CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome
Received date: 2018-06-09
Accepted date: 2018-07-26
Online published: 2018-12-18
SUMMARY Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor (ABE)-nCas9 tool that induced targeted A?T to G?C point mutation of a key single nucleotide polymorphism site in an important agricultural gene. Combined with the modified single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in the rice genome.
Hao Li, Ruiying Qin, Xiaoshuang Liu, Shengxiang Liao, Rongfang Xu, Jianbo Yang, Pengcheng Wei . CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome[J]. Rice Science, 2019 , 26(2) : 125 -128 . DOI: 10.1016/j.rsci.2018.07.002
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