Effects of Nitrogen Fertilizer Level on Chlorophyll Fluorescence Characteristics in Flag Leaf of Super Hybrid Rice at Late Growth Stage

doi:10.1016/S1672-6308(13)60138-9

Abstract

Abstract:

To compare the effects of slow-release nitrogen fertilizer at six different levels on the flag leaf chlorophyll fluorescence characteristics of super hybrid rice, a field fertilization experiment was conducted with super hybrid rice Y Liangyou 1 as a test material. The photosynthetic electron transport rate (ETR), effective quantum yield (EQY), photochemical quenching coefficient (qP), and non-photochemical quenching coefficient (NPQ) of flag leaves were measured at the initial heading, full heading, 10 d after full heading and 20 d after full heading stages. Results showed that the values of ETR, EQY and qP increased with rice development from initial heading to 20 d after full heading, whereas the NPQ decreased. During the measured stages, ETR, EQY and qP increased initially and then decreased as nitrogen application amount increased, but they peaked at different nitrogen fertilizer levels. The maximum ETR and EQY values appeared at the treatment of 135 kg/hm2 N. In conclusion, the optimum nitrogen amount for chlorophyll fluorescence characteristics of super hybrid rice was 135?180 kg/hm2.

Key words: super hybrid rice, slow release nitrogen fertilizer, chlorophyll fluorescence, nitrogen-saving cultivation

LONG Ji-rui, MA Guo-hui, WAN Yi-zheng, SONG Chun-fang, SUN Jian, QIN Rui-jun. Effects of Nitrogen Fertilizer Level on Chlorophyll Fluorescence Characteristics in Flag Leaf of Super Hybrid Rice at Late Growth Stage[J]. RICE SCIENCE, 2013, 20(3): 220-228.

