Genetic Relationship and Structure Analysis of Root Growth Angle for Improvement of Drought Avoidance in Early and Mid-Early Maturing Rice Genotypes

doi:10.1016/j.rsci.2020.01.003

Abstract

Abstract:

Deeper rooting 1 (Dro1) and Deeper rooting 2 (Dro2) are the QTLs that contribute considerably to root growth angle assisting in deeper rooting of rice plant. In the present study, a set of 348 genotypes were shortlisted from rice germplasm based on root angle study. Screening results of the germplasm lines under drought stress identified 25 drought tolerant donor lines based on leaf rolling, leaf drying, spikelet fertility and single plant yield. A panel containing 101 genotypes was constituted based on screening results and genotyped using Dro1 and Dro2 markers. Structure software categorized the genotypes into four sub-populations with different fixation index values for root growth angle. The clustering analysis and principal coordinate analysis could differentiate the genotypes with or without deeper rooting trait. The dendrogram constructed based on the molecular screening for deep rooting QTLs showed clear distinction between the rainfed upland cultivars and irrigated genotypes. Eleven genotypes, namely Dular, Tepiboro, Surjamukhi, Bamawpyan, N22, Dinorado, Karni, Kusuma, Bowdel, Lalsankari and Laxmikajal, possessed both the QTLs, whereas 67 genotypes possessed only Dro1. The average angle of Dro positive genotypes ranged from 82.7º to 89.7º. These genotypes possessing the deeper rooting QTLs can be taken as donor lines to be used in marker-assisted breeding programs.

Key words: root growth angle, Dro1, Dro2, deeper rooting, upland rice, drought avoidance

Pandit Elssa, Kumar Panda Rajendra, Sahoo Auromeera, Ranjan Pani Dipti, Kumar Pradhan Sharat. Genetic Relationship and Structure Analysis of Root Growth Angle for Improvement of Drought Avoidance in Early and Mid-Early Maturing Rice Genotypes[J]. Rice Science, 2020, 27(2): 124-132.

Figures/Tables 9

References 37

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Name	Forward (5'-3')	Reverse (5'-3')	Gene	Reference
RM6089	CCACCGAATCGAATAACCAC	ATGGCCAGCGTGATCTCC	Dro2	Uga et al.(2013)
ID07_14	CCATGATCAAACCACACAGC	TGTCAGGGCACCATGACTTA	Dro1	Uga et al.(2008)
ID12_17	ACCGTAGCGTTAGCATGGAC	ACTACGAGAATGCGGTGCTT	Dro1	Uga et al.(2008)
RM24393	TAGCTGCTTAGCTTTGACTTGG	ATGTAATCCTACGAGGAGATCG	Dro1	Uga et al. (2010)
RM7424	AGAAGCCCATCTAGCAGCAG	TCAAGCTAGCCACACAGCTG	Dro1	Uga et al.(2010)
SNP02-KP	GTCTAGATCACG-CAGTGAAT	TCGCATGATGATGACCAAGT	Dro1	Uga et al.(2013)
SNP02-IR64	ATCGTCTAGATCACGCATGAAC	AGGGTGGCTTTACCTCCGTA	Dro1	Uga et al.(2013)
Dro1-CAPS5	GCACAAGATGGGAGGAGAGT	CATGGGTGAGAATCGTGTTG	Dro1	Uga et al.(2013)
Dro1-INDEL	GCAGACGCTCGTAACACGTA	GT-GGCAGCTCCATCAACTCT	Dro1	Uga et al.(2013)

Name	Forward (5'-3')	Reverse (5'-3')	Gene	Reference
RM6089	CCACCGAATCGAATAACCAC	ATGGCCAGCGTGATCTCC	Dro2	Uga et al.(2013)
ID07_14	CCATGATCAAACCACACAGC	TGTCAGGGCACCATGACTTA	Dro1	Uga et al.(2008)
ID12_17	ACCGTAGCGTTAGCATGGAC	ACTACGAGAATGCGGTGCTT	Dro1	Uga et al.(2008)
RM24393	TAGCTGCTTAGCTTTGACTTGG	ATGTAATCCTACGAGGAGATCG	Dro1	Uga et al. (2010)
RM7424	AGAAGCCCATCTAGCAGCAG	TCAAGCTAGCCACACAGCTG	Dro1	Uga et al.(2010)
SNP02-KP	GTCTAGATCACG-CAGTGAAT	TCGCATGATGATGACCAAGT	Dro1	Uga et al.(2013)
SNP02-IR64	ATCGTCTAGATCACGCATGAAC	AGGGTGGCTTTACCTCCGTA	Dro1	Uga et al.(2013)
Dro1-CAPS5	GCACAAGATGGGAGGAGAGT	CATGGGTGAGAATCGTGTTG	Dro1	Uga et al.(2013)
Dro1-INDEL	GCAGACGCTCGTAACACGTA	GT-GGCAGCTCCATCAACTCT	Dro1	Uga et al.(2013)