Rice Science ›› 2015, Vol. 22 ›› Issue (6): 255-263.DOI: 10.1016/S1672-6308(14)60307-3
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收稿日期:2015-04-22
接受日期:2015-06-09
出版日期:2015-06-06
发布日期:2015-09-15
. [J]. Rice Science, 2015, 22(6): 255-263.
| Gene | ID | Chromosome | Amino acid size |
|---|---|---|---|
| OsMAX1a | LOC_Os01g50530 | 1 | 412 |
| LOC_Os01g50520 | 1 | ||
| OsMAX1b | LOC_Os06g36920 | 6 | 549 |
| OsMAX1c | LOC_Os01g50590 LOC_Os01g50580 | 1 | 517 |
| OsMAX1d | LOC_Os02g12890 | 1 | 385 |
| OsMAX1e | 2 | 548 |
Table 1 OsMAX1 candidate genes in rice.
| Gene | ID | Chromosome | Amino acid size |
|---|---|---|---|
| OsMAX1a | LOC_Os01g50530 | 1 | 412 |
| LOC_Os01g50520 | 1 | ||
| OsMAX1b | LOC_Os06g36920 | 6 | 549 |
| OsMAX1c | LOC_Os01g50590 LOC_Os01g50580 | 1 | 517 |
| OsMAX1d | LOC_Os02g12890 | 1 | 385 |
| OsMAX1e | 2 | 548 |
Fig. 1. Chromosomal location and gene structure of rice OsMAX1 candidate genes.（Chr, Chromosome. Black boxes indicate exon and the connecting lines indicate intron in part B. ）
Fig. 2. Transcriptional analysis of OsMAX1.
| Marker | Sence primer | Anti-sence primer |
|---|---|---|
| RNAi-OsMAX1CON-F RNAi-OsMAX1CON-R | 5'-GCGTCGACGTTCTCAAGAGGCTTCGCTG-3' | 5'-CGAGCTCGTTCTCAAGAGGCTTCGCTG-3' |
| RNAi-OsMAX1a-F | 5'-CCCAAGCTTCAAGGTAGGGGAATTTGGTCT-3' | 5'-CGGAATTCCAAGGTAGGGGAATTTGGTCT-3' |
| RNAi-OsMAX1a-R | 5'-GCGTCGACTCCTGAAGGAAGCGATGAGA-3' | 5'-CGAGCTCTCCTGAAGGAAGCGATGAGA-3' |
| RNAi-OsMAX1e-F | 5'-CCCAAGCTTCACCTCTGGCTCTGGGAAAT-3' | 5'-CGGAATTCCACCTCTGGCTCTGGGAAAT-3' |
| RNAi-OsMAX1e-R | 5'-GCGTCGACGTCCAGCTCGCTGTCCACCA-3' | 5'-TCCCCCGGGGTCCAGCTCGCTGTCCACCA-3' |
| 5'-CCCAAGCTTGAGACGAGGTGGAAGCGCATG-3' | 5'-CGGAATTCGAGACGAGGTGGAAGCGCATG-3' |
Table 2 Sequence of RNAi markers.
| Marker | Sence primer | Anti-sence primer |
|---|---|---|
| RNAi-OsMAX1CON-F RNAi-OsMAX1CON-R | 5'-GCGTCGACGTTCTCAAGAGGCTTCGCTG-3' | 5'-CGAGCTCGTTCTCAAGAGGCTTCGCTG-3' |
| RNAi-OsMAX1a-F | 5'-CCCAAGCTTCAAGGTAGGGGAATTTGGTCT-3' | 5'-CGGAATTCCAAGGTAGGGGAATTTGGTCT-3' |
| RNAi-OsMAX1a-R | 5'-GCGTCGACTCCTGAAGGAAGCGATGAGA-3' | 5'-CGAGCTCTCCTGAAGGAAGCGATGAGA-3' |
| RNAi-OsMAX1e-F | 5'-CCCAAGCTTCACCTCTGGCTCTGGGAAAT-3' | 5'-CGGAATTCCACCTCTGGCTCTGGGAAAT-3' |
| RNAi-OsMAX1e-R | 5'-GCGTCGACGTCCAGCTCGCTGTCCACCA-3' | 5'-TCCCCCGGGGTCCAGCTCGCTGTCCACCA-3' |
| 5'-CCCAAGCTTGAGACGAGGTGGAAGCGCATG-3' | 5'-CGGAATTCGAGACGAGGTGGAAGCGCATG-3' |
Fig. 3. Over-expression of OsMAX1a and OsMAX1e in Arabidopsis max1 mutant.（A and D, Over-expression of OsMAX1a in Arabidopsis max1; B and E, Over-expression of OsMAX1e in Arabidopsis max1; C and F, Arabidopsis max1.）
Fig. 4. PCR analysis of transgenic rice plants.（M, Marker; +, Positive control; -, Negative control; Lanes 1 to 12, Transgenic rice plants.）
Fig. 5. Tillering numbers and plant heights of Nipponbare, OsMAX1CON, OsMAX1a and OsMAX1e interference in rice.（OsMAX1CON-RNAi, Conserved region interference of OsMAX1 gene. ** means significant difference at the 0.01 level. Bar represents the standard error.）
Fig. 6. Expression levels of OsMAX1CON, OsMAX1a and OsMAX1e interference offspring in rice.(** means significant difference at the 0.01 level. Bar represents the standard error.)
Fig. 7. Expression levels of OsMAX1a and OsMAX1e as affected by phosphate (P)-deficiency.(** means significant difference at the 0.01 level. Bar represents the standard error.)
Fig. 8. Expression levels of OsMAX1a and OsMAX1e after GR24, N-1-naphthylphalamic acid (NPA), indole-3-acetic acid (IAA) and kinetin (KT) treatments.(** means significant difference at the 0.01 level. Bar represents the standard error.)
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