Editing of Rice Isoamylase Gene ISA1 Provides Insights into Its Function in Starch Formation
Received date: 2018-06-01
Accepted date: 2018-07-27
Online published: 2018-12-18
Isoamylase 1 (ISA1) is an isoamylase-type debranching enzyme which plays a predominant role in amylopectin synthesis. In this study, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease 9 (CRISPR/Cas9) system was used to edit ISA1 gene in rice via Agrobacterium-mediated transformation. We identified 36 genetic edited lines from 55 T0 transgenic events, and classified the mutation forms into 7 types. Of those, two homozygous mutants, cr-isa1-1 (type 1, with an adenine insertion) and cr-isa1-2 (type 3, with a cytosine deletion) were selected for further analysis. Seed sizes of both cr-isa1-1 and cr-isa1-2 were affected, and the two mutants also displayed a shrunken endosperm with significantly lower grain weight. Electron microscopy analysis showed that abnormal starch granules and amyloplasts were found in cr-isa1-1 and cr-isa1-2 endosperm cells. The contents of total starch, amylose and amylopectin in the endosperm of the cr-isa1 mutants were significantly reduced, whereas sugar content and starch gel consistency were observably increased compared to the wild-type. The gelatinization temperature and starch chain length distributions of the cr-isa1 mutants were also altered. Moreover, transcript levels of most starch synthesis-related genes were significantly lower in cr-isa1 mutants. In conclusion, the results indicated that gene edition of ISA1 affected starch synthesis and endosperm development, and brought potential implications for rice quality breeding.
Key words： ISA1; rice; starch biosynthesis; starch granule; physicochemical property; CRISPR; Cas9
Shufen Chao, Yicong Cai, Baobing Feng, Guiai Jiao, Zhonghua Sheng, Ju Luo, Shaoqing Tang, Jianlong Wang, Peisong Hu, Xiangjin Wei . Editing of Rice Isoamylase Gene ISA1 Provides Insights into Its Function in Starch Formation[J]. Rice Science, 2019 , 26(2) : 77 -87 . DOI: 10.1016/j.rsci.2018.07.001
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