A Simplified Rice DNA Extraction Protocol for PCR Analysis

摘要/Abstract

摘要： A simple protocol was established for DNA extraction using etiolated rice seedlings, whereby rice DNA was directly extracted in 0.5 mol/L NaOH solution in a single eppendorf tube. Results of comparative PCR analyses and electrophoresis showed that the DNA extracted using this method was as good and useful as that using standard CTAB method.

关键词: DNA extraction, rice, polymerase chain reaction, molecular marker, simple sequence repeats, transgene

CHEN Wen-yue , CUI Hai-rui , BAO Jin-song , ZHOU Xiang-sheng , SHU Qing-yao . A Simplified Rice DNA Extraction Protocol for PCR Analysis[J]. RICE SCIENCE, 2006, 13(1): 67-70 .

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