Improvement of Rice Resistance to Sheath Blight by Pyramiding QTLs Conditioning Disease Resistance and Tiller Angle

ZUO Shi-min, ZHANG Ya-fang, CHEN Zong-xiang, JIANG Wei, FENG Ming-hui, PAN Xue-biao

doi:10.1016/S1672-6308(14)60274-2

Rice Science >

2014 , Vol. 21 >Issue 6: 318 - 326

DOI: https://doi.org/10.1016/S1672-6308(14)60274-2

Research Paper

Improvement of Rice Resistance to Sheath Blight by Pyramiding QTLs Conditioning Disease Resistance and Tiller Angle

Expand

Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou 225009, China

Online published: 2014-09-26

Supported by

This work was partially supported by the Key Project of National Science foundation of Jiangsu Higher Education Institutions, China (Grant No. 14KJA210003), the National Transgenic Projects (Grant No. 2014ZX08001-003B), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), China.

Fold

Abstract

Rice resistance to sheath blight (SB) is controlled by polygenes or quantitative trait loci (QTLs) and affected by plant morphological traits. qSB-9TQ and TAC1TQ, which control SB resistance and tiller angle (TA), respectively, were employed to test whether the combination of the two genes will further improve rice SB resistance and reduce yield loss rather than only one of them or neither. Using two pairs of near isogenic lines (NILs), TAC1TQ was confirmed to contribute to SB resistance. However, its effect was less considerable than that of qSB-9TQ. Subsequently, the two genes were introduced into two commercial rice varieties to develop a series of NILs. The NILs carrying both TAC1TQ and qSB-9TQ showed more resistance than the NILs containing only one of them. Comparing the grain yield per plant (GYP) under the three different SB disease conditions, namely slight, moderate and severe conditions, NILs carrying both genes apparently lost lower GYP than the NILs without the two genes and the NILs carrying only TAC1TQ. Under slight disease condition, no significant differences were found on morphology, development and GYP associated traits except for TA between the NILs carrying both genes and either of them, indicating that the two genes have no inferior effect on rice agronomic traits. Results indicated that TAC1TQ and qSB-9TQ have high breeding potential, and pyramiding SB resistance QTL and morphological trait QTL is a potential approach in improving rice SB resistance.

Key words： rice; sheath blight; QTL pyramiding; breeding potential; yield

Cite this article

ZUO Shi-min, ZHANG Ya-fang, CHEN Zong-xiang, JIANG Wei, FENG Ming-hui, PAN Xue-biao . Improvement of Rice Resistance to Sheath Blight by Pyramiding QTLs Conditioning Disease Resistance and Tiller Angle[J]. Rice Science, 2014 , 21(6) : 318 -326 . DOI: 10.1016/S1672-6308(14)60274-2

