Rice Science ›› 2023, Vol. 30 ›› Issue (1): 6-10.DOI: 10.1016/j.rsci.2022.03.003
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Tan Yanping1,#, Deng Shiqi1,#, Qin Yonghua1, Xu Xin1, Yu You1, Cui Liu1, Wang Chuntai1, Jiang Changjie2,3(
), Liu Xinqiong1()
Received:2022-01-17
Accepted:2022-03-30
Online:2023-01-28
Published:2022-11-11
Contact:
Jiang Changjie, Liu Xinqiong
About author:First author contact:#These authors contributed equally to this work
This is an open access article under the CC BY-NC-ND license (
Peer review under responsibility of China National Rice Research Institute
Tan Yanping, Deng Shiqi, Qin Yonghua, Xu Xin, Yu You, Cui Liu, Wang Chuntai, Jiang Changjie, Liu Xinqiong. Evaluation of Medicinal Plant Extracts for Rice Blast Disease Control[J]. Rice Science, 2023, 30(1): 6-10.
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| Plant | Extraction solvent | Average inhibition rate (%) | Remark |
| Negative control | Ethanol (10%) | 10 ± 2 | Most spores germinated and produced long germ tubes (Fig. S1-A) |
| Positive control | Isoprothiolane (20 μg/mL) | 100 ± 0** | Spore germination was almost completely inhibited (Fig. S1-B) |
| Paris polyphylla | Ethanol | 100 ± 0** | Spore germination was almost completely inhibited (Fig. S1-C) |
| Seed | 52 ± 4** | ||
| Stem | 43 ± 5** | ||
| Leaf | 51 ± 5** | ||
| Pericarp | 77 ± 7** | ||
| Root | 78 ± 8** | ||
| Swertia bimaculate | n-butanol | 15 ± 2 | Most spores germinated and produced long germ tubes |
| Petroleum ether | 15 ± 2 | ||
| Ethyl acetate | 15 ± 2 | ||
| Menispermum dauricum | Ethyl acetate | 20 ± 2** | Low inhibition rates; long germ tubes were observed |
| Water | 20 ± 2** | ||
| n-butanol | 50 ± 5** | Spore germination was highly inhibited (Fig. S1-D) | |
| Nelumbo nucifera | n-butanol | 20 ± 3* | Low inhibition rates; long germ tubes were observed |
| Petroleum ether | 20 ± 3* | ||
| Chloroform | 20 ± 3* | ||
| Phellodendron chinense | Ethyl acetate | 70 ± 5** | Spore germination was mostly inhibited |
| Water | 10 ± 2 | Most spores germinated and produced long germ tubes | |
| Carthamus tinctorius | n-butanol | 20 ± 3* | Low inhibition rates; long germ tubes were observed |
| Water | 20 ± 3* | ||
| Taraxacum mongolicum | Petroleum ether | 20 ± 4* | Low inhibition rates; long germ tubes were observed |
| Water | 20 ± 4* | ||
| Momordica charantia | Petroleum ether | 20 ± 2** | Low inhibition rates; long germ tubes were observed |
| n-butanol | 20 ± 2** | ||
| Ethyl acetate | 20 ± 2** | ||
| Chloroform | 30 ± 2** | Moderate inhibition rate; long germ tubes were observed | |
| Twenty extracts with ≥ 10% inhibition rates are listed. Data are Mean ± SD. * and **, Statistically significant at P < 0.05 and P < 0.01, respectively, in Dunnett’s multiple comparison test with the negative control (10% ethanol). | |||
Table 1. Inhibitory effects of 20 plant extracts and ethanol extracts from different parts of Paris polyphylla on Magnaporthe oryzae sporegermination.
| Plant | Extraction solvent | Average inhibition rate (%) | Remark |
| Negative control | Ethanol (10%) | 10 ± 2 | Most spores germinated and produced long germ tubes (Fig. S1-A) |
| Positive control | Isoprothiolane (20 μg/mL) | 100 ± 0** | Spore germination was almost completely inhibited (Fig. S1-B) |
| Paris polyphylla | Ethanol | 100 ± 0** | Spore germination was almost completely inhibited (Fig. S1-C) |
| Seed | 52 ± 4** | ||
| Stem | 43 ± 5** | ||
| Leaf | 51 ± 5** | ||
| Pericarp | 77 ± 7** | ||
| Root | 78 ± 8** | ||
| Swertia bimaculate | n-butanol | 15 ± 2 | Most spores germinated and produced long germ tubes |
| Petroleum ether | 15 ± 2 | ||
| Ethyl acetate | 15 ± 2 | ||
| Menispermum dauricum | Ethyl acetate | 20 ± 2** | Low inhibition rates; long germ tubes were observed |
| Water | 20 ± 2** | ||
| n-butanol | 50 ± 5** | Spore germination was highly inhibited (Fig. S1-D) | |
| Nelumbo nucifera | n-butanol | 20 ± 3* | Low inhibition rates; long germ tubes were observed |
| Petroleum ether | 20 ± 3* | ||
| Chloroform | 20 ± 3* | ||
| Phellodendron chinense | Ethyl acetate | 70 ± 5** | Spore germination was mostly inhibited |
| Water | 10 ± 2 | Most spores germinated and produced long germ tubes | |
| Carthamus tinctorius | n-butanol | 20 ± 3* | Low inhibition rates; long germ tubes were observed |
| Water | 20 ± 3* | ||
| Taraxacum mongolicum | Petroleum ether | 20 ± 4* | Low inhibition rates; long germ tubes were observed |
| Water | 20 ± 4* | ||
| Momordica charantia | Petroleum ether | 20 ± 2** | Low inhibition rates; long germ tubes were observed |
| n-butanol | 20 ± 2** | ||
| Ethyl acetate | 20 ± 2** | ||
| Chloroform | 30 ± 2** | Moderate inhibition rate; long germ tubes were observed | |
| Twenty extracts with ≥ 10% inhibition rates are listed. Data are Mean ± SD. * and **, Statistically significant at P < 0.05 and P < 0.01, respectively, in Dunnett’s multiple comparison test with the negative control (10% ethanol). | |||
Fig. 1. Antifungal and resistance-inducing activities against rice blast fungus Magnaporthe oryzae. A, Increase in blast resistance of rice seedlings by application of Paris polyphylla pericarp extract. Four-leaf stage rice seedlings were foliar sprayed with P. polyphylla pericarp extract, followed by blast inoculation at 24 h later. Relative fungal growth of M. oryzae was examined after 7 d of blast inoculation. B, Increase in expression levels of rice defense genes (WRKY45, PR1b and PBZ1) by P. polyphylla pericarp extract. Gene expression was examined after 3 d of blast inoculation. Data are Mean ± SD (n = 12). * indicates significant difference from the mock-treated plants (t-test, P < 0.05).
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