Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System
Received date: 2018-10-30
Accepted date: 2018-12-18
Online published: 2018-12-18
SUMMARY Rice is an important food crop in China and the development of hybrid rice is a crucial way to increase grain yield. The creation of dual-purpose nuclear-sterile lines for two-line hybrid breeding has become vital for commercial rice breeding. We constructed the pC1300-2x35S::Cas9-sgRNAPTGMS2-1 expression vector for editing the male fertility gene PTGMS2-1 in two widely compatible rice varieties 93-11 and Huazhan by using the CRISPR/Cas9 system. We obtained the marker-free photoperiod-/thermo- sensitive genic male-sterile (P/TGMS) lines in T1 generation. According to the experiments in phytotron with four temperature and photoperiod treatments we found the temperature is the main factor for restoring the pollen fertility of ptgms2-1 mutants in 93-11 and Huazhan and the photoperiod also has some effects on pollen fertility in two different rice backgrounds. The application of cultivating new male-sterile lines by genome editing system will significantly accelerate the rice breeding process.
Lan Shen, Guojun Dong, Yu Zhang, Guocheng Hu, Qiang Zhang, Guanglian Hu, Bo Xu, Deyong Ren, Jiang Hu, Li Zhu, Zhenyu Gao, Guangheng Zhang, Longbiao Guo, Dali Zeng, Qian Qian . Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System[J]. Rice Science, 2019 , 26(2) : 129 -132 . DOI: 10.1016/j.rsci.2018.12.006
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