RICE SCIENCE

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Recovery and Biological Properties of Nitrate Non-utilizing Mutants of Rice Blast, Magnaporthe grisea

ZHANG Chuan-qing, ZHOU Ming-guo   

  1. Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture; Nanjing Agricultural University, Nanjing 210095, China
  • Received:2003-12-23 Revised:1900-01-01 Online:2004-06-30 Published:2004-06-30

Abstract:

Eleven nitrate non-utilizing (nit) mutants were recovered from six isolates of Magnaporthe grisea cultured on MM media amended with 60 g/L potassium chlorate, with a frequency of 1.42 %. Some biological properties, such as growth rate, growth biomass, cultural characters, conidial production, sexual reproduction ability, and pathogenicity were compared between nit mutants and their parent isolates. Results showed that all the nit mutants were resistant to chlorate. Some important biological properties such as the growth rate on YPSA, conidial production ability on TPSA, pathogenicity, had no significant differences between nit mutants and their parent isolates. Mating type didn’t change, but perithecia production ability of fertile isolates changed significantly as compared with that of their parent isolates. Therefore, the nit can be used as a genetic marker to study the genetics such as pathogenicity, fungicide resistance in Magnaporthe grisea.

Key words: Magnaporthe grisea, nitrate non-utilizing mutant, biological property, genetic marker