Fig. 1. Function analysis of OsPIP2;2 in bakanae disease resistance of rice. A, Relative seedling lengths of wild type (WT) 9311 and Ospip2;2 mutants (Ospip2;2-1 and Ospip2;2-2) under Fusarium fujikuroi-treated conditions compared with control conditions. B, Relative seedling lengths of WT Nipponbare and its overexpression lines (OsPIP2;2-OE-1 and OsPIP2;2-OE-2) under F. fujikuroi-treated conditions compared with control conditions. C and D, Expression of OsPIP2;2 in young seedlings at the two-leaf stage (with the third leaf emerging) (C) and in various tissues in mature plants (D) determined by qRT-PCR. Expression is shown relative to that in the young shoot or stem of WT, the value of which was set to 1, with the OsActin gene used as an internal control. In A-D, data are mean ± SD (n = 15 in A and B, and n = 3 in C and D). ** indicate significant differences at the 0.01 level as determined using Student’s t-test. E, Subcellular localization of OsPIP2;2 tagged at the C terminus with green fluorescent protein (GFP) in rice protoplasts. Scale bars, 10 μm. F, Differentially expressed genes (DEGs) with P < 0.05 between Nipponbare and OsPIP2;2 overexpression lines after 12 d of normal growth upon inoculation with F. fujikuroi for 24 h using 3-day-germinated seeds. G, Comparison of DEGs between normal and F. fujikuroi treatment conditions. H and I, Kyoto Encyclopedia of Genes and Genomes