Marker-Assisted Breeding of Thermo-Sensitive Genic Male Sterile Line 1892S for Disease Resistance and Submergence Tolerance
Received date: 2020-01-29
Accepted date: 2020-05-09
Online published: 2021-01-28
Rice line 1892S is an elite thermo-sensitive genic male sterile (TGMS) line for two-line hybrid rice production. However, 1892S is susceptible to rice blast, bacterial blight and submergence. Here we reported the introduction of blast resistance (R) gene Pi9, bacterial blight R gene Xa21 and submergence tolerance gene Sub1A into 1892S genetic background through backcrossing and marker-assisted selection. The improved TGMS line 31892S and its hybrids conferred disease resistance to rice blast and bacterial blight, and showed submergence tolerance for over 14 d without significant loss of viability. The sterility- fertility conversion of 31892S was similar to that of 1892S. 31892S and its derived hybrid rice had similar agronomic traits and grain quality with 1892S and the control hybrid rice, respectively. The newly developed 31892S provided an improved TGMS line for two-line hybrid rice production with disease resistance to rice blast and bacterial blight, and submergence tolerance with no yield penalty or change in grain quality.
Yanchang Luo, Tingchen Ma, Teo Joanne, Zhixiang Luo, Zefu Li, Jianbo Yang, Zhongchao Yin . Marker-Assisted Breeding of Thermo-Sensitive Genic Male Sterile Line 1892S for Disease Resistance and Submergence Tolerance[J]. Rice Science, 2021 , 28(1) : 89 -98 . DOI: 10.1016/j.rsci.2020.11.010
| [1] | Amante-Bordeos A, Sitch L A, Nelson R, Dalmacio R D, Oliva N P, Aswidinnoor H, Leung H. 1992. Transfer of bacterial blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice, Oryza sativa. Theor Appl Genet, 84: 345-354. |
| [2] | Brinkman M A, Frey K J. 1977. Yield-component analysis of oat isolines that produce different grain yields. Crop Sci, 17(1): 165-168. |
| [3] | Chunwongse J, Martin G B, Tanksley S D. 1993. Pre-germination genotypic screening using PCR amplification of half-seeds. Theor Appl Genet, 86(6): 694-698. |
| [4] | Cruz N D, Khush G S. 2000. Rice grain quality evaluation procedures. In: Singh R K, Singh U S, Khush G S. Aromatic Rices. New Delhi, India: Oxford and IBH Publishing Co. Pvt. Ltd: 24-36. |
| [5] | Deng H F, Shu F B, Yuan D Y. 1999. An overview of research and utilization of Annong S-1. Hybrid Rice, 14(3): 1-3. (in Chinese) |
| [6] | Duncan D B. 1955. Multiple range and multiple F tests. Biometrics, 11: 1-41. |
| [7] | Fukao T, Yeung E, Bailey-Serres J. 2011. The submergence tolerance regulator SUB1A mediates crosstalk between submergence and drought tolerance in rice. Plant Cell, 23(1): 412-427. |
| [8] | Gnanamanickam S S, Priyadarisini V B, Narayanan N N, Vasudevan P, Kavitha S. 1999. An overview of bacterial blight disease of rice and strategies for its management. Curr Sci, 77(11): 1435-1444. |
| [9] | Huang N, Angeles E R, Domingo J, Magpantay G, Singh S, Zhang G, Kumaravadivel N, Bennett J, Khush G S. 1997. Pyramiding of bacterial blight resistance genes in rice: Marker-assisted selection using RFLP and PCR. Theor Appl Genet, 95(3): 313-320. |
| [10] | Ikeda R, Khush G S, Tabien R E. 1990. A new resistance gene to bacterial blight derived from O. longistaminata. Jpn J Breeding, 40(S1): 280-281. |
| [11] | Kauffman H E, Reddy A P K, Hsieh S P Y, Merca S D. 1973. An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Rep, 57(6): 537-541. |
| [12] | Khanna A, Sharma V, Ellur R K, Shikari A B, Gopala Krishnan S, Singh U D, Prakash G, Sharma T R, Rathour R, Variar M, Prashanthi S K, Nagarajan M, Vinod K K, Bhowmick P K, Singh N K, Prabhu K V, Singh B D, Singh A K. 2015. Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice. Theor Appl Genet, 128(7): 1243-1259. |
| [13] | Khush K S, Esperanza B, Ogawa T. 1990. A new gene for resistance to bacterial blight from O. longistaminata. Rice Genet News Lett, 7: 121-122. |
| [14] | Koide Y, Ebron L A, Kato H, Tsunematsu H, Telebanco-Yanoria M J, Kobayashi N, Yokoo M, Maruyama S, Imbe T, Fukuta Y. 2011. A set of near-isogenic lines for blast resistance genes with an indica-type rainfed lowland elite rice (Oryza sativa L.) genetic background. Field Crops Res, 123(1): 19-27. |
| [15] | Liu G, Lu G, Zeng L, Wang G L. 2002. Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6. Mol Genet Genom, 267(4): 472-480. |
