Morphological and Molecular Characterization of Novel Salt-Tolerant Rice Germplasms from the Philippines and Bangladesh
Received date: 2018-05-08
Accepted date: 2018-09-28
Online published: 2019-01-25
To screen for new sources of salinity tolerance, 688 traditional rice varieties from the Philippines and Bangladesh were obtained, and their tolerance to hypersaline conditions at the seedling stage was examined. A total of 29 Philippine lines and 15 Bangladeshi lines were scored as salt-tolerant. Morphological assessment (plant height, biomass and Na-K ratio) revealed that among the 44 salt-tolerant accessions, Casibon, Kalagnon and Ikogan had significantly higher relative shoot length difference, relative shoot growth reduction and shoot Na-K ratio than the tolerant check FL478. Additionally, AC and Akundo exhibited significantly higher Na-K ratios than the other genotypes. The genetic diversity of the 44 genotypes was assessed using 34 simple sequence repeat markers. A total of 133 alleles were detected across all loci. Cluster analysis showed that AC, Akundo and Kuplod were clustered along with FL478, indicating a strong genetic relatedness between these genotypes. IR29 (susceptible check) was singly separated. The haplotype analysis revealed that none of the 44 genotypes had a similar allele combination as FL478. These accessions are of interest since each genotype might be different from the classical salinity-tolerant Pokkali.
Key words： rice; simple sequence repeat; Na-K ratio; salinity tolerance; traditional landrace
F. Aala Jr Wilson, B. Gregorio Glenn . Morphological and Molecular Characterization of Novel Salt-Tolerant Rice Germplasms from the Philippines and Bangladesh[J]. Rice Science, 2019 , 26(3) : 178 -188 . DOI: 10.1016/j.rsci.2018.09.001
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