Research Paper

Reverse Genetic Analysis of Transcription Factor OsHox9, a Member of Homeobox Family, in Rice

Expand
  • Tianjin Normal University, College of Life Science, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin 300387, China

Online published: 2014-09-26

Supported by

This work was supported by the National Natural Science Foundation of China (Grant NO. 31171515), the Tianjin Natural Science Foundation of China (Grant NO. 11JCZDJC17900), and the Knowledge Innovation and Training Program of Tianjin, Tianjin Municipal Education Commission, China (Grant NO. 2013-1-2015-12).

Abstract

Homeobox transcription factors participate in the growth and development of plants by regulating cell differentiation, morphogenesis and environmental signal response. To reveal the functions of these transcription factors in rice, we constructed the RNAi vectors of OsHox9, a member of homeobox family, and analyzed the function of OsHox9 using reverse genetics. The plant height and tillering number of RNAi transgenic plants decreased compared with those of wild-type plants. Reverse transcription-polymerase chain reaction analysis showed that OsHox9 expression reduced in the transgenic plants with phenotypic variance, whereas that in the transgenic plants without phenotypic variance was similar to that in the wild-type plants. This result suggests that the phenotypes of the transgenic plants were caused by RNAi effects. The tissue-specificity of OsHox9 expression indicated that it was expressed in different organs, with high expression in stem apical meristem and young panicles. Subcellular location of OsHox9 demonstrated that it was localized on the cell membrane.

Cite this article

AI Li-ping, SHEN Ao, GAO Zhi-chao, LI Zheng-long, SUN Qiong-lin, LI Ying-ying, LUAN Wei-jiang . Reverse Genetic Analysis of Transcription Factor OsHox9, a Member of Homeobox Family, in Rice[J]. Rice Science, 2014 , 21(6) : 312 -317 . DOI: 10.1016/S1672-6308(14)60271-7

References

Ariel F D, Manavella P A, Dezar C A, Chan R L. 2007. The true story of the HD-Zip family. Trends Plant Sci, 12(9): 419–426.
Carabelli M, Sessa G, Baima S, Morelli G, Ruberti I. 1993. The Arabidopsis Athb-2 and -4 genes are strongly induced by far-red-rich light. Plant J, 4(3): 469–479.
Chan R L, Gago G M, Palena C M, Gonzalez D H, 1998. Homeoboxes in plant development. Biochim Biophys Acta, 1442(1): 1–19.
Chao X, Gong Z H, Lu M H, Ma C, Li D W, Zhao J, Meng C J. 2008. Cloning and analysis of cellar location of the Arabidopsis heat transcriptional factor AtHsfA6a. J Northwest A & F Univ, 36(4): 94–98. (in Chinese with English abstract)
Gao Z C, and Luan W J. 2012. Characterization and expression analysis of a Ds-tagging line OsPI-PLC2 in rice. J Tianjin Norm Univ, 32(3): 85–90. (in Chinese with English abstract)
Hiei Y, Ohta S, Komari T, Kumashiro T. 1994. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J, 6(2): 271–282.
Ingram G C, Boisnard-Lorig C, Dumas C, Rogowsky P M. 2000. Expression patterns of genes encoding HD-Zip IV homeo domain proteins define specific domains in maize embryos and meristems. Plant J, 22(5): 401–414.
Itoh J I, Hibara K I, Sato Y, Nagato Y. 2008. Developmental role and auxin responsiveness of class III homeodomain leucine zipper gene family members in rice. Plant Physiol, 147(4): 1960–1975.
Johannesson N, Wang Y, Engstr?m P. 2001. DNA-binding and dimerization preferences of Arabidopsis homeodomain-leucine zipper transcription factors in vitro. Plant Mol Biol, 45(1): 63–73.
Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta (T)) method. Methods, 25: 402–408.
Li G X, Wu M S, Wu J, He C Y. 2009. Molecular identification and characterization of a rice gene of OsBTF3 encoding a transcriptional factor up-regulated by Xanthomonas oryzae pv. Oryzae. Sci Agr Sin, 42(7): 2608–2614. (in Chinese with English abstract)
Luan W J, Shen A, Jin Z P, Song S S, Li Z L, Sha A H. 2013. Knockdown of OsHox33, a member of the class III homeodomain-leucine zipper gene family, accelerates leaf senescence in rice. Sci China Life Sci, 56: 1–11.
Meijer A H, Scarpella E, van Dijk E L, Qin L, Taal A J, Rueb S, Harrington S E, McCouch S R, Schilperoort R A, Hoge J H. 1997. Transcriptional repression by Oshox1, a novel homeodomain leucine zipper protein from rice. Plant J, 11(2): 263–276.
Ohashi-Ito K, Demura T, Fukuda H. 2002. Promotion of transcript accumulation of novel Zinnia immature xylem-specific HD-Zip III homeobox genes by brassinosteroids. Plant Cell Physiol, 43(10): 1146–1153.
Prigge M J, Otstuga D, Alonso J M, Ecker J, Drews G N, Clark S E. 2005. Class III homeodomain-leucine zipper gene family members have overlapping, antagonistic, and distinct roles in Arabidopsis development. Plant Cell, 17(1): 61–76.
Ramachandran S, Hiratsuka K, Chua N H. 1994. Transcription factors in plant growth and development. Curr Opin Genet Dev, 4(5): 642–646.
Sessa G, Steindler C, Morelli G, Ruberti I. 1998. The Arabidopsis Athb-8, -9 and -14 genes are members of a small gene family coding for highly related HD-ZIP proteins. Plant Mol Biol, 38(4): 609–622.
Soderman E, Hjellstrom M, Fahleson J, Engstrom P. 1998. The HD-Zip gene ATHB6 in Arabidopsis is expressed in developing leaves, roots and carpels and up-regulated by water deficit conditions. Plant Mol Biol, 40(6): 1073–1083.
Soderman E, Mattsson J, Engstrom P. 1996. The Arabidopsis homeobox gene ATHB-7 is induced by water deficit and by abscisic acid. Plant J, 10(2): 375–381.
Tornero P, Conejero V, Vera P. 1996. Phloem-specific expression of a plant homeobox gene during secondary phases of vascular development. Plant J, 9(5): 639–648.
Outlines

/

浙ICP备05004719号-15   公安备案号:33010302003355
Copyright © Editorial office of Rice Science
Tel: 0571-63371017 E-mail: crrn@fy.hz.zn.cn; cjrs278@gmail.com
Supported by Beijing Magtech Co., Ltd.
Total visitors:  Visitors of today:  Now online: