2): 253–259. (in Chinese with English abstract)
Wang N Y, Liang K J, Li Y, Wang Y, Wang H F, Qiu X L, Wei X Y, Zhang Y Y, Ke B, Zhao J Y. 2008a. Screening parent resources of hybrid rice in new cytoplasm male sterile system (CMS-FA). Acta Agron Sin, 34(9): 1549–1556. (in Chinese with English abstract)
Wang N Y, Liang K J, Li Y, Wang Y, Wang H F, Qiu X L. 2008b. Inheritance of restorer gene for CMS-FA hybrid rice. Acta Agron Sin, 34(11): 1929–1937. (in Chinese with English abstract)
Wang Z H, Zou Y J, Li X Y, Zhang Q Y, Chen L T, Wu H, Su D H, Chen Y L, Guo J X, Luo D, Long Y M, Zhong Y, Liu Y G. 2006. Cytoplasmic male sterility of rice with Boro II cytoplasm is caused by a cytoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. Plant Cell, 18(3): 676–687.
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