Effect of GW8 Gene Editing on Appearance Quality of Erect-Panicle Type (dep1) Japonica Rice

doi:10.1016/j.rsci.2022.12.002

Abstract

Mao Ting, Chen Hongfa, Li Xin, Liu Yan, Zhong Shuncheng, Wang Shiyu, Zhao Yizhou, Zhang Zhan, Ni Shanjun, Huang He, Li Xu, Hu Shikai. Effect of GW8 Gene Editing on Appearance Quality of Erect-Panicle Type (dep1) Japonica Rice[J]. Rice Science, 2023, 30(5): 359-363.

Figures/Tables 2

Fig. 1. Comparison of grain shape of near-isogenic lines (NILs) and analysis of relevant influencing factors. A and B, Performances of grain length (A) and grain width (B) of NILs. Scale bars, 5 mm. C?E, Comparisons of grain length (C), grain width (D) and grain length to width ratio (E) of NILs. F?H, Comparisons of glume epidermal cell size (F), cell length (G) and cell width (H) of NILs. Scale bar, 10 μm. I?K, Comparisons of the levels of indole-3-acetic acid (IAA) (I), gibberellin A3 (GA3) (J) and brassinolide (BR) (K) in developing glumes of NILs. Data are Mean ± SD (n = 9). Different lowercase letters above the bars indicate significant differences at P < 0.05 determined by the Student- Newman-Keuls test.

Fig. 2. Comparison of chalkiness degree of near-isogenic lines (NILs) and analysis of releted influencing factors. A and B, Comparisons of chalkiness degree in endosperms of NILs. Scale bar, 5 mm. C, Average grain filling rate of NILs. D?G, Comparisons of enzyme activities of adenosine diphosphate glucose (ADPG) (D), granule-bound starch synthase (GBSS) (E), starch branching enzymes (SBE) (F) and soluble starch synthase (SSS) (G) in endosperms of NILs. H?L, Comparisons of hormone levels of indole-3-acetic acid (IAA) (H), abscisic acid (ABA) (I), cytokinins (CTK) (J), zeatin (ZT) (K) and zeatin riboside (ZR) (L) in endosperms of NILs. Data are Mean ± SD (n = 9). Different lowercase letters above the bars indicate significant differences at P < 0.05 determined by the Student- Newman-Keuls test.

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