
Development of Hybrid Rice Variety FY7206 with Blast Resistance Gene Pid3 and Cold Tolerance Gene Ctb1
Received date: 2016-01-04
Accepted date: 2016-02-22
Online published: 2016-06-12
Hybrid rice Fanyou 7206 (FY7206), derived from the cross between a sterile line Fanyuan A and a restorer line Fuhui 7206, was bred by the Rice Research Institute, Fujian Academy of Agricultural Sciences, China. FY7206 was characterized by moderate blast resistance, cold tolerance, as well as wide adaptability, and high yields. The blast resistance results indicated that the frequencies of blast races in race B, race C and the total resistance frequency for FY7206 were 95.5%, 100.0% and 97.2%, respectively. The disease resistance results showed that the leaf blast grade for FY7206 was level 1 and panicle blast was level 5. The indoor spray results indicated that FY7206 was resistant to 11 isolates of Magnorpathe oryzae. The blast resistance of FY7206 might be derived from the high expression of blast resistance gene Pid3. The results for simulated cold resistance in an artificial climate chamber indicated that the cold tolerance for FY7206 was moderate at the booting and flowering stages. The cold tolerance results also indicated that FY7206 could be tolerant to temperatures as low as 10 °C at the seedling stage. The qRT-PCR results showed that the expression of cold tolerance gene Ctb1 in FY7206 was relatively high. These results suggested that FY7206 is a hybrid indica rice variety with good comprehensive characteristics, including blast resistance and cold tolerance.
Key words: hybrid rice; blast resistance gene Pid3; cold tolerance gene Ctb1; yield; breeding
Hong-guang Xie, Jia-huang Jiang, Yan-mei Zheng, Yong-sheng Zhu, Fang-xi Wu, Xi Luo, Qiu-hua Cai, Jian-fu Zhang, Hua-an Xie . Development of Hybrid Rice Variety FY7206 with Blast Resistance Gene Pid3 and Cold Tolerance Gene Ctb1[J]. Rice Science, 2016 , 23(5) : 266 -273 . DOI: 10.1016/j.rsci.2016.08.004
| 1 | Chen L Y, Xiao Y H, Tang W B, Lei D Y.2007. Prospect and practice of three-procedure breeding on super hybrid rice.Chin J Rice Sci, 21(1): 90-94. (in Chinese with English abstract) |
| 2 | Chen S D, Hu B H.2003. Fifty Years of Plant Protection in China. Beijing: Chinese Agricultural Press: 24-25. (in Chinese) |
| 3 | Chen W F, Xu Z J, Zhang B L, Zhang W Z, Yang S R.2002. Prospect and progress on super high yield of rice.Chin Engin Sci, 4(1): 31-35. (in Chinese with English abstract) |
| 4 | Chen X W, Shang J J, Chen D X, Lei C L, Zou Y, Zhai W X, Liu G Z, Xu J C, Ling Z Z, Cao G, Ma B T, Wang Y P, Zhao X F, Li S G, Zhu L H.2006. A B-lectin receptor kinase gene conferring rice blast resistance.Plant J, 46: 794-804. |
| 5 | Cheng S H.2010. Super Rice Breeding of China. Beijing: Scientific Press. |
| 6 | Han L Z, Sheng J S, Cao G L.2001. Research on cold tolerance in rice of Korea.World Agric, 12: 36-37. (in Chinese with English abstract) |
| 7 | Han L Z, Koh H J, Piao Z Z.2002. Status and prospects of genetic and QTLs analysis for cold tolerance in rice.Chin J Rice Sci, 16(2): 193-198. (in Chinese with English abstract) |
| 8 | Khan D R, Mackill D J, Vergara B S.1986. Selection for tolerance to low temperature-induced spikelet sterility at anthesis in rice.Crop Sci, 26(4): 694-698. |
| 9 | Lei J C, You N S, Huang L X, Lei S P, Zheng X H, Zhu X T.1998. Breeding and utilization of indica sterile line Fuyi A. Hybrid Rice, 3: 8-11. (in Chinese with English abstract) |
| 10 | Li C G, Wang B L, Song X H, Liu Y W, Meng Q X, Song D M, Cheng F Y.2012. Research progress of QTLs analysis for cold tolerance in rice in China.Heilongjiang Agric Sci, (4): 138-141. (in Chinese with English abstract) |
