Rice Science ›› 2018, Vol. 25 ›› Issue (1): 52-56.DOI: 10.1016/j.rsci.2017.08.004
收稿日期:2017-06-08
接受日期:2017-08-19
出版日期:2018-01-28
发布日期:2017-11-16
. [J]. Rice Science, 2018, 25(1): 52-56.
Fig. 1. Breeding scheme for MAS of Xa21. The number of plants selected for self-pollination and total numbers of transplanted plants are indicated in parentheses.MAS, Marker-assisted selection; BLB, Bacterial leaf blight.
Fig. 2. Amplification products of part BC3F2 plants with pTA248 marker that was linked to Xa21.
| Accession | 981.HUA10146 | 996.HUA10147 | 1035.HUA10153 | |||||
|---|---|---|---|---|---|---|---|---|
| Lesion length (cm) | Disease reaction | Lesion length (cm) | Disease reaction | Lesion length (cm) | Disease reaction | |||
| LT2 (CK) | 17.5 | S | 21.1 | S | 29.6 | S | ||
| IRBB21 (CK) | 2.8 | R | 3.9 | R | 16.5 | S | ||
| IR24 (CK) | 21.2 | S | 24.1 | S | 28.4 | S | ||
| BC3F2 5.1.5.1 | 4.5 | R | 7.1 | R | 11.6 | M | ||
| BC3F2 5.1.5.11 | 5.3 | R | 5.5 | R | 10.6 | M | ||
| BC3F2 5.1.5.12 | 6.8 | R | 7.6 | R | 9.8 | M | ||
| BC3F2 5.1.5.18 | 6.9 | R | 7.6 | R | 27.5 | S | ||
| BC3F2 8.5.6.21 | 6.5 | R | 6.8 | R | 19.5 | S | ||
| BC3F2 8.5.6.37 | 4.7 | R | 6.5 | R | 21.1 | S | ||
| BC3F2 8.5.6.44 | 6.2 | R | 6.7 | R | 28.3 | S | ||
| BC3F2 9.5.4.1 | 6.4 | R | 6.3 | R | 19.4 | S | ||
| BC3F2 9.5.4.23 | 4.6 | R | 4.3 | R | 21.1 | S | ||
| BC3F2 9.5.4.29 | 7.6 | R | 8 | R | 28.1 | S | ||
| BC3F2 9.5.4.43 | 4.4 | R | 4.8 | R | 14.2 | S | ||
Table 1 Bacterial leaf blight reaction of 11 BC3F2 lines carrying Xa21 in winter-spring season of 2015-2016 at Soc Trang Province, Vietnam.
| Accession | 981.HUA10146 | 996.HUA10147 | 1035.HUA10153 | |||||
|---|---|---|---|---|---|---|---|---|
| Lesion length (cm) | Disease reaction | Lesion length (cm) | Disease reaction | Lesion length (cm) | Disease reaction | |||
| LT2 (CK) | 17.5 | S | 21.1 | S | 29.6 | S | ||
| IRBB21 (CK) | 2.8 | R | 3.9 | R | 16.5 | S | ||
| IR24 (CK) | 21.2 | S | 24.1 | S | 28.4 | S | ||
| BC3F2 5.1.5.1 | 4.5 | R | 7.1 | R | 11.6 | M | ||
| BC3F2 5.1.5.11 | 5.3 | R | 5.5 | R | 10.6 | M | ||
| BC3F2 5.1.5.12 | 6.8 | R | 7.6 | R | 9.8 | M | ||
| BC3F2 5.1.5.18 | 6.9 | R | 7.6 | R | 27.5 | S | ||
| BC3F2 8.5.6.21 | 6.5 | R | 6.8 | R | 19.5 | S | ||
| BC3F2 8.5.6.37 | 4.7 | R | 6.5 | R | 21.1 | S | ||
| BC3F2 8.5.6.44 | 6.2 | R | 6.7 | R | 28.3 | S | ||
| BC3F2 9.5.4.1 | 6.4 | R | 6.3 | R | 19.4 | S | ||
| BC3F2 9.5.4.23 | 4.6 | R | 4.3 | R | 21.1 | S | ||
| BC3F2 9.5.4.29 | 7.6 | R | 8 | R | 28.1 | S | ||
| BC3F2 9.5.4.43 | 4.4 | R | 4.8 | R | 14.2 | S | ||
| Accession | 981.HUA10146 | 996.HUA10147 | 1035.HUA10153 | |||||
|---|---|---|---|---|---|---|---|---|
| Lesion length (cm) | Disease reaction | Lesion length (cm) | Disease reaction | Lesion length (cm) | Disease reaction | |||