References

Chen Z X, Zhang Y F, Feng F, Feng M H, Jiang W, Ma Y Y, Pan C, Hua H L, Li G S, Pan X B, Zuo S M. 2014. Improvement of japonica rice resistance to sheath blight by pyramiding qSB-9TQ and qSB-7TQ. Field Crop Res, 161: 118–127.
Chen Z X, Zuo S M, Zhang Y F, Zhu J K, Wang L P, Feng F, Ma Y Y, Pan X B. 2012. Rice pyramiding breeding using sheath blight resistance QTL qSB-9TQ and stripe disease resistance gene Stv-bi. Acta Agron Sin, 38: 1178–1186.
Han Y P, Xing Y Z, Gu S L, Chen Z X, Pan X B, Chen X L. 2003. Effect of morphological traits on sheath blight resistance in rice. Acta Bot Sini, 45(7): 825–831.
Lee F N, Rush M C. 1983. Rice sheath blight: A major rice disease. Plant Dis, 67: 829–832.
Li Z K, Pinson S R M, Marshetti M A, Stansel J W, Park W D. 1995. Characterization of quantitative trait loci (QTLs) in cultivated rice contributing to field resistance to sheath blight (Rhizoctonia solani). Theor Appl Genet, 91: 382–388.
Liu G J, Jia Y L, McClung A, Oard J H, Lee F N, Correll J C. 2013. Confirming QTLs and finding additional loci responsible for resistance to rice sheath blight disease. Plant Dis, 97(1): 113–117.
Marchetti M A, Bollich C N. 1991. Quantification of the relationship between sheath blight severity and yield loss in rice. Plant Dis, 75: 773–775.
Pinson S R M, Capdevielle F M, Oard J H. 2005. Confirming QTLs and finding additional loci conditioning sheath blight resistance in rice using recombinant inbred lines. Crop Sci, 45: 503–510.
Sharma A, McClung A M, Pinson S R M, Kepiro J L, Shank A R, Tabien R E, Fjellstrom R. 2009. Genetic mapping of sheath blight resistance QTLs within tropical japonica rice cultivars. Crop Sci, 49: 256–264.
Srinivasachary, Willocquet L, Savary S. 2011. Resistance to rice sheath blight (Rhizoctonia solani Kühn) [(teleomorph: Thanatephorus cucumeris (A. B. Frank) Donk.] disease: Current status and perspectives. Euphytica, 178: 1–22.
Taguchi-Shiobara F, Ozaki H, Sato H, Maeda H, Kojima Y, Ebitani T, Yano M. 2013. Mapping and validation of QTLs for rice sheath blight resistance. Breeding Sci, 63(3): 301–308.
Tan C X, Ji X M, Yang Y, Pan X Y, Zuo S M, Zhang Y F, Zou J H, Chen Z X, Zhu L H, Pan X B. 2005. Identification and marker assisted selection of two major quantitative genes controlling rice sheath blight resistance in backcross generations. Acta Genet Sin, 32: 399–405.
Wang Y, Pinson S R M, Fjellstrom R G, Tabien R E. 2012. Phenotypic gain from introgression of two QTL, qSB9-2 and qSB12-1, for rice sheath blight resistance. Mol Breeding, 30: 293–303.
Yin Y J, Zuo S M, Wang H, Chen Z X, Gu S L, Zhang Y F, Pan X B. 2009. Evaluation of the effect of qSB-9(TQ) involved in quantitative resistance to rice sheath blight using near-isogenic lines. Can J Plant Sci, 4: 731–737.
Yu B S, Lin Z W, Li H X, Li X J, Li J Y, Wang Y H, Zhang X, Zhu Z F, Zhai W X, Wang X K, Xie D X, Sun C Q. 2007. TAC1, a major quantitative trait locus controlling tiller angle in rice. Plant J, 52: 891–898.
Zhen A P, Lin R M, Zhang D H, Qin P G, Xu L Z, Ai P, Ding L, Wang Y N, Chen Y, Liu Y, Sun Z G, Feng H T, Liang X X, Fu R T, Tang C G, Li Q, Zhang J, Xie Z L, Deng Q M, Li S C, Wang S Q, Zhu J, Wang L X, Liu H N, Li P. 2013. The evolution and pathogenic mechanisms of the rice sheath blight pathogen. Nat Comm, 4: 1424–1433.
Zhu Y J, Zuo S M, Chen Z X, Chen X G, Li G, Zhang Y F, Zhang G Q, Pan X B. 2014. Identification of two major rice sheath blight resistance QTLs, qSB1-1HJX74 and qSB11HJX74, in field trials using chromosome segment substitution lines. Plant Dis, 98(8): 1112–1130.
Zou J H, Pan X B, Chen Z X, Xu J Y, Lu J F, Zhai W X, Zhu L H. 2000. Mapping quantitative trait loci controlling sheath blight resistance in two rice cultivars (Oryza sativa L.). Theor Appl Genet, 101: 569–575.
Zuo S M, Yin Y J, Pan C H, Chen Z X, Zhang Y F, Gu S L, Zhu L H, Pan X B. 2013. Fine mapping of qSB-11LE, the QTL that confers partial resistance to rice sheath blight. Theor Appl Genet, 126: 1257–1272.
Zuo S M, Yin Y J, Zhang L, Zhang Y F, Chen Z X, Pan X B. 2007. Breeding value and further mapping of a QTL qSB-11 conferring rice sheath blight resistance. Chin J Rice Sci, 21: 136–142. (in Chinese with English abstract)
Zuo S M, Zhang L, Wang H, Yin Y J, Zhang Y F, Chen Z X, Ma Y Y, Pan X B. 2008. Prospect of the QTL-qSB9Tq utilized in molecular breeding program of japonica rice against sheath blight. J Genet Genom, 35: 499–505.
Zuo S M, Zhang Y F, Chen Z X, Chen X J, Pan X B. 2010. Current progress on genetics and breeding in resistance to rice sheath blight. Sci Sin Vitae, 40(11): 1014–1023.
Zuo S M, Zhang Y F, Yin Y J, Li G Z, Zhang G W, Wang H, Chen Z X, Pan X B. 2014a. Fine-mapping of qSB-9TQ, a gene conferring major quantitative resistance to rice sheath blight. Mol Breeding, DOI: 10.1007/s11032-014-0173-5.
Zuo S M, Zhu Y J, Yin Y J, Wang H, Zhang Y F, Chen Z X, Gu S L, Pan X B. 2014b. Comparison and confirmation of quantitative trait loci conferring partial resistance to rice sheath blight on chromosome 9. Plant Dis, 98(7): 957–964.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References