| 11 | Li M R, Liu H X, Wang Y R.2000. Advance in researches on molecular biology of plant cold tolerance.J Tropic Subtropic Bot, 8(1): 70-80. (in Chinese with English abstract) |
| 12 | Li X, Zhang L J.2015. Advance on research of rice blast control with biological diversity.South Agric, 15: 34-35. (in Chinese with English abstract) |
| 13 | Li Y Z.2007. Advance in plant hormone study.J Henan Inst Sci Technol, 35(4): 50-53. (in Chinese with English abstract) |
| 14 | Liu Z L, Lei C L, Cheng Z J, Li W, Wang J L, Shi K.2007. Advance on mapping and cloning of blast resistance in rice.Crops, (3): 16-19. (in Chinese with English abstract) |
| 15 | Lu M H, Liu W C, Zhu F, Zhang Q F, Xia F.2015. Analysis on the reasons and countermeasures of rice blast.China Plant Protect, 35(6): 35-39. (in Chinese with English abstract) |
| 16 | Lu T T.2012. Primarily QTLs Mapping of Cold Tolerance in Rice. Changsha, China: Central South University. (in Chinese) |
| 17 | Ma Y, Dai X Y, Xu Y Y, Luo W, Zheng X M, Zeng D L, Pan Y J, Lin X L, Liu H H, Zhang D J, Xiao J, Guo X Y, Xu S J, Niu Y D, Jin J B, Zhang H, Xu X, Li L G, Wang W, Qian Q, Ge S, Chong K.2015. COLD1 confers chilling tolerance in rice.Cell, 160: 1-13. |
| 18 | Saito K, Hayano-Saito Y, Kuroki M, Sato Y.2010. Map-based cloning of the rice cold tolerance gene Ctb1.Plant Sci, 179: 97-102. |
| 19 | Shang J J, Tao Y, Chen X W, Yan Z, Lei C L, Wang J, Li X B, Zhao X F, Zhang M J, Lu Z K, Xu J C, Cheng Z K, Wan J M, Zhu L H.2009. Identification of a new rice blast resistance gene, Pid3, by genome wide comparison of paired nucleotide-binding site: Leucine-rich repeat genes and their pseudo-gene alleles between the two sequenced rice genomes.Genetics, 182: 1303-1311. |
| 20 | Song D M, He M, Meng Z H, Meng Q X, Li C G, Cheng F Y.2011. Analysis on research status and breeding strategy of rice cold damage.North Rice, 41(2): 71-72, 78. (in Chinese with English abstract) |
| 21 | Song G S, Sun Z F, Sun L, Du K M, Wang X.2011. Comparison between physiological properties and cold tolerance under low temperature treatment during different growing stages of rice in northeast central region of China.Acta Ecol Sin, 31(13): 3788-3795. |
| 22 | Sthapit B R, Witcombe J R.1998. Inheritance of tolerance to chilling stress in rice during germination and plumule greening.Crop Sci, 38(3): 660-665. |
| 23 | Wang H, Zhou G X, Chen J J, Yan Z, Fang Y, Huang Y L, Zhang C H, Zhang Y H, Chen L.2014. Research progress and prospect in rice blast resistance genes.Hybrid Rice, 29(6): 1-5. (in Chinese with English abstract) |
| 24 | Wang Y R.1995. Rice Physiological Breeding. Beijing: Reference Press of Science and Technology: 419-420. (in Chinese) |
| 25 | You N S, Zhang Y H, Huang L X, Lei S P, Pan Y Q, Hu R H, Wu Z Y, Xie M Z, Meng Z G.2014. Breeding of the CMS line Fanyuan A with good aroma quality and fine blast resistance in rice.Chin J Tropic Crops, 35(3): 488-492. (in Chinese with English abstract) |
| 26 | Zhao Z W, Li S G, Huang W Z, Lei S F.2006. Development and foreground of cold tolerance in different low temperature sensitive stage of rice.Southwest China J Agric Sci, 19(2): 330-335. (in Chinese with English abstract) |
| 27 | Zheng J T, You N S, Huang T X, Zhao M F, Zhang S J, Xie H A.2010. Advance on breeding of hybrid rice for high quality, high yield, multiple resistance in Fujian Province, China.J Sci Technol Rice Wheat Fujian, China, 28(3): 1-8. (in Chinese with English abstract) |
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