RICE SCIENCE ›› 2006, Vol. 13 ›› Issue (4): 299-302 .

• 实验技术 • 上一篇    

A Rapid DNA Mini-prep Method for Large-Scale Rice Mutant Screening

QIU Fu-lin 1, 2, 3, WANG He-he 4, CHEN Jie 2, ZHUANG Jie-yun 2, Hei LEUNG 4, CHENG Shi-hua 2, WU Jian-li 1
  

  1. 1 Shenyang Agricultural University, Shenyang 110101, China; 2 Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 3 Rice Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110101, China; 4 Entomology and Plant Pathology Division, International Rice Research Institute, DAPO P. O. Box 7777, Metro Manila, Philippines
  • 收稿日期:2006-05-25 出版日期:2006-12-28 发布日期:2006-12-28
  • 通讯作者: CHENG Shi-hua OR WU Jian-li
  • 基金资助:
    the Department of Science and Technology, Zhejiang Province, China (Grant No.2005C4007).

摘要: A high throughput rice DNA mini-preparation method was developed. The method is suitable for large-scale mutant bank screening as well as large mapping populations with characteristics of maintaining relatively high level of DNA purity and concentration. The extracted DNA was tested and suitable for regular PCR amplification (SSR) and for Targeting Induced Local Lesion in Genome (TILLING) analysis.

关键词: rice, targeting induced local lesion in genome, DNA extraction, polymerase chain reaction