Research Paper

Fine Mapping and Cloning of Leafy Head Mutant Gene pla1-5 in Rice

Expand
  • 1 Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou 225009, China; 2 Yancheng Institute of Technology, Yancheng 224051, China

Online published: 2013-07-30

Supported by

This study was financially supported by grants from the Distinguished Young Scientists from Jiangsu Government, China (Grant No. BK2012010), the Key Project of Chinese Ministry of Education, and the Ministry of Science and Technology of China (Grant Nos. 2012AA10A302-7 and 2013ZX08009-003).

Abstract

We identified a leafy head mutant pla1-5 (plastochron 1-5) from the progeny of japonica rice cultivar Taipei 309 treated with 60Co-γ ray irradiation. The pla1-5 mutant has a dwarf phenotype and small leaves. Compared with its wild type, pla1-5 has more leaves and fewer tillers, and it fails to produce normal panicles at the maturity stage. Genetic analysis showed that the pla1-5 phenotype is controlled by a single recessive nuclear gene. Using the map-based cloning strategy, we narrowed down the location of the target gene to a 58-kb region between simple sequence repeat markers CHR1027 and CHR1030 on the long arm of chromosome 10. The target gene cosegregated with molecular markers CHR1028 and CHR1029. There were five predicted genes in the mapped region. The results from sequencing analysis revealed that there was one base deletion in the first exon of LOC_Os10g26340 encoding cytochrome P450 CYP78A11 in the pla1-5 mutant, which might result in a downstream frame shift and premature termination. These results suggest that the P450 CYP78A11 gene is the candidate gene of PLA1-5.

Cite this article

FENG Gong-Neng, ZHANG Chang-Quan, ZHAO Dong-Sheng, ZHU Kong-Zhi, TU Huai-Zhou, XU Chen-Wu, LIU Qiao-Quan . Fine Mapping and Cloning of Leafy Head Mutant Gene pla1-5 in Rice[J]. Rice Science, 2013 , 20(5) : 329 -335 . DOI: 10.1016/S1672-6308(13)60140-7

