Optimizing Anther Culture for Doubled Haploid Breeding of Rice Using Yongyou 1540

doi:10.1016/j.rsci.2025.08.011

Rice Science ›› 2026, Vol. 33 ›› Issue (1): 5-8.DOI: 10.1016/j.rsci.2025.08.011

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Optimizing Anther Culture for Doubled Haploid Breeding of Rice Using Yongyou 1540

Gan Yan¹^,³, Xiao Kailing¹^,³, Lin Jialing², Hong Yifei², Li Chuyi², Yu Hang¹^,³, Zhang Long²^,³, Cui Shiyong¹^,³, Sun Yanbo⁴^,⁵, Li Jinying⁴^,⁵, Chen Mingjiao³, Chu Huangwei⁶, Lin Wenxiong¹^,³, Yang Xuelian¹^,³(), Wang Wenfei¹^,³()

¹College of JunCao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
²College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
³Agricultural Ecology Research Institute, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
⁴Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou 350002, China
⁵Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou 350002, China
⁶Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

Received:2025-06-16 Accepted:2025-08-21 Online:2026-01-28 Published:2026-02-03
Contact: Wang Wenfei (wenfeiwang@fafu.edu.cn); Yang Xuelian (xlyang@fafu.edu.cn)

Abstract

Gan Yan, Xiao Kailing, Lin Jialing, Hong Yifei, Li Chuyi, Yu Hang, Zhang Long, Cui Shiyong, Sun Yanbo, Li Jinying, Chen Mingjiao, Chu Huangwei, Lin Wenxiong, Yang Xuelian, Wang Wenfei. Optimizing Anther Culture for Doubled Haploid Breeding of Rice Using Yongyou 1540[J]. Rice Science, 2026, 33(1): 5-8.

Figures/Tables 1

Fig. 1. Anther culture process of Yongyou 1540 (YY1540) and Hanyou 73 (HY73). A, Anthers on induction culture medium. Scale bar, 2 cm. B, Anthers producing calli at approximately 40 d post-culture. Scale bar, 1 mm. C, Calli showing green spots. Scale bar, 1 mm. D, Green shoots differentiated from calli. Scale bar, 2 mm. E, Albino shoots formed from calli. Scale bar, 1 cm. F, Effect of temperatures on shoot regeneration. Data represent mean ± SD (n > 100). Different lowercase letters in the same column for the same variety indicate significant differences between treatments (P < 0.05). G, Green plantlets regenerated from calli. Scale bar, 2 cm. H, Adventitious root formation. Scale bar, 1 cm. I, Acclimatization of regenerated plantlets in a greenhouse. Scale bar, 2 cm. J, Regenerated plants in the field. Scale bar, 10 cm. K, PCR screening using insertion/deletion (InDel) markers spanning 12 chromosomes (Chr.). Y, YY1540 F1 plants; Lanes 1-19, Regenerated doubled haploid plants. L and M, Ploidy analysis of diploid (seedling #35, L) and haploid (seedling #55, M) regenerated plants by flow cytometry. Histograms show relative nuclear DNA content.

References 17

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Optimizing Anther Culture for Doubled Haploid Breeding of Rice Using Yongyou 1540

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