| LT2 (CK) | 17.5 | S | 21.1 | S | 29.6 | S | ||
| IRBB21 (CK) | 2.8 | R | 3.9 | R | 11.2 | M | ||
| IR24 (CK) | 21.2 | S | 24.1 | S | 33.2 | S | ||
| BC3F3 5.1.5.1 | 5.3 | R | 6.8 | R | 14.6 | S | ||
| BC3F3 5.1.5.11 | 6.8 | R | 7.3 | R | 21 | S | ||
| BC3F3 5.1.5.12 | 3.1 | R | 4 | R | 10.8 | M | ||
| BC3F3 5.1.5.18 | 5.8 | R | 7.2 | R | 19.6 | S | ||
| BC3F3 8.5.6.21 | 7.1 | R | 8 | R | 24.1 | S | ||
| BC3F3 8.5.6.37 | 3.4 | R | 4.5 | R | 11.5 | M | ||
| BC3F3 8.5.6.44 | 5.4 | R | 6.2 | R | 12.6 | S | ||
| BC3F3 9.5.4.1 | 4.5 | R | 7.1 | R | 14.6 | S | ||
| BC3F3 9.5.4.23 | 4.2 | R | 5.7 | R | 15.1 | S | ||
| BC3F3 9.5.4.29 | 3.5 | R | 7.1 | R | 11.6 | M | ||
| BC3F3 9.5.4.43 | 4 | R | 4.7 | R | 15.1 | S | ||
Table 2 Bacterial blight reaction of 11 BC3F3 lines carrying Xa21 in spring season of 2016 at Gialam, Hanoi Province, Vietnam.
| Accession | 981.HUA10146 | 996.HUA10147 | 1035.HUA10153 | |||||
|---|---|---|---|---|---|---|---|---|
| Lesion length (cm) | Disease reaction | Lesion length (cm) | Disease reaction | Lesion length (cm) | Disease reaction | |||
| LT2 (CK) | 17.5 | S | 21.1 | S | 29.6 | S | ||
| IRBB21 (CK) | 2.8 | R | 3.9 | R | 11.2 | M | ||
| IR24 (CK) | 21.2 | S | 24.1 | S | 33.2 | S | ||
| BC3F3 5.1.5.1 | 5.3 | R | 6.8 | R | 14.6 | S | ||
| BC3F3 5.1.5.11 | 6.8 | R | 7.3 | R | 21 | S | ||
| BC3F3 5.1.5.12 | 3.1 | R | 4 | R | 10.8 | M | ||
| BC3F3 5.1.5.18 | 5.8 | R | 7.2 | R | 19.6 | S | ||
| BC3F3 8.5.6.21 | 7.1 | R | 8 | R | 24.1 | S | ||
| BC3F3 8.5.6.37 | 3.4 | R | 4.5 | R | 11.5 | M | ||
| BC3F3 8.5.6.44 | 5.4 | R | 6.2 | R | 12.6 | S | ||
| BC3F3 9.5.4.1 | 4.5 | R | 7.1 | R | 14.6 | S | ||
| BC3F3 9.5.4.23 | 4.2 | R | 5.7 | R | 15.1 | S | ||
| BC3F3 9.5.4.29 | 3.5 | R | 7.1 | R | 11.6 | M | ||
| BC3F3 9.5.4.43 | 4 | R | 4.7 | R | 15.1 | S | ||
| Accession | Growth duration (d) | Plant height (cm) | No. of tillers per plant | No. of grains per plant | 1000-grain | Polished rice rate (%) | Gelatinization temperature (ºC) | Amylose content (%) | Protein content (%) | Aroma (Score) |
|---|---|---|---|---|---|---|---|---|---|---|
| weight (g) | ||||||||||
| LT2 (CK) | 134 | 109.4 a | 7 | 885.0 a | 20.6 | 65.3 | 65 | 17.2 | 8.85 | 3 |
| BC3F3 5.1.5.1 | 134 | 111.2 a | 6.8 | 845.4 a | 21.2 | 67.4 | 65 | 17.6 | 8.82 | 3.1 |
| BC3F3 5.1.5.12 | 135 | 113.6 ab | 7.5 | 965.0 b | 21.2 | 65.6 | 65 | 17.1 | 8.91 | 3.3 |
| BC3F3 8.5.6.44 | 134 | 112.1 a | 6.5 | 872.0 a | 21.1 | 66.2 | 65 | 17.1 | 8.58 | 3.1 |
| BC3F3 9.5.4.1 | 133 | 109.6 a | 7.1 | 926.4 ab | 20.8 | 67.1 | 65 | 17.3 | 8.7 | 3.3 |
| BC3F3 9.5.4.23 | 135 | 110.3 a | 6.4 | 871.0 a | 21 | 64.9 | 65 | 16.8 | 8.92 | 3 |
Table 3 10 texted traits of five BC3F3 lines averaged from 10 individuals of each line in spring season of 2016.
