Photosynthetic Characteristics of Flag Leaves in Rice White Stripe Mutant 6001 During Senescence Process

ZHEN Xiao-hui, XU Jin-gang, SHEN Wei-jun, ZHANG Xiao-juan, ZHANG Qi-jun, LU Chuan-gen, CHEN Guo-xiang, GAO Zhi-ping

doi:10.1016/S1672-6308(14)60268-7

Rice Science >

2014 , Vol. 21 >Issue 6: 335 - 342

DOI: https://doi.org/10.1016/S1672-6308(14)60268-7

Research Paper

Photosynthetic Characteristics of Flag Leaves in Rice White Stripe Mutant 6001 During Senescence Process

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College of Life Sciences, Nanjing Normal University, Nanjing 210097, China; Institute of Food and Crops, Jiangsu Academy of Agricultural Science, Nanjing 210014, China; 3Nanjing Sport Institute, Nanjing 210014, China

Online published: 2014-09-26

Supported by

This research is supported by the National Natural Science Foundation of China (Grant No. 31271621), the Natural Science Foundation of the Jiangsu Ordinary Higher Education Institutions of China (Grant No. 11KJA180001), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), China.

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Abstract

Physiological, biochemical and electron microscopy analyses were used to investigate the photosynthetic performance of flag leaves in rice white stripe mutant 6001 during the senescence process. Results showed that the chlorophyll content at the heading and milk-ripe stages in rice mutant 6001 were about 34.78% and 3.00% less than those in wild type 6028, respectively. However, the chlorophyll content at the fully-ripe stage in rice mutant 6001 was higher than that in wild type 6028. At the heading stage, the net photosynthetic rate (Pn) in rice mutant 6001 was lower than that in wild type 6028. Rice mutant 6001 also exhibited a significantly slower decrease rate of Pn than wild type 6028 during the senescence progress, especially at the later stage. Furthermore, Ca2+-ATPase, Mg2+-ATPase and photophosphorylation activities exhibited the similar trends as the Pn. During the senescence process, the 68 kDa polypeptide concentrations in the thylakoid membrane proteins exhibited a significant change, which was one of the critical factors that contributed to the observed change in photosynthesis. We also observed that the chloroplasts of rice mutant 6001 exhibited higher integrity than those of wild type 6028, and the chloroplast membrane of rice mutant 6001 disintegrated more slow during the senescence process. In general, rice mutant 6001 had a relatively slower senescence rate than wild type 6028, and exhibited anti-senescence properties.

Key words： chlorophyll content; fluorescence emission spectrum; net photosynthetic rate; polypeptide component; photophosphorylation; rice mutant; ultrastructure

Cite this article

ZHEN Xiao-hui, XU Jin-gang, SHEN Wei-jun, ZHANG Xiao-juan, ZHANG Qi-jun, LU Chuan-gen, CHEN Guo-xiang, GAO Zhi-ping . Photosynthetic Characteristics of Flag Leaves in Rice White Stripe Mutant 6001 During Senescence Process[J]. Rice Science, 2014 , 21(6) : 335 -342 . DOI: 10.1016/S1672-6308(14)60268-7

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