References

Bowman J L, Alvarez J, Weigel D, Meyerowitz E M, Smyth D R. 1993. Control of flower development in Arabidopsis thaliana by APETA1 and interacting genes. Development, 119(3): 721–743.
Chaudhury A M, Letham S, Craig S, Dennis E S. 1993. amp1: A mutant with high cytokinin levels and altered embryonic pattern, faster vegetative growth, constitutive photomorphogenesis and precocious flowering. Plant J, 4(6): 907–916.
Chu H W, Qian Q, Liang W Q, Yin C S, Tan H X, Yao X, Yuan Z, Yang J, Huang H, Luo D, Ma H, Zhang D B. 2006. The FLORAL ORGAN NUMBER4 gene encoding a putative ortholog of Arabidopsis CLAVATA3 regulates apical meristem size in rice. Plant Physiol, 142(3): 1039–1052.
Dellaporta S L, Wood J, Hicks J B. 1983. A plant DNA minipreparation: Version II. Plant Mol Biol Rep, 1(4): 19–21.
Duan Y L, Li W M, Wu W R, Pan R S, Zhou Y C, Qi J M, Ling L H, Chen Z W, Mao D M, Liu H Q, Zhang D F, Xue Y B. 2003a. Genetic analysis and molecular marker mapping of rice panicle differentiation regulating gene fzp(t). Sci China: Ser C, 33(1): 27–32. (in Chinese)
Duan Y L, Wu W R, Liu H Q, Zhang D F, Zhou Y C, Pan R S, Lin L H, Chen Z W, Guan H Z, Mao D M, Li W M, Xue Y B. 2003b. Genetic analysis and gene mapping of leafy head (lhd), a mutant blocking the differentiation of rachis branches in rice (Oryza sativa L.). Chin Sci Bull, 48(20): 2201–2205.
Evans M M S, Poethig R S. 1997. The viviparous8 mutation delays vegetative phase change and accelerates the rate of seedling growth in maize. Plant J, 12(4): 769–779.
Gao Z S, Sugita S, Ikeda S, Cai H W, Sasaki T, Liang G H. 2002. Linkage of AFLP markers to lhd 1, a recessive heterochronic gene in Italian ryegrass. Genome, 45(4): 752–758.
Guan H Z, Duan Y L, Liu H Q, Chen Z W, Zhuo M, Zhuang L J, Qi W M, Pan R S, Mao D M, Zhou Y C, Wang F, Wu W R. 2011. Genetic analysis and fine mapping of an enclosed panicle mutant esp2 in rice (Oryza sativa L.). Chin Sci Bull, 56(14): 1476–1480.
Hu C H. 1961. An X-ray induced panicle-degenerating mutant in rice. Jpn J Breeding, 11(1): 19–23. (in Japanese with English summary)
Irish V F. 2010. The flowering of Arabidopsis flower development. Plant J, 61(6): 1014–1028.
Itoh J I, Hasegawa A, Kitano H, Nagato Y. 1998. A recessive heterochronic mutation, plastochron 1, shortens the plastochron and elongates the vegetative phase in rice. Plant Cell, 10(9): 1511–1521.
Jiang L, Qian Q, Mao L, Zhou Q Y, Zhai W X. 2005. Characterization of the rice floral organ number mutant fon3. J Integr Plant Biol, 47(1): 100–106.
Kawakatsu T, Itoh J I, Miyoshi K, Kurata N, Alvarez N, Veit B, Nagato Y. 2006. PLASTOCHRON 2 regulates leaf initiation and maturation in rice. Plant Cell, 18(3): 612–625.
Kawakatsu T, Taramino G, Itoh J I, Allen J, Sato Y, Hong S K, Yule R, Nagasawa N, Kojima M, Kusaba M, Sakakibara H, Sakai H, Nagato Y. 2009. PLASTOCHRON3/GOLIATH encodes a glutamate carboxypeptidase required for proper development in rice. Plant J, 58(6): 1028–1040.
Kaufmann K, Mui?o J M, Jauregui R, Airoldi C A, Smaczniak C, Krajewski P, Angenent G C. 2009. Target genes of the MADS transcription factor SEPALLATA3: Integration of developmental and hormonal pathways in the Arabidopsis flower. PLoS Biol, 7: e1000090.
Komatsu M, Chujo A, Nagato Y, Shimamoto K, Kyozuka J. 2003. FRIZZY PANICLE is required to prevent the formation of axillary meristems and to establish floral meristem identity in rice spikelets. Development, 130(16): 3841–3850.
Li S B, Qian Q, Fu Z M, Zeng D L, Meng X B, Kyozuka J, Maekawa M, Zhu X D, Zhang J, Li J Y, Wang Y H. 2009. Short panicle1 encodes a putative PTR family transporter and determines rice panicle size. Plant J, 58(4): 592–605.
Michelmore R W, Paran I, Kesseli R V. 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 88(21): 9828–9832.
Mimura M, Nagato Y, Itoh J I. 2012. Rice PLASTOCHRON genes regulate leaf maturation downstream of the gibberellin signal transduction pathway. Planta, 235(5): 1081–1089.
Miyoshi K, Ahn B O, Kawakatsu T, Ito Y, Itoh J I, Nagato Y, Kurata N. 2004. PLASTOCHRON 1, a timekeeper of leaf initiation in rice, encodes cytochrome P450. Proc Natl Acad Sci USA, 101(3): 875–880.
Moon S, Jung K H, Lee D E, Lee D Y, Lee J, An K, Kang H G, An G. 2006. The rice FON1 gene controls vegetative and reproductive development by regulating shoot apical meristem size. Mol Cells, 21(1): 147–152.
Schuler M A. 1996. Plant cytochrome P450 monooxygenases. Crit Rev Plant Sci, 15(3): 235–284.
Suzaki T, Toriba T, Fujimoto M, Tsutsumi N, Kitano H, Hirano H Y. 2006. Conservation and diversification of meristem maintenance mechanism in Oryza sativa: Function of the FLORAL ORGAN NUMBER2 gene. Plant Cell Physiol, 47(12): 1591–1602.
Wu L W, Jiang H, Hu J, Hu X M, Gao Z Y, Zeng D L, Zhu G L, Dong G J, Qian Q, Guo L B. 2009. Genetic analysis and fine- mapping of a Leafy Head 3 mutant in rice. Chin J Rice Sci, 23(6): 583–588. (in Chinese with English abstract)
Xiong G S, Hu X M, Jiao Y Q, Yu Y C, Chu C C, Li J Y, Qian Q, Wang Y H. 2006. LEAFY HEAD 2, which encodes a putative RNA-binding protein, regulates shoot development of rice. Cell Res, 16(3): 267–276.
Yant L, Mathieu J, Dinh T T, Ott F, Lanz C, Wollmann H, Chen X M, Schmid M. 2010. Orchestration of the floral transition and floral development in Arabidopsis by the bifunctional transcription factor APETALA2. Plant Cell, 22(7): 2156–2170.
Zhu Q H, Hoque M S, Dennis E S, Upadhyaya N M. 2003. Ds tagging of BRANCHED FLORETLESS 1 (BFL1) that mediates the transition from spikelet to floret meristem in rice (Oryza sativa L). BMC Plant Biol, 3(1): 6.
Zhu Q H, Dennis E S, Upadhyaya N M. 2007. compact shoot and leafy head 1, a mutation affects leaf initiation and developmental transition in rice (Oryza sativa L.). Plant Cell Rep, 26(4): 421– 427.
Outlines

/

浙ICP备05004719号-15   公安备案号:33010302003355
Copyright © Editorial office of Rice Science
Tel: 0571-63371017 E-mail: crrn@fy.hz.zn.cn; cjrs278@gmail.com
Supported by Beijing Magtech Co., Ltd.
Total visitors:  Visitors of today:  Now online: