Screening for Spikelet Fertility and Validation of Heat Tolerance in a Large Rice Mutant Population
Received date: 2018-03-31
Accepted date: 2018-08-22
Online published: 2019-04-04
A total of 10 000 M4 individuals in Jao Hom Nil (JHN) mutant population was treated with high temperature (40 ºC to 45 ºC) during the day time (6 h) from the booting to the harvesting stages, and ambient temperature (33 ºC to 35 ºC) was used as the control. The results of screening and yield trials found that the mutant line M9962 had a high spikelet fertility of 78% under heat stress. In addition, the other mutant lines, including M3181 and M7988, had a spikelet fertility of approximately 70%. However, the JHN wild type, Sin Lek, RD15 and RD33 had very low spikelet fertility of 34%, 14%, 9% and 4%, respectively. The lower spikelet fertility at an elevated temperature resulted in a dramatic decrease of filled grain and contributed to a loss in 100-grain weight. M9962 is a potential genetic stock for use in a heat tolerance breeding programme. In addition, spikelet fertility at high temperature was representative of heat tolerance and can be used as a screening trait for heat tolerance during the reproductive phase on a large scale.
Key words： high temperature; rice; spikelet fertility; heat tolerance; mutant
Cheabu Sulaiman, Panichawong Nat, Rattanametta Prisana, Wasuri Boonthong, Kasemsap Poonpipope, Arikit Siwaret, Vanavichit Apichart, Malumpong Chanate . Screening for Spikelet Fertility and Validation of Heat Tolerance in a Large Rice Mutant Population[J]. Rice Science, 2019 , 26(4) : 229 -238 . DOI: 10.1016/j.rsci.2018.08.008
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