References

Bradbury M, Bakern R. 1984. A quantitative determination of photochemical and non-photochemical quenching during the slow phase of chlorophyll fluorescence induction curve of bean leaves. Biochim Biophys Acta, 765: 275–281.
Chen Y Z, Li S S, Lin Z F. 1993. Effect of light and antioxidants on fluorescence quenching of chlorophyll in maize leaves under water stress. Acta Bot Sin, 35: 38–44. (in Chinese with English abstract)
Cheng S H, Cao L Y, Chen S G, Zhu D F, Wang X, Min S K, Zhai H Q. 2005. Conception of late-stage vigor super hybrid rice and its biological significance. Chin J Rice Sci, 19(3): 280–284. (in Chinese with English abstract)
Duan W W, Zhao H M, Guo C J, Xiao K, Li Y M. 2007. Responses of photosynthesis characteristics to nitrogen application rates in summer maize (Zea mays L.). Acta Agron Sin, 33(6): 949–954. (in Chinese with English abstract)
Genty B, Briantais J M, Baker N R. 1989. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta, 990: 87–92.
Guo T C, Feng W, Zhao H J, Xue G D, Wang H C, Wang Y H, Yao Z J. 2004. Photosynthetic characteristics of flag leaves and nitrogen effects in two winter wheat cultivars with different spike type. Acta Agron Sin, 30: 115–121. (in Chinese with English abstract)
Krause G H, Weis E. 1991. Chlorophyll fluorescence and photosynthesis: The basics. Annu Rev Plant Physiol Plant Mol Biol, 42: 313–349.
Kühl M, Chen M, Ralph P J. 2005. A niche for cyan bacteria containing chlorophyll. Nature, 433: 820.
Lazar D. 1999. Chlorophyll a fluorescence induction. Biochim Biophys Acta, 1412: 1–28.
Li F M, Ai T C, Zhou S B, Nie X J, Liu F. 2004. Influence of slow-release nitrogen fertilizers on lowland rice yield and nitrogen use efficiency. Chin J Soil Sci, 35(3): 311–315. (in Chinese with English abstract)
Li L L, Ma Z B, Xie D Y, Fang W P. 2005. Effect of different nitrogen levels on photosynthetic characteristics and yield of early-mature cotton. China Cotton, 11: 14–161. (in Chinese with English abstract)
Liu D L, Nie J, Xiao J. 2002. Study on 15N labeled rice controlled release fertilizer in increasing nitrogen utilization efficiency. Acta Laser Biol Sin, 11(2): 87–92. (in Chinese with English abstract)
Long J R, Ma G H, Zhou J, Song C F. 2007. Effects of slow release nitrogen fertilizer on the growth and development and nitrogen use efficiency of super hybrid rice Y liangyou 1. Hybrid Rice, 22(6): 48–51. (in Chinese with English abstract)
Lu Q T, Li W H, Jiang G M, Ge Q Y, Hao N B, Sun J Z, Guo R J. 2001. Studies on the characteristics of chlorophyll fluorescence of winter wheat flag leaves at different developing stages. Acta Bot Sin, 43(8): 801–804.
Luo L F, Zheng S X, Liao Y L, Nie J, Xie J, Xiang Y W. 2007. Effect of controlled release nitrogen fertilizer on protein quality of brown rice and key enzyme activity involved in nitrogen metabolism in hybrid rice. Chin J Rice Sci, 21(4): 403–410. (in Chinese with English abstract)
Platt T, Gallegos C L, Harrison W G. 1980. Photo inhibition of photosynthesis in natural assemblages of marine phytoplankton. J Marine Res, 38: 687–701.
Ralph P J, Gademann R, Dennison W C. 1998. In situ seagrass photosynthesis measured using a submersible, pulse-amplitde modulated fluorometer. Marine Biol, 132: 367–373.
Ralph P J, Macinnis-Ng C M O, Frankart C. 2005. Fluorescence imaging application: Effect of leaf age on seagrass photokinetics. Aquat Bot, 81: 69–84.
Schreiber U, Bilger W, Neubauer C. 1994. Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. In: Schulze E D, Caldwell M M. Ecophysiology of Photosynthesis. Berlin: Springer-Verlag: 49– 70.
Schreiber U, Gademann R, Ralph P J, Larkum A W D. 1997. Assessment of photosynthetic performance of Prochloron in Lissoclinum patella in hospite by chlorophyll fluorescence measurements. Plant Cell Physiol, 38: 945–951.
Shangguan Z P, Shao M A, Oyckmans J. 2000. Effect of nitrogen nutrition and water deficit on net photosynthetic rate and chlorophyll fluorescence in winter wheat. J Plant Physiol, 156: 46–51.
Shen A L, Liu C Z, Zhang F S, Huangpu X R, Kun Z L. 1997. Effects of different application rate of NPK on the growth of rice and N fertilizer utilization ratio underwater leakage and non-leakage conditions. Chin J Rice Sci, 11(4): 231–237. (in Chinese with English abstract)
Sun N X, Zong X F, Wang S G. 2005. Effects of nitrogen supply on photosynthetic traits of maize. J Southwest Agric Univ: Nat Sci, 27(2): 389–396. (in Chinese with English abstract)
Tang X R. 2000. Effect of N supply on yield and protein content and its mechanism in fodder hybrid rice. Hybrid Rice, 15(2): 34–37. (in Chinese with English abstract)
Tremkel M E. 1997. Improving Fertilizer Use Efficiency Controlled Release and Stabilized Fertilizers in Agriculture. Paris: International Fertilizer Industry Association (IFA): 99–104.
White A J, Critchley C. 1999. Rapid light curves: A new fluorescence method to assess the state of the photosynthetic apparatus. Photosynth Res, 59: 63–72.
Wu C A, Meng Q W, Zou Q, Zhao S J, Wang W. 2003. Comparative study on the photo oxidative response in different wheat cultivar leaves. Acta Agron Sin, 29(3): 339–344. (in Chinese with English abstract)
Xu D Q. 2002. Photosynthetic Efficiency. Shanghai: Shanghai Science and Technology Press: 33. (in Chinese)
Yang Q, Li Y M, Xiao K, Dou Y H. 2002. Effect of different amount of nitrogen on flag leaf senescence and yield components of wheat. J Hebei Agric Univ, 25(4): 20–24. (in Chinese with English abstract)
Zhang G D, Lu C M, Zhang Q, Bai K Z, Kuang T Y. 1997. Effects of doubled CO2 on the fluorescence induction kinetics parameters of soybean leaves grown at different nitrogen nutrition levels. Plant Nutr Fert Sci, 3(1): 24–29. (in Chinese with English abstract)
Zhang M, Shi Y X, Yang S X, Yang Y C. 2001. Status quo of study of controlled-release and slow-release fertilizers and progress made in this respect. J Chem Fert Industry, 28(5): 78–93. (in Chinese with English abstract)
Zhang S R. 1999. A discussion on chlorophyll fluorescence kinetics parameters and their significance. Chin Bull Bot, 16(4): 444–448. (in Chinese with English abstract)
Zhang W F, Gou L, Wang, Z L, Li S K, Yu S L, Cao L P. 2003. Effect of nitrogen on chlorophyll fluorescence of leaves of high-yielding cotton in Xinjiang. Sci Agric Sin, 36(8): 893–898. (in Chinese with English abstract)
Zhang X C, Shangguan Z P. 2007. Effect of nitrogen fertilization on photosynthetic pigment and fluorescence characteristics in leaves of winter wheat cultivars on dryland. J Nucl Agric Sci, 21(3): 299–304. (in Chinese with English abstract)
Zhu Z L. 2000. Loss of fertilizer N from plants-soil system and the strategies and techniques for its reduction. Soil Environm Sci, 9(1): 1–6. (in Chinese)

