RICE SCIENCE ›› 2005, Vol. 12 ›› Issue (4): 255-260 .

• 研究报告 • 上一篇    下一篇

Effect of Phytosulfokine-α on Agrobacterium-Mediated Transformation in Rice

CHEN De-xi, XU Zheng-jun, MA Bing-tian, LI Shi-gui   

  1. Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
  • 收稿日期:2005-06-24 出版日期:2005-12-28 发布日期:2005-12-28
  • 通讯作者: LI Shi-gui
  • 基金资助:
    National Transgenic Plant Research and Industrialization Program (J2000-B-012), and the Excellent Doctor Paper Foundation of Ministry of Education, China (200054).

摘要: Phytosulfokine-α(PSK-α), a biologically active peptide acting as a growth factor, plays a key role in cellular differentiation and proliferation. To test if PSK-α has some influence on agrobacterium-mediated transformation in rice, PSK-α at a series of concentrations was added into co-culture medium respectively. The results showed that PSK-α indeed affected the recovery of resistant calli and the transformation frequency of rice varieties Taipei 309 and Lijiangxintuanheigu. PSK-α at the concentration of 10 nmol/L could increase induction of resistant callus and efficiency of transformation, with a 11% and 4.9% top increase, respectively than the control. However, PSK-α at 200 nmol/L could inhibit the induction of the resistant calli. Further more, the effect of PSK-αon agrobacterium-mediated transformation is related with the concentration of 2, 4-D in selection medium. Higher induction rate of resistant calli was obtained from tissues treated with PSK-α plus 2 mg/L 2, 4-D.

关键词: phytosulfokine, rice, Agrobacterium-mediated transformation