| [16] | Luo Y C, Ma T C, Zhang A F, Ong K H, Li Z F, Yang J B, Yin Z C. 2016. Marker-assisted breeding of the rice restorer line Wanhui 6725 for disease resistance, submergence tolerance and aromatic fragrance. Rice, 9(1): 66. |
| [17] | Luo Y C, Ma T C, Zhang A F, Ong K H, Luo Z X, Li Z F, Yang J B, Yin Z C. 2017. Marker-assisted breeding of Chinese elite rice cultivar 9311 for disease resistance to rice blast and bacterial blight and tolerance to submergence. Mol Breeding, 37(8): 106. |
| [18] | Luo Y C, Sangha J S, Wang S H, Li Z F, Yang J B, Yin Z C. 2012. Marker-assisted breeding of Xa4, Xa21 and Xa27 in the restorer lines of hybrid rice for broad-spectrum and enhanced disease resistance to bacterial blight. Mol Breeding, 30: 1601-1610. |
| [19] | Luo Y C, Yin Z C. 2013. Marker-assisted breeding of Thai fragrance rice for semi-dwarf phenotype, submergence tolerance and disease resistance to rice blast and bacterial blight. Mol Breeding, 32: 709-721. |
| [20] | Luo Y C, Zakaria S, Basyah B, Ma T C, Li Z F, Yang J B, Yin Z C. 2014. Marker-assisted breeding of Indonesia local rice variety Siputeh for semi-dwarf phonetype, good grain quality and disease resistance to bacterial blight. Rice, 7(1): 33. |
| [21] | Mackill D J, Amante M M, Vergara B S, Sarkarung S. 1993. Improved semidwarf rice lines with tolerance to submergence of seedlings. Crop Sci, 33(4): 749-753. |
| [22] | Mew T W, Alvarez A M, Leach J E, Swings J. 1993. Focus on bacterial blight of rice. Plant Dis, 77(1): 5-12. |
| [23] | Neeraja C N, Maghirang-Rodriguez R, Pamplona A, Heuer S, Collard B C Y, Septiningsih E M, Vergara G, Sanchez D, Xu K, Ismail A M, Mackill D J. 2007. A marker-assisted backcross approach for developing submergence-tolerant rice cultivars. Theor Appl Genet, 115(6): 767-776. |
| [24] | Ni D H, Song F S, Ni J L, Zhang A F, Wang C L, Zhao K J, Yang Y C, Wei P C, Yang J B, Li L. 2015. Marker-assisted selection of two-line hybrid rice for disease resistance to rice blast and bacterial blight. Field Crops Res, 184: 1-8. |
| [25] | Perez L M, Redona E D, Mendioro M S, Cruz C M V, Leung H. 2008. Introgression of Xa4, Xa7 and Xa21 for resistance to bacterial blight in thermosensitive genetic male sterile rice (Oryza sativa L.) for the development of two-line hybrids. Euphytica, 164(3): 627-636. |
| [26] | Sakthivel K, Sundaram R M, Rani N S, Balachandran S M, Neeraja C N. 2009. Genetic and molecular basis of fragrance in rice. Biotechnol Adv, 27(4): 468-473. |
| [27] | Septiningsih E M, Pamplona A M, Sanchez D L, Neeraja C N, Vergara G V, Heuer S, Ismail A M, Mackill D J. 2009. Development of submergence-tolerant rice cultivars: the Sub1 locus and beyond. Ann Bot, 103: 151-160. |
| [28] | Song F S, Ni J L, Zhang A F, Wang C L, Zhao K J, Li L, Ni D H, Yang J B. 2016. Molecular improvement of male sterile line 1892S for resistance to rice blast and bacterial blight. Mol Plant Breeding, 14(6): 1507-1515. (in Chinese with English abstract) |
| [29] | Xu J J, Wang B H, Wu Y H, Du P N, Wang J, Wang M, Yi C D, Gu M H, Liang G H. 2011. Fine mapping and candidate gene analysis of ptgms2-1, the photoperiod-thermo-sensitive genic male sterile gene in rice (Oryza sativa L.). Theor Appl Genet, 122(2): 365-372. |
| [30] | Xu K N, Mackill D J. 1996. A major locus for submergence tolerance mapped on rice chromosome 9. Mol Breeding, 2(3): 219-224. |
| [31] | Xu K N, Xu X, Fukao T, Canlas P, Maghirang-Rodriguez R, Heuer S, Ismail A M, Bailey-Serres J, Ronald P C, Mackill D J. 2006. Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice. Nature, 442: 705-708. |
| [32] | Yang L S, Bai Y S, Qian Z. 2016. Promotion and application of male sterile line 1892S breeding series resistant combination. J Anhui Agric Sci, 44(8): 63-66. (in Chinese with English abstract) |
| [33] | Yuan L P. 2014. Development of hybrid rice to ensure food security. Rice Sci, 21(1): 1-2. |
| [34] | Zhang J, Li X, Jiang G, Xu Y, He Y. 2006. Pyramiding of Xa7 and Xa21 for the improvement of disease resistance to bacterial blight in hybrid rice. Plant Breeding, 125(6): 600-605. |
| [35] | Zhou H, Zhou M, Yang Y Z, Li J, Zhu L Y, Jiang D G, Dong J F, Liu Q J, Gu L F, Zhou L Y, Feng M J, Qin P, Hu X C, Song C L, Shi J F, Song X W, Ni E D, Wu X J, Deng Q Y, Liu Z L, Chen M S, Liu Y G, Cao X F, Zhuang C X. 2014. RNase ZS1 processes UbL40 mRNAs and controls thermosensitive genic male sterility in rice. Nat Commun, 5: 4884. |
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