[1]	Debabrata PANDA*, Ramani Kumar SARKAR. Leaf Photosynthetic Activity and Antioxidant Defense Associated with Sub1 QTL in Rice Subjected to Submergence and Subsequent Re-aeration [J]. RICE SCIENCE, 2012, 19(2): 108-116.
[2]	WU Wen-ge, ZHANG Hong-cheng, QIAN Ying-fei, CHENG Ye,WU Gui-cheng, ZHAI Chao-qun, DAI Qi-gen . Analysis on Dry Matter Production Characteristics of Super Hybrid Rice [J]. RICE SCIENCE, 2008, 15(2): 110-118 .
[3]	CHEN Li-yun, XIAO Ying-hui, TANG Wen-bang, LEI Dong-yang. Practices and Prospects of Super Hybrid Rice Breeding [J]. RICE SCIENCE, 2007, 14(2): 71-77 .
[4]	WANG Jing, ZHANG Cheng-jun, CHEN Guo-xiang, WANG Ping, SHI Da-wei, LU Chuan-gen. Responses of Photosynthetic Functions to Low Temperature in Flag Leaves of Rice Genotypes at the Milky Stage [J]. RICE SCIENCE, 2006, 13(2): 113-119 .
[5]	WANG Guo-li, GUO Zhen-fei. Effects of Chilling Stress on Photosynthetic Rate and Chlorophyll Fluorescence Parameter in Seedlings of Two Rice Cultivars Differing in Cold Tolerance [J]. RICE SCIENCE, 2005, 12(3): 187-191 .
[6]	XIN Ye-yun, ZHANG Zhan, XIONG Yi-ping, YUAN Long-ping. Identification and Purity Test of Super Hybrid Rice with SSR Molecular Markers [J]. RICE SCIENCE, 2005, 12(1): 7-12 .
[7]	JIN Song-heng, WANG Pin-mei, ZHAO Kai, YANG Yi-qing, YAO Sheng, JIANG De-an. Characteristics of Gas Exchange and Chlorophyll Fluorescence in Different Position Leaves at Booting Stage in Rice Plants [J]. RICE SCIENCE, 2004, 11(5-6): 283-289 .
[8]	BAI Cheng-ke, LI Gui-shuang, PENG Chang-lian, DUAN Jun. Changes in Chlorophyll Fluorescence of Rice Mutants Induced by High Hydrostatic Pressure [J]. RICE SCIENCE, 2003, 11(1-2): 29-32 .
[9]	XU Xiao-ming, TANG Yun-lai, WANG Ying, LU Wei, DAI TXin-bin, ZHANG Rong-xian, KUANG Ting-yun. Photoinhibitive and Recovery Properties of Hybrid Rice Ⅱ You 129 under Field Conditions [J]. RICE SCIENCE, 2003, 11(1-2): 33-37 .