RL3(t), Responsible for Leaf Shape Formation, Delimited to a 46-kb DNA Fragment in Rice
Received date: 2014-06-17
Accepted date: 2014-10-03
Online published: 2014-11-26
Two mutants with rolled leaves, temporally designated as rl3(t)-1 and rl3(t)-2, were served for exploring the mechanism underlying the rolled leaf characteristic. Except for having typical rolled leaves, the plant heights and panicle lengths of rl3(t)-1 and rl3(t)-2 significantly decreased, and the seed-setting rate also decreased when compared with wild type 93-11. Cytological analysis suggested that the rolled leaf phenotype might be caused by the changes of number and size of bulliform cells. Genetic analysis indicated rl3(t)-1 is allelic to rl3(t)-2, and controlled by a recessive gene. Gene mapping result indicated that RL3(t) gene resided in a 46-kb long region governed by the sequence tag site markers S3-39 and S3-36 on rice chromosome 3. The result provides an important clue for further cloning the RL3(t) and understanding the mechanism of rice leaf development.
Key words： gene mapping; leaf shape formation; mutant; rice; rolled leaf gene
Min Guo, Rong-de Li, Jian Yao, Juan Zhu, Xiang-yun Fan, Wei Wang, Shu-zhu Tang, Ming-hong Gu, Chang-jie Yan . RL3(t), Responsible for Leaf Shape Formation, Delimited to a 46-kb DNA Fragment in Rice[J]. Rice Science, 2015 , 22(1) : 44 -48 . DOI: 10.1016/S1672-6308(14)60276-6
| 1 | Chen D B, Cheng S H, Cao L Y.2010. Research progress on narrow leaf trait in rice.China Rice, 16(3): 1-4. (in Chinese) |
| 2 | Chen W F, Xu Z J, Zhang L B.1989. Research on rice ideotype.J Shenyang Agric Univ, 20(4): 417-420. (in Chinese) |
| 3 | Fang L K, Zhao F M, Cong Y F, Song X C, Du Q, Wang D Z, Li Y F, Ling Y H, Yang Z L, He G H.2012. Rolling-leaf14 is a 2OG-Fe (II) oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves.Plant Biotechnol J, 10(5): 524-532. |
| 4 | Gao Y H, Lv C G.2006. Status of studies on rice rolled leaf.J Jinling Inst Technol, 1: 62-66. (in Chinese with English abstract) |
| 5 | Hibara K, Obara M, Hayashida E, Abe M, Ishimaru T, Satoh H, Itoh J, Nagato Y.2009. The ADAXIALIZED LEAF1 gene functions in leaf and embryonic pattern formation in rice.Dev Biol, 334(2): 345-354. |
| 6 | Kang W Q, Ouyang Y N, Dong C Q, Zhu L F, Yu S M, Xu D H, Jiu Q Y.2007. Discussion on the mode and index for rice dynamic plant type.China Rice, 13(1): 1-6. (in Chinese) |
| 7 | Kinoshita T.1990. Report of the committee on gene symbolization, nomenclature and linkage groups.Rice Genet Newsl, 7: 16-50. |
| 8 | Li L, Shi Z Y, Li L, Shen G Z, Wang X Q, An L S, Zhang J L.2010. Overexpression of ACL1 (abaxially curled leaf 1) increased bulliform cells and induced abaxial curling of leaf blades in rice.Mol Plant, 3(5): 807-817. |
| 9 | Li M, Xiong G Y, Li R, Cui J J, Tang D, Zhang B C, Pauly M, Cheng Z K, Zhou Y H.2009. Rice cellulose synthase-like D4 is essential for normal cell-wall biosynthesis and plant growth.Plant J, 60(6): 1055-1069. |
| 10 | Lv C G, Gu F L, Zou J S, Lu M L.1991. Studies on yielding potential and related characterstics of rice ideotype.Sci Agric Sin, 24(5): 15-22. (in Chinese with English abstract) |
| 11 | Matsushita S, Iseki T, Fukuta Y, Araki E, Kobayashi S, Osaki M, Yamgishi M.2003. Characterization of segregation distortion on chromosome 3 induced in wide hybridization between indica and japonica type rice varieties.Euphytica, 134: 27-32. |
| 12 | Peng Y, Liang Y S, Wang S Q, Wu F Q, Li S C, Deng Q M, Li P.2006. Analysis of segregation distortion of SSR markers in the RI population of rice.Mol Plant Breeding, 4: 786-790. (in Chinese with English abstract) |
| 13 | Shi Z Y, Wang J, Wan X S, Shen G Z, Wang X Q, Zhang J L.2007. Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit.Planta, 226: 99-108. |
| 14 | Su Z F, Xu N X, Sun C M, Zhang Y J.2003. Study on the relationship between rice plant type indices after heading stage and yield formation.Sci Agric Sin, 36(1): 115-120. (in Chinese with English abstract) |
| 15 | Wang D Z, Sang X C, You X Q, Wang Z, Wang Q Z, Zhao F M, Ling Y H, Li Y F, He G H.2011. Genetic analysis and gene mapping of a novel and rolled leaf mutant nrl2(t) in rice (Oryza sativa L.). Acta Agron Sin, 37(7): 1159-1166. (in Chinese with English abstract) |
| 16 | Xiang J J, Zhang G H, Qian Q, Xue H W.2012. Semi-rolled leaf1 encodes a putative glycosylphosphatidylinositol-anchored protein and modulates rice leaf rolling by regulating the formation of bulliform cells.Plant Physiol, 159(4): 1488-1500. |
| 17 | Xu Y, Zhu L, Xiao J, Huang N, McCouch S R.1997. Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, doubled haploid, and recombinant inbred populations in rice (Oryza sativa L.).Mol Gen Genet, 253(3): 535-545. |
| 18 | Yan S, Yan C J, Zeng X H, Yang Y C, Fang Y W, Tian C Y, Sun Y W, Cheng Z K, Gu M H.2008.ROLLED LEAF 9, encoding a GARP protein, regulates the leaf abaxial cell fate in rice.Plant Mol Biol, 68(3): 239-250. |
| 19 | Yan S, Yan C J, Gu M H.2008. Molecular mechanism of leaf development.Hereditas, 30(9): 1127-1135. |
| 20 | Yuan L P.1997. Hybrid rice breeding for super high yield.Hybrid Rice, 12(6): 1-6. (in Chinese) |
| 21 | Zhang G H, Xu Qian, Zhu X D, Qian Q, Xue H W.2009. SHALLOT-LIKE1 is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development.Plant Cell, 21(3): 719-735. |
| 22 | Zou L P, Sun X H, Zhang Z G, Liu P, Wu J X, Tian C J, Qiu J L, Lu T G.2011. Leaf rolling controlled by the homeodomain leucine zipper class IV gene ROC5 in rice.Plant Physiol, 156(3): 1589-1602. |
| 23 | Zuo J R, Li J Y.2014. Molecular dissection of complex agronomic traits of rice: A team effort by Chinese scientists in recent years.Nat Sci Rev, 1: 253-276. |
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