A New PCR/LDR-Based Multiplex Functional Molecular Marker for Marker-Assisted Breeding in Rice

doi:10.1016/j.rsci.2020.11.002

Abstract

Huangwei Chu, Rongjian Tu, Fuan Niu, Jihua Zhou, Bin Sun, Zhongyong Luo, Can Cheng, Liming Cao. A New PCR/LDR-Based Multiplex Functional Molecular Marker for Marker-Assisted Breeding in Rice[J]. Rice Science, 2021, 28(1): 6-10.

Figures/Tables 1

Fig. 1. Genotype analysis of five elite rice varieties using multiplex functional marker (FM).A, Genotype analysis of five elite rice varieties using the multiplex FM. Ligase detection reaction (LDR) products differing in length are generated based on variant lengths of probes (Table S4). For NRT1.1Bind (+/-), the LDR products are 77 nt (+) and 79 nt (-). For COLD1jap (+/-), the LDR products are 86 nt (+) and 81 nt (-). For badh2-E7 (+/-), the LDR products are 91 nt (+) and 89 nt (-). For OgTT1 (+/-), the LDR products are 93 nt (+) and 95 nt (-). For Wx-mq (+/-), the LDR products are 97 nt (+) and 99 nt (-).B, Genotype analysis of three independent F1 lines using the multiplex FM. F1-1 is heterozygous in NRT1.1Bind and COLD1jap loci, F1-2 is heterozygous in OgTT1 locus, and F1-3 is heterozygous in badh2-E7 and Wx-mq loci.

References 35

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