Identification and Characterization of OsWRKY72 Variant in Indica Genotypes
Received date: 2016-01-07
Accepted date: 2016-07-04
Online published: 2016-08-10
Plant WRKY transcription factors (TFs) constitute one of the largest families of proteins involved in biotic and abiotic stress responses. These TFs have a conserved 60 amino acid WRKY domain at the N-terminal and a zinc finger motif at the C-terminal. To examine the relevance of OsWRKY72 in imparting salinity stress tolerance, two indica rice genotypes, Rasi (tolerant genotype) and Tellahamsa (susceptible genotype), were used. In Rasi seedlings at 12 h under 100 mmol/L NaCl stress, OsWRKY72 expression was up-regulated, whereas in Tellahamsa, it was highly up-regulated at lethal stress. Full-length OsWRKY72 cDNA was cloned from these two rice genotypes for further analysis. We identified a variant, termed as OsWRKY72b that carries an additional sequence of 111 bp within the WRKY domain. Expression of OsWRKY72b was higher under salinity stress in Rasi than in Tellahamsa. Disorder prediction of OsWRKY72b showed that the additional sequence in the WRKY domain is ordered thereby maintaining the tertiary structure that might interact with the major groove of DNA. Prediction of phosphorylation sites in OsWRKY72b indicated that a few serine residues could be the potential phosphorylation sites. In this study, we firstly reported a OsWRKY72 variant that could have a role in abiotic stress responses.
Key words： Oryza sativa; indica genotype; OsWRKY72 gene; salinity; transcription factor
Ashwini Narasimha, Sivarajan Sajeevan Radha, Udayakumar Makarla, Nalkur Nataraja Karaba . Identification and Characterization of OsWRKY72 Variant in Indica Genotypes[J]. Rice Science, 2016 , 23(6) : 297 -305 . DOI: 10.1016/j.rsci.2016.07.002
| 1 | Agarwal P, Reddy M P, Chikara J.2011. WRKY: Its structure, evolutionary relationship, DNA-binding selectivity, role in stress tolerance and development of plants.Mol Biol Rep, 38: 3883-3896. |
| 2 | Akua T, Berezin I, Shaul O.2010. The leader intron ofAtMHX can elicit, in the absence of splicing, low-level intron-mediated enhancement that depends on the internal intron sequence. BMC Plant Biol, 10: 93-102. |
| 3 | Akua T, Shaul O.2013. TheArabidopsis thaliana MHX gene includes an intronic element that boosts translation when localized in a 5′ UTR intron. J Exp Bot, 64: 4255-4270. |
| 4 | Blom N, Gammeltoft S, Brunak S.1999. Sequence- and structure-based prediction of eukaryotic protein phosphorylation sites.J Mol Biol, 294(5): 1351-1362. |
| 5 | Chen L G, Song Y, Li S J, Zhang L P, Zou C S, Yu D Q.2012. The role of WRKY transcription factors in plant abiotic stresses.Biochim Biophys Acta, 1819(2): 120-128. |
| 6 | Ciolkowski I, Wanke D, Birkenbihl R P, Somssich I E.2008. Studies on DNA-binding selectivity of WRKY transcription factors lend structural clues into WRKY-domain function.Plant Mol Biol, 68: 81-92. |
| 7 | Eulgem T, Rushton P J, Robatzek S, Somssich I E.2000. The WRKY superfamily of plant transcription factors.Trends Plant Sci, 5(5): 199-206. |
| 8 | Gianì S, Altana A, Campanoni P, Morello L, Breviario D.2009. In transgenic rice, alpha- and beta-tubulin regulatory sequences control GUS amount and distribution through intron mediated enhancement and intron dependent spatial expression.Transgenic Res, 18(2): 151-162. |
| 9 | Gomez K A, Gomez A A.1976. Statistical Procedures for Agricultural Research with Emphasis on Rice. Los Banos, the Philippines: International Rice Research Institute. |
| 10 | Hinderhofer K, Zentgraf U.2001. Identification of a transcription factor specifically expressed at the onset of leaf senescence.Planta, 213(3): 469-473. |
| 11 | Jayaprakash T L, Ramamohan G, Krishnaprasad B T, Ganeshkumar, Prasad T G, Mathew M K, Udayakumar M.1998. Genotypic variability in differential expression oflea2 and lea3 genes and proteins in response to salinity stress in fingermillet(Eleusine coracana Gaertn) and rice, 82: 513-522. |
| 12 | Johnson C S, Kolevski B, Smyth D R.2002. TRANSPARENT TESTA GLABRA2, a trichome and seed coat development gene ofArabidopsis, encodes a WRKY transcription factor. Plant Cell, 14(6): 1359-1375. |