| Accession | Growth duration (d) | Plant height (cm) | No. of tillers per plant | No. of grains per plant | 1000-grain | Polished rice rate (%) | Gelatinization temperature (ºC) | Amylose content (%) | Protein content (%) | Aroma (Score) |
|---|---|---|---|---|---|---|---|---|---|---|
| weight (g) | ||||||||||
| LT2 (CK) | 134 | 109.4 a | 7 | 885.0 a | 20.6 | 65.3 | 65 | 17.2 | 8.85 | 3 |
| BC3F3 5.1.5.1 | 134 | 111.2 a | 6.8 | 845.4 a | 21.2 | 67.4 | 65 | 17.6 | 8.82 | 3.1 |
| BC3F3 5.1.5.12 | 135 | 113.6 ab | 7.5 | 965.0 b | 21.2 | 65.6 | 65 | 17.1 | 8.91 | 3.3 |
| BC3F3 8.5.6.44 | 134 | 112.1 a | 6.5 | 872.0 a | 21.1 | 66.2 | 65 | 17.1 | 8.58 | 3.1 |
| BC3F3 9.5.4.1 | 133 | 109.6 a | 7.1 | 926.4 ab | 20.8 | 67.1 | 65 | 17.3 | 8.7 | 3.3 |
| BC3F3 9.5.4.23 | 135 | 110.3 a | 6.4 | 871.0 a | 21 | 64.9 | 65 | 16.8 | 8.92 | 3 |
| [1] | Adhikari T B, Mew T W, Leach J E.1999. Genotypic and pathotypic diversity inXanthomonas oryzae pv. oryzae in Nepal. Phythopathol, 89(8): 687-694. |
| [2] | Cao L Y, Zhan X D, Zhuang J Y, Cheng S H.2005. Breeding of indica hybrid rice Guodao 1 with good quality, high yield and resistance to bacterial leaf blight by marker-assisted selection technique. Hybrid Rice, 20(3): 16-18. (in Chinese with English abstract) |
| [3] | Chen S, Zhang Q F.2000. Improvement of bacterial blight resistance of hybrid rice by molecular marker-assisted selection.J Huazhong Agric Univ, 19(3): 183-189. (in Chinese with English abstract) |
| [4] | Chen S, Lin X H, Xu C G, Zhang Q F.2000. Improvement of bacterial blight resistance of ‘Minghui 63’ an elite restorer line of hybrid rice, by molecular marker-assisted selection.Crop Sci, 40: 239-244. |
| [5] | Furuya N, Taura S, Goto T, Thuy B T, Ton P H, Tsuchiya K, Yoshimura A.2012. Diversity in virulence ofXanthomonas oryzae pv. oryzae from Northern Vietnam. Jpn Agric Res Quart, 46(4): 329-338. |
| [6] | Huang N, Angeles E R, Domingo J, Magpatay G, Singh S, Zhang G, Kumaravadevil N, Bennett J, Khush G S.1997. Pyramiding of bacterial blight resistance genes in rice: Marker assisted selection using RFLP and PCR.Theor Appl Genet, 95(3): 313-320. |
| [7] | Juliano B O.1971. A simplified assay for milled-rice amylose.Cereal Sci Today, 16: 334-340. |
| [8] | Khan M A, Naeem M, Iqbal M.2014. Breeding approaches for bacterial leaf blight resistance in rice (Oryza sativa L.), current status and future directions. Eur J Plant Pathol, 139(1): 27-37. |
| [9] | Khush G S, Mackill D J, Sidhu G S.1989. Breeding rice for resistance to bacterial blight. In: IRRI. Bacterial Blight of Rice. Manila, the Philippines: International Rice Research Institute: 207-217. |
