RICE SCIENCE ›› 2010, Vol. 17 ›› Issue (3): 185-191 .DOI: 10.1016/S1672-6308(09)60016-0

• Research Paper • Previous Articles     Next Articles

Comparative Analysis on Genomes from Oryza alta and Oryza latifolia by C0t-1 DNA

WANG De-bin, WANG Yang, WU Qi, ZHAO Hou-ming, LI Gang, QIN Rui, WANG Chun-tai, LIU Hong   

  1. Engineering Research Centre for the Protection and Utilization of Bioresource in Ethnic Area of Southern China, South-Central University for Nationalities, Wuhan 430074, China
  • Received:2009-12-04 Revised:1900-01-01 Online:2010-09-28 Published:2010-09-28
  • Contact: LIU Hong
  • Supported by:

    the Science Foundation of South-Central University for Nationalities, China (Grant No. YZY10007).

Abstract: In order to reveal the origin and evolutionary relationship between two CCDD genome species, Oryza alta and Oryza latifolia, fluorescence in situ hybridization (FISH) was adopted to analyze the genomes of the two species with C0t-1 DNA from O. alta as a probe. Karyotype was also comparatively analyzed between O. alta and O. latifolia based on their similar band patterns of the hybridization signals. There were a high homology and close relationship between O. alta and O. latifolia, however, the distinction between the hybridization signals was also clear. C0t-1 DNA was proved to be species- and genome type-specific. It is suggested that C0t-1 DNA-FISH could be more efficient to analyze the genomic relationship between different species. According to the comparative analysis of highly and moderately repetitive DNA sequences between the two allotetraploidy species, O. alta and O. latifolia, the possible origin and evolutionary mechanism of allotetraploidy of Oryza were discussed.

Key words: Oryza alta, Oryza latifolia, fluorescence in situ hybridization, chromosomal repeat sequence, karyotype, phylogenetic relation