| 13 | Li R, Zhou J, Li D Y, Wang X M, Yang Y, Yu C L, Cheng Y, Yan C Q, Chen J P.2015. Expression ofOsWRKY7 in rice. Chin J Rice Sci, 29(6): 559-570. (in Chinese with English abstract) |
| 14 | Maeo K, Hayashi S, Kojima-Suzuki H, Morikami A, Nakamura K.2001. Role of conserved residues of the WRKY domain in the DNA-binding of tobacco WRKY family proteins.Biosci Biotech Bioch, 65(11): 2428-2436. |
| 15 | Pandey S P, Somssich I E.2009. The role of WRKY transcription factors in plant immunity.Plant Physiol, 150: 1648-1655. |
| 16 | Park S C, Kim Y H, Jeong J C, Kim C Y, Lee H S, Bang J W, Kwak S S.2011. Sweet potato late embryogenesis abundant 14 (IbLEA14) gene influences lignification and increases osmotic- and salt stress-tolerance of transgenic calli. Planta, 233(3): 621-634. |
| 17 | Popescu S C, Popescu G V, Bachan S, Zhang Z, Gerstein M, Snyder M, Dinesh-Kumar S P.2009. MAPK target networks inArabidopsis thaliana revealed using functional protein microarrays. Gene Dev, 23(1): 80-92. |
| 18 | Qiu Y P, Jing S J, Fu J, Li L, Yu D Q.2004. Cloning and analysis of expression profile of 13WRKY genes in rice. Chin Sci Bull, 49: 2159-2168. (in Chinese with English abstract) |
| 19 | Ramamoorthy R, Jiang S Y, Kumar N, Venkatesh P N, Ramachandran S.2008. A comprehensive transcriptional profiling of theWRKY gene family in rice under various abiotic and phytohormone treatments. Plant Cell Physiol, 49(6): 865-879. |
| 20 | Romero P, Obradovic Z, Dunker A K.1997. Sequence data analysis for long disordered regions prediction in the calcineurin family.Genome Inform Workshop Genome Inform, 8: 110-124. |
| 21 | Sajeevan R S, Shivanna M B, Nataraja K N.2014. An efficient protocol for total RNA isolation from healthy and stressed tissues of mulberry (Morus sp.) and other species. Am J Plant Sci, 5(13): 2057-2065. |
| 22 | Sambrook J, Fritsch E F, Maniatis T.1989. Molecular Cloning: A Laboratory Manual. 2nd ed. New York, USA: Cold Spring Harbor Laboratory Press. |
| 23 | Shen H S, Liu C T, Zhang Y, Meng X P, Zhou X, Chu C C, Wang X P.2012. OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice.Plant Mol Biol, 80(3): 241-253. |
| 24 | Singh S, Cornilescu C C, Tyler R C, Cornilescu G, Tonelli M, Lee M S, Markley J L.2005. Solution structure of a late embryogenesis abundant protein (LEA14) fromArabidopsis thaliana, a cellular stress-related protein. Protein Sci, 14(10): 2601-2609. |
| 25 | Song Y, Ai C R, Jing S J, Yu D Q.2010a. Research progress on function analysis of riceWRKY gene. Rice Sci, 17(1): 60-72. |
| 26 | Song Y, Chen L G, Zhang L P, Yu D Q.2010b. Overexpression ofOsWRKY72 gene interferes in the abscisic acid signal and auxin transport pathway of Arabidopsis. J Biosciences, 35(3): 459-471. |
| 27 | Song Y, Jing S J, Yu D Q.2009. Overexpression of the stress inducedOsWRKY08 improves the osmotic stress tolerance in Arabidopsis. Chin Sci Bull, 54: 4671-4678. (in Chinese with English abstract) |
| 28 | Uma S, Ravishankar K V, Prasad T G, Reid J L, Udaya Kumar M.1993. Abscisic acid-responsive proteins induce salinity stress tolerance in finger millet (Eleusine coracana Gaertn.) seedlings. Curr Sci, 65: 549-554. |
| 29 | Wu K L, Guo Z J, Wang H H, Li J.2005. The WRKY family of transcription factors in rice andArabidopsis and their origins. DNA Res, 12(1): 9-26. |
| 30 | Wu X L, Shiroto Y, Kishitani S, Ito Y, Toriyama K.2009. Enhanced heat and drought tolerance in transgenic rice seedlings overexpressingOsWRKY11 under the control of HSP101 promoter. Plant Cell Rep, 28(1): 21-30. |
| 31 | Xie Z, Zhang Z L, Zou X L, Huang J, Ruas P, Thompson D, Shen Q J.2005. Annotations and functional analyses of the riceWRKY gene superfamily reveal positive and negative regulators of abscisic acid signaling in aleurone cells. Plant Physiol, 137: 176-189. |
| 32 | Yamasaki K, Kigawa T, Inoue M, Watanabe S, Tateno M, Seki M, Shinozaki K, Yokoyama S.2008. Structures and evolutionary origins of plant-specific transcription factor DNA-binding domains.Plant Physiol Bioch, 46(3): 394-401. |
| 33 | (Managing Editor: Wang Caihong) |
/
| 〈 |
|
〉 |