| [10] | Kibria K, Iskam M M, Begum S N.2008. Screening of aromatics rice lines by phenotypic and molecular markers.Bangl J Bot, 37: 141-147. |
| [11] | Lowry O H, Rosebrough N J, Farr A L, Randall R J.1951. Protein measurement with the folin phenol reagent.Biol Chem, 193(1): 265-275. |
| [12] | Luo Y C, Wang S H, Li C Q, Wu S, Wang D Z, Du S Y.2005. Improvement of bacterial blight resistance by molecular marker-assisted selection in a wide compatibility restorer line of hybrid rice.Chin J Rice Sci, 19(1): 36-40. (in Chinese with English abstract) |
| [13] | Mishra D, Vishnupriya M R, Anil M G, Konda K, Raj Y, Sonti R V.2013. Pathotype and genetic diversity amongst Indian isolates of Xanthomonas oryzae pv. oryzae. PLoS One, 8(11): e81996. |
| [14] | Monna L, Kitazawa N, Yoshino R, Suzuki J, Masuda H, Maehara Y, Tanji M, Sato M, Nasu S, Miobe Y.2002. Positional cloning of rice semidwarf gene, sd-1: Rice “green revolution gene” encodes a mutant enzyme involved in gibberellin synthesis. DNA Res, 9(1): 11-17. |
| [15] | NiNo-Liu D O, Ronald P C, Bogdanove A J.2006. Xanthomonas oryzae pathovars: Model pathogens of a model crop. Mol Plant Pathol, 7(5): 303-324. |
| [16] | Perez L M, Redona E D, Mendioro M S, Cruz C M V, Leung H.2008. Introgression ofXa4, Xa7 and Xa21 for resistance to bacterial blight in thermosensitive genetic male sterile rice(Oryza sativa L.) for the development of two-line hybrids. Euphytica, 164: 627-636. |
| [17] | Ronald P C, Albano B, Tabien R, Abenes L, Wu K S, McCouch S, Tankley S D.1992. Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21. Mol Gen Genet, 236(1): 113-120. |
| [18] | Singh S, Sidhu J S, Huang N, Vikal Y, Li Z, Brar D S, Dhaliwal H S, Khush G S.2001. Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into indica rice cultivar PR106. Theor Appl Genet, 102: 1011-1015. |
| [19] | Song W Y, Wang G L, Chen L L, Kim H S, Pi L Y, Gardner J, Wang B, Zhai W X, Zhu L H, Fauquet C, Ronald P C.1995. A receptor kinase-like protein encoded by the rice disease resistance geneXa27. Science, 270: 1804-1806. |
| [20] | Song W Y, Pi L Y, Wang G L, Gardner J, Holsten T, Ronald P C.1997. Evolution of the rice Xa21 disease resistance gene family. Plant Cell, 9(8): 1279-1287. |
| [21] | Tong H J, Xue Q Z.2003. II You 8220, a new disease resistant hybrid rice combination.Hybrid Rice, 18(5): 73-74. (in Chinese with English abstract) |
| [22] | Williams C E, Wang B, Holsten T E, Scambray J, de Assis Goes da Silva F, Ronald P C.1996. Markers for selection of the rice Xa21 disease resistance gene. Theor Appl Genet, 93: 1119-1122. |
| [23] | Zheng K L, Shen B, Qian H R.1991. DNA polymorphism generated by arbitrary primed PCR in rice.Rice Genet Newsl, 8: 134-136. |
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