Fig. 1. Trimethylsilyl phenylacetate (T-phe) effectively antagonizes pathogenicity of Magnaporthe oryzae and promotes rice growth. A, Chemical structure of T-phe. B and C, Colony morphology (B) and colony diameter statistical analysis (C) of M. oryzae under different concentrations of T-phe. CK, Control group. Scale bar, 1 cm. D and E, Pathogenicity of different concentrations of T-phe on barley (D) and rice (E) by pot spray after conidia treatment. Scale bars, 1 cm. F, Rice leaf lesion areaquantified using ImageJ. G, Fungal biomass in CK and T-phe treated-infected rice leaves was quantified using DNA-based qPCR. The infected rice leaves were harvested at 4 d post-inoculation. H, Differential expression volcano plot between T-phe treatment group and CK group. The horizontal coordinate (log2FC) reflects the magnitude and direction of expression changes, while the vertical coordinate [-log10(Q-value)] indicates the statistical significance of differential expression. FC, Fold change. I, Comparison of colony growth of Sclerotium rolfsii, Fusarium graminearum, Bipolaris maydis, Colletotrichum scovillei, and Phytophthora capsici between the CK and T-phe treatment group. Scale bar, 1 cm. J−L, Phenotypes of germinating seeds (J), bud length (K), and root length (L) of rice seeds treated with T-phe. Scale bars, 1 cm. M−O, Plant height and root length of different rice variety seedlings treated with 200 μg/mL of T-phe after 14 d of transplantation. Scale bars, 1 cm. X3, Xizi 3; N18, Nongxiang 18. Data are presented as mean ± SD (n = 3). The comparison between the T-phe group and CK group was conducted using the independent sample t-test in G; the comparisons between multiple T-phe groups and CK groupwere performed using one-way analysis of variance (ANOVA), followed by Duncan’s multiple comparison test in C, F, K, L, N, O. *, P < 0.05; **, P < 0.01.