Oriented Generation of Novel Thermo-Sensitive Genic Male Sterile Lines with Improved Grain Shape and Outcrossing Rate in Early-Season Rice

doi:10.1016/j.rsci.2023.11.006

Abstract

Zhang Huali, Chen Junyu, Li Ruiqing, Wang Huimei, Dai Dongqing, Liang Minmin, Wu Mingyue, Ma Liangyong. Oriented Generation of Novel Thermo-Sensitive Genic Male Sterile Lines with Improved Grain Shape and Outcrossing Rate in Early-Season Rice[J]. Rice Science, 2024, 31(2): 129-133.

Figures/Tables 1

Fig. 1. Orientation mutagenesis of TMS5 and GS3 genes develops thermo-sensitive genic male sterile lines with improved grain quality and outcrossing rate in early-season india rice. A, Schematic diagram of the targeted sites of TMS5 and GS3 genes and two gs3tms5 mutation types in each variety. In wild type (WT), the targeted sites are highlighted in blue and the protospacer adjacent motif (PAM) sequences are highlighted in green. Untranslated regions, exons, and introns are indicated by blue rectangles, green rectangles, and lines, respectively. In mutants, the targeting sequences are highlighted in blue and the PAM sequences in green. Mutations with insertions are in red lowercase letters. The deleted sequences are shown by red dashed lines. B-E, Comparison of grain length (GL, B), ratio of grain length to grain width (L/W, C), 1000-grain weight (TGW, D), and grain shape (E) between WT and mutant lines. Scale bars in (E) are 1 cm. F, Plant stature of the WT and gs3tms5 mutant lines at the grain-filling stage during the normal rice growing season. Scale bars are 10 cm. G and H, Anther pollen grains stained with I2-KI under high temperature (G, 30 ºC in the normal field) and low temperature (H, 22 ºC at a cold water pool) at the heading stage. Scale bars in the above and below are 1 mm and 100 μm, respectively. I, Spikelet of each genotype at the flowering stage. Scale bars are 2 mm. J, Comparison of stigma exsertion rate. K, Comparison of appearance of polished rice grains. Scale bars are 1 cm. L-N, Comparison of chalky grain rate (L), amylose content (M), and gel consistency (N). In B-D, J, and L-N, data are Mean ± SD (n = 5). * and ** represent significant differences at the 0.05 and 0.01 levels, respectively, by the t-test.

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