OsHG3 Affects Rice Palea Development, Grain Yield and Quality

doi:10.1016/j.rsci.2019.08.008

Abstract

Linjuan Ouyang, Minyu Xu, Jiang Hu, Lixin Zhu, Ting Li, Yilei Xu, Changlan Zhu, Xiaosong Peng, Xiaorong Chen, Haohua He, Jie Xu. OsHG3 Affects Rice Palea Development, Grain Yield and Quality[J]. Rice Science, 2020, 27(5): 355-358.

Figures/Tables 9

Supplemental Table 1 Primers for mapping of OsHG3.

Supplemental Table 2 Primers for real-time PCR of ORFs in mapping region.

Fig. 1. Fine mapping of OsHG3 and its functions in palea development and agronomic traits.A, Glume phenotype of two recombine inbred lines (RILs) and their parents. B, Genetic and physical maps of the region harboring OsHG3 on rice chromosome 3. The gene was mapped to a 102 kb genomic region between P6 and P7, including 12 open reading frames (ORFs). C and D, Grain shape of 9311 and NIL-OsHG3, respectively. Scale bars, 5 mm. E and F, Lemma and palea development in 9311 and NIL plants. Scale bars, 4 mm. G and H, Lateral marginal regions of paleas in 9311 (G) and NIL-2 (H). The arrows are vasular bundles. Scale bars, 100 μm. I, J, K, L and M, Yield and quality traits of 9311 and NILs. I, Grain width. J, Seed-setting rate. K, Yield per plant. L and M, Breakdown value (BDV) and setback value (SBV) from rapid viscosity analyzer. N, Expression of the 12 ORFs in palea and lemma of 9311. Actin1 was used as the internal control. Data are Mean ± SD (n = 3). * and **, Significances at the 0.05 and 0.01 levels, respectively, by the Student’s t-test.

Supplemental Fig. 1. Glume phenotype of F1 from cross of BC37 and NPB.

Supplemental Table 3 Segregation analysis of the OsHG3 allele in several F2 populations.

Supplemental Table 4 12 predicated ORFs and their annotations in the OsHG3 on rice chromosome 3.

Supplemental Fig. 2. Development of nearly isogenic lines.A and B, Phenotype of NIL-OsHG3 before and after heading. Scale bars, 5 cm. C, Graphical genotype of NIL-OsHG3 in 9311 background.

Supplemental Fig. 3. Phenotype of NIL-OsHG3.A, B and C, Paraffin section analysis of lemma and palea at 9311 and NILs; D, E, F and G, Several agronomic traits of 9311 and NILs. D, Tillering number; E, Grains per panicle; F, Grain length; G, 1000-grain weight. Data are Mean ± SD (n = 3). *, P < 0.05; **, P < 0.01 (Student’ s t-test using SPSS Statistics software).

Supplemental Fig. 4. Expression pattern of genes in the region harboring OsHG3.A and B, comparison the expression of total 12 ORFs in palea and lemma between 9311 and NILs.

