Cloning and Characterization of Protein Prenyltransferase Alpha Subunit in Rice
Received date: 2020-08-15
Accepted date: 2021-05-14
Online published: 2021-11-28
Protein prenylation plays a crucial role in plant development and stress response. We report the function of prenyltransferase α-subunit in rice. Protein-protein interactions showed that the farnesyl- transferase (OsPFT)/geranylgeranyltransferase-I (OsPGGT I-α) protein interacted together with OsPFT-β and OsPGGT I-β. The α- and β-subunits of OsPFT formed a heterodimer for the transfer of a farnesyl group from farnesyl pyrophosphate to the CaaX-box-containing peptide N-dansyl-GCVLS. Furthermore, the tissue expression patterns of the OsPFT and OsPGGT I subunits were similar, and these subunits were localized in the cytoplasm and nucleus. Moreover, OsPFT/OsPGGT I-α-deletion homozygous rice mutants had a lethal phenotype, and the heterozygous mutants exhibited reduced pollen viability. These results indicated that prenylation plays an important role in rice development.
Key words： protein prenylation; plant development; rice; pollen viability
Tao Wang, Lijuan Lou, Zeyu Li, Lianguang Shang, Quan Wang . Cloning and Characterization of Protein Prenyltransferase Alpha Subunit in Rice[J]. Rice Science, 2021 , 28(6) : 557 -566 . DOI: 10.1016/j.rsci.2021.05.014
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