RICE SCIENCE ›› 2008, Vol. 15 ›› Issue (1): 73-76 .

• 研究简报 • 上一篇    

Response of Glutathione and Glutathione S-transferase in Rice Seedlings Exposed to Cadmium Stress

ZHANG Chun-hua 1; GE Ying 2   

  1. 1 Demonstration Laboratory of Molecular Biological Techniques, Laboratory Center of Life Science, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China; 2 College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
  • 收稿日期:2007-10-10 出版日期:2008-03-28 发布日期:2008-03-28
  • 通讯作者: GE Ying
  • 基金资助:
    the National Natural Science Foundation of China (Grant No. 30700479) and China Postdoctoral Science Foundation (Grant No. 20060390288).

摘要: A hydroponic culture experiment was done to investigate the effect of Cd stress on glutathione content (GSH) and glutathione S-transferase (GST, EC 2.5.1.18) activity in rice seedlings. The rice growth was severely inhibited when Cd level in the solution was higher than 10 mg/L. In rice shoots, GSH content and GST activity increased with the increasing Cd level, while in roots, GST was obviously inhibited by Cd treatments. Compared with shoots, the rice roots had higher GSH content and GST activity, indicating the ability of Cd detoxification was much higher in roots than in shoots. There was a significant correlation between Cd level and GSH content or GST activity, suggesting that both parameters may be used as biomarkers of Cd stress in rice.

关键词: rice, cadmium stress, glutathione, glutathione S-transferase, enzyme activity