RICE SCIENCE ›› 2006, Vol. 13 ›› Issue (2): 93-98 .

• 研究报告 • 上一篇    下一篇

QTL Mapping of Low Temperature on Germination rate of Rice

CHEN Liang 1, LOU Qiao-jun 1, 2, SUN Zong-xiu 3, XING Yong-zhong 2, YU Xin-qiao 1, LUO Li-jun 1, 2   

  1. 1 Shanghai Agrobiological Gene Center, Shanghai 201106,China; 2 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 3 China National Rice Research Institute, Hangzhou 310006, China
  • 收稿日期:2005-12-21 出版日期:2006-06-28 发布日期:2006-06-28
  • 通讯作者: LUO Li-jun
  • 基金资助:
    the Youth Fund of the QIMINGXING Project of the Shanghai Committee of Science and Technology (No.04qmx1458) and Key National Basic Research Developments Program of the Ministry of Science and Technology, P. R. China (No. 2004CB117204).

摘要: To investigate the low temperature on germination capacity (LTG) a double haploid rice (DH) population with 198 lines derived from anther culture of F1 hybrid with indica line Zhenshan 97B and a perennial japonica line AAV002863 was used to construct a linkage map with 140 SSR markers. The germination rate in Zhenshan 97B and AAV002863 was 79.7% and 30.1%, while in DH population it ranged from 0 to 100% at 15℃ after 6 days. Quantitative trait loci (QTLs) controlling low temperature germinability were identified on chromosomes 3 and 10. The percentage of observed phenotypic variance attributed to qLTG-3 and qLTG-10 was 12.6% and 12.9%, respectively. Allele from Zhenshan 97B increased the LTG at qLTG-3 region, while allele from AAV002863 increased the LTG at qLTG-10 region. One pair of epistatic interaction was detected between loci on chromosomes 3 and 10. The main-effect of QTL on chromosome 10 was also involved in epistatic interaction.

关键词: rice, low temperature germinability, quantitative trait locus