Rice Science ›› 2023, Vol. 30 ›› Issue (5): 437-448.DOI: 10.1016/j.rsci.2023.03.015
收稿日期:
2023-01-09
接受日期:
2023-03-30
出版日期:
2023-09-28
发布日期:
2023-08-14
. [J]. Rice Science, 2023, 30(5): 437-448.
Fig. 1. Phenotypes and agronomic traits of lhd3. A, Phenotypes of wild type (WT) and mutant (lhd3) rice at the heading stage. Scale bar, 10 cm. B?I, Heading dates (B), grain lengths (C and E), grain widths (D and F), 1000-grain weights (G), plant heights (H) and panicle lengths (I) of WT and lhd3. Scale bars, 1 mm in C and D. NSD, Natural short-day; NLD, Natural long-day. Values are Mean ± SD (n = 10 in B, E, F, H and I; n = 3 in G). **, P < 0.01 compared with WT by the Student’s t-test.
Fig. 2. Cloning and expression analysis of LHD3. A, Molecular mapping of LHD3. The numbers below the lines indicate the number of recombinants. B, Distribution of SNP-index between bulked pools lhd3 and Zhonghua 11 on rice chromosome 3 (Chr. 3). Blue dot, Variant; Orange line, p99; Green line, p95; Red line, SNP-index. C, Schematic diagrams of LHD3 and mutated gene. The 1-bp deletion at 1 242 bp of LHD3 leads to premature termination of the encoded amino acid of LOC_Os03g28310. D, Phenotypes of wild type (WT), mutant lhd3 and its complementary lines. #1 and #2 represent positive transgenic lines from the complementation test. Scale bar, 20 cm. E, Expression of LHD3 in different organs by qRT-PCR analysis. LS, Leaf sheath; YP, Young panicle. Samples were harvested at natural long-day condition. The rice Ubiquitin-5 gene was used as an internal control. Values are Mean ± SD of three independent experiments and three biological replicates. F, Diurnal expression of LHD3 under controlled short-day (SD) and long-day (LD) conditions. The rice Ubiquitin-5 gene was used as an internal control. Values are Mean ± SD of three independent experiments and two biological replicates. G, Subcellular localization of LHD3 in Nicotiana benthamiana mesophyll cells. H2B-mCherry is a nucleus marker. Scale bars, 20 μm.
Fig. 3. Rhythmic expression pattern of flowering genes in wild type (WT) and mutant lhd3. A, C, E and G showed short-day conditions. B, D, F and H showed long-day conditions. The open and filled bars at the bottom represent the light and dark periods, respectively. The rice Ubiquitin-5 (UBQ5) gene was used as an internal control. Values are Mean ± SD of three independent experiments and three biological replicates.
Fig. 4. Haplotype and nucleotide diversity analysis of LHD3. A, Natural variation in LHD3 genomic region revealed 7 haplotypes in 4 702 rice accessions (Oryza sativa ssp. indica, O. sativa ssp. japonica, O. sativa ssp. aus, and intermediate). B, Haplotype analysis of LHD3. The neighbor-joining phylogenetic tree was constructed using MEGA 11 software with 1 000 bootstrap replicates (left). C, Haplotype network analysis of LHD3 in 4 702 rice accessions. Allele frequencies are proportional to the size of circles. D, Box diagram of heading date in seven haplotypes. Different lowercase letters represent significant difference at 5% level according to the Duncan’s multiple range. E, Nucleotide diversity of LHD3 in wild, japonica and indica rice. Red box marked the position of LHD3. F, Nucleotide diversity, the ratios of πwild to πindica and πwild to πjaponica surrounding LHD3.
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