A Fragment Substitution in Promoter of <i>MS92/PTC1</i> Causes Male Sterility in Rice

Peng Qin, Luchang Deