OsAMT1.1 Expression by Nitrate-Inducible Promoter of OsNAR2.1 Increases Nitrogen Use Efficiency and Rice Yield
Received date: 2022-10-17
Accepted date: 2023-01-14
Online published: 2023-01-17
Nitrate (NO3-) and ammonium (NH4+) are two main inorganic nitrogen (N) sources during crop growth. Here, we enhanced the expression of OsAMT1.1, which encodes a NH4+ transporter, using the NO3--inducible promoter of OsNAR2.1 and an ubiquitin promoter in transgenic rice plants. Under field condition of 120 kg /hm2 N, agronomic N use efficiency, N recovery efficiency and N transport efficiency, and grain yield of the pOsNAR2.1:OsAMT1.1 transgenic lines were increased compared with those of the wild type (WT) and the pUbi:OsAMT1.1 transgenic plants. Under 2.0 mmol/L NO3- + 0.5 mmol/L NH4+ and 0.5 mmol/L NO3- + 2.0 mmol/L NH4+ conditions of hydroponic culture, compared with the WT, both biomass and total N content were increased in the pOsNAR2.1:OsAMT1.1 transgenic lines. However, biomass was significantly reduced in pUbi:OsAMT1.1 transgenic plants under 0.5 mmol/L NO3- + 2.0 mmol/L NH4+ condition. The lines expressing pOsNAR2.1:OsAMT1.1 exhibited increased OsAMT1.1 expression and 15NH4+ influx in roots under both 2.0 mmol/L NO3- + 0.5 mmol/L NH4+ and 0.5 mmol/L NO3- + 2.0 mmol/L NH4+ conditions. Our study showed that expression of OsAMT1.1 can be promoted when driven by the OsNAR2.1 promoter, especially under high-level nitrate condition, leading to enhancement of NH4+ uptake, N use efficiency and grain yield.
Key words： OsAMT1.1; OsNAR2.1 promoter; nitrogen use efficiency; yield; rice
Jiang Hongzhen, Wang Yamei, Lai Liuru, Liu Xintong, Miao Changjian, Liu Ruifang, Li Xiaoyun, Tan Jinfang, Gao Zhenyu, Chen Jingguang . OsAMT1.1 Expression by Nitrate-Inducible Promoter of OsNAR2.1 Increases Nitrogen Use Efficiency and Rice Yield[J]. Rice Science, 2023 , 30(3) : 222 -234 . DOI: 10.1016/j.rsci.2023.03.006
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