References 21

[1]	Abebe T, Skadsen R W, Kaeppler H F.2004. Cloning and identification of highly expressed genes in barley lemma and palea. Crop Sci, 44: 942-950.
[2]	Cheabu S, Panichawong N, Rattanametta P, W B, Kasemsap P, Arikit S, Vanavichit A, Malumpong C.2019. Screening for spikelet fertility and validation of heat tolerance in a large rice mutant population. Rice Sci, 26(4): 229-238.
[3]	Dai Z Y, Wang J, Zhu M L, Miao X X, Shi Z Y.2016. OsMADS1 represses microRNA172 in elongation of palea/lemma development in rice. Front Plant Sci, 7: 1891.
[4]	Gao Z Y, Zhao S C, He W M, Guo L B, Peng Y L, Wang J J, Guo X S, Zhang X M, Rao Y C, Zhang C, Dong G J, Zheng F Y, Lu C X, Zhou Q, Liu H J, Wu H Y, Xu J, Ni P X, Zeng D L, Liu D H, Tian P, Gong L H, Ye C, Zhang G H, Wang J, Tian F K, Xue D W, Liao Y, Zhu L, Chen M S, Li J Y, Cheng S H, Zhang G Y, Wang J, Qian Q.2013. Dissecting yield-associated loci in super hybrid rice by resequencing recombinant inbred lines and improving parental genome sequences. Proc Natl Acad Sci USA, 110: 14492-14497.
[5]	Gille S, Pauly M.2012. O-acetylation of plant cell wall poly- saccharides. Front Plant Sci, 31: 12.
[6]	Immink R G, Kaufmann K, Angenent G C.2010. The ‘ABC’ of MADS domain protein behaviour and interactions. Semin Cell Dev Biol, 21(1): 87-93.
[7]	Lombardo F, Yoshida H.2015. Interpreting lemma and palea homologies: A point of view from rice floral mutants. Front Plant Sci, 6: 61.
[8]	Luo Q, Zhu L H.2002. Progress of molecular floral development research in rice. Hereditas, 24(1): 87-93.
[9]	Manabe Y, Nafisi M, Verhertbruggen Y, Orfila C, Gille S, Rautengarten C, Cherk C, Marcus S E, Somerville S, Pauly M, Knox J P, Sakuragi Y, Scheller H V.2011. Loss-of-function mutation of REDUCED WALL ACETYLATION2 in Arabidopsis leads to reduced cell wall acetylation and increased resistance to Botrytis cinerea. Plant Physiol, 155(3): 1068-1078.
[10]	Manabe Y, Verhertbruggen Y, Gille S, Harholt J, Chong S L, Pawar P M A, Mellerowicz E J, Tenkanen M, Cheng K, Pauly M, Scheller H V.2013. Reduced wall acetylation proteins play vital and distinct roles in cell wall o-acetylation in Arabidopsis. Plant Physiol, 163(3): 1107-1117.
[11]	Meng Q C, Li X F, Zhu W W, Yang L, Liang W Q, Dreni L, Zhang D B.2017. Regulatory network and genetic interactions established by OsMADS34 in rice inflorescence and spikelet morphogenesis. J Integr Plant Biol, 59(9): 693-707.
[12]	Nafisi M, Stranne M, Fimognari L, Atwell S, Martens H J, Pedas P R, Hansen S F, Nawrath C, Scheller H V, Kliebenstein D J, Sakuragi Y.2015. Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses. Front Plant Sci, 6: 550.
[13]	Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, Nagato Y.2003. SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice. Development, 130(4): 705-718.
[14]	Pan X W, Liu W Q, Li Y C, Xiong H B, Sheng X N, Duan Y H, Yu Y Y, Zhao W J, Wei X C, Li X X.2019. Identification and genetic analysis of split husk mutant sh1 in rice. Chin J Rice Sci, 33(4): 323-330. (in Chinese with English abstract)
[15]	Pawar P M A, Ratke C, Balasubramanian V K, Chong S L, Gandla M L, Adrissola M, Sparrman T, Hedenström M, Szwaj K, Derba-Maceluch M, Gaertner C, Mouille G, Ezcurra I, Tenkanen M, Jönsson L J, Mellerowicz E J.2017. Downregulation of RWA genes in hybrid aspen affects xylan acetylation and wood saccharification. New Phytol, 214(4): 1497-1505.
[16]	Sang X C, Li Y F, Luo Z K, Ren D Y, Fang L K, Wang N, Zhao F M, Ling Y H, Yang Z L, Liu Y S, He G H.2012. CHIMERIC FLORAL ORGANS1, encoding a monocot-specific MADS box protein, regulates floral organ identity in rice. Plant Physiol, 160(2): 788-807.
[17]	Sui J M, Li X, Yan S, Yan C J, Zhang R, Tang S Z, Lu J F, Chen Z X, Gu M H.2005. Studies on the rice RVA profile characteristics and its correlation with the quality. Sci Agric Sin, 38(4): 657-663.
[18]	Tao J H, Liang W Q, An G, Zhang D B.2018. OsMADS6 controls flower development by activating rice FACTOR OF DNA METHYLATION LIKE1. Plant Physiol, 177(2): 713-727.
[19]	Toriba T, Hirano H Y.2014. The DROOPING LEAF and OsETTIN2 genes promote awn development in rice. Plant J, 77(4): 616-626.
[20]	Yan D W, Zhang X M, Zhang L, Ye S H, Liu J Y, Li Q, He Z H.2015. CURVED CHIMERIC PALEA 1 encoding an EMF1-like protein maintains epigenetic repression of OsMADS58 in rice palea development. Plant J, 82(1): 12-24.
[21]	Yuan Y X, Teng Q, Zhong R Q, Ye Z H.2013. The Arabidopsis DUF231 domain-containing protein ESK1 mediates 2-O and 3-O-acetylation of xylosyl residues in xylan. Plant Cell Physiol, 54(7): 1186-1199.