Development of Hybrid Rice Variety FY7206 with Blast Resistance Gene Pid3 and Cold Tolerance Gene Ctb1

doi:10.1016/j.rsci.2016.08.004

Abstract

Abstract:

Hybrid rice Fanyou 7206 (FY7206), derived from the cross between a sterile line Fanyuan A and a restorer line Fuhui 7206, was bred by the Rice Research Institute, Fujian Academy of Agricultural Sciences, China. FY7206 was characterized by moderate blast resistance, cold tolerance, as well as wide adaptability, and high yields. The blast resistance results indicated that the frequencies of blast races in race B, race C and the total resistance frequency for FY7206 were 95.5%, 100.0% and 97.2%, respectively. The disease resistance results showed that the leaf blast grade for FY7206 was level 1 and panicle blast was level 5. The indoor spray results indicated that FY7206 was resistant to 11 isolates of Magnorpathe oryzae. The blast resistance of FY7206 might be derived from the high expression of blast resistance gene Pid3. The results for simulated cold resistance in an artificial climate chamber indicated that the cold tolerance for FY7206 was moderate at the booting and flowering stages. The cold tolerance results also indicated that FY7206 could be tolerant to temperatures as low as 10 °C at the seedling stage. The qRT-PCR results showed that the expression of cold tolerance gene Ctb1 in FY7206 was relatively high. These results suggested that FY7206 is a hybrid indica rice variety with good comprehensive characteristics, including blast resistance and cold tolerance.

Key words: hybrid rice, blast resistance gene Pid3, cold tolerance gene Ctb1, yield, breeding

Hong-guang Xie, Jia-huang Jiang, Yan-mei Zheng, Yong-sheng Zhu, Fang-xi Wu, Xi Luo, Qiu-hua Cai, Jian-fu Zhang, Hua-an Xie. Development of Hybrid Rice Variety FY7206 with Blast Resistance Gene Pid3 and Cold Tolerance Gene Ctb1[J]. Rice Science, 2016, 23(5): 266-273.

Figures/Tables 12

References 27

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Gene	Forward primer	Reverse primer
β-actin	TGTAAGCAACTGGGATGA	CCTTCGTAGATTGGGACT
Ctb1	AATAACACCTGGAACATA	ATAACAAGCAATCTATCAC
Pid3	GGTGCGAAGTTGCCATTG	TTCTGAAGTAGAGGAGGAG GTT

Gene	Forward primer	Reverse primer
β-actin	TGTAAGCAACTGGGATGA	CCTTCGTAGATTGGGACT
Ctb1	AATAACACCTGGAACATA	ATAACAAGCAATCTATCAC
Pid3	GGTGCGAAGTTGCCATTG	TTCTGAAGTAGAGGAGGAG GTT

Variety	Race-resistance for Magnaporthe oryzae			Leaf blast grade	Panicle blast grade
Variety	Race B (%)	Race C (%)	Ratio of race-resistance (%)	Leaf blast grade	Panicle blast grade
Fanyou 7206	95.5	100.0	97.2	1	5
Jinyougui 99 (CK)	77.3	76.9	77.8	4	7

Variety	Race-resistance for Magnaporthe oryzae			Leaf blast grade	Panicle blast grade
Variety	Race B (%)	Race C (%)	Ratio of race-resistance (%)	Leaf blast grade	Panicle blast grade
Fanyou 7206	95.5	100.0	97.2	1	5
Jinyougui 99 (CK)	77.3	76.9	77.8	4	7

Variety	No. of plants before 10 °C cold treatment	No. of survival plants after 10 °C cold treatment	Plant survival ratio (%)
Fanyuan A	50	45.3 ± 2.5 b	90.7 ± 5.0 b
Fanyou 7206	50	42.3 ± 2.3 bc	84.7 ± 4.6 bc
Fuhui 7206	50	4.0 ± 1.7 d	8.0 ± 3.5 d
Zhonghua 11	50	49.7 ± 0.6 a	99.3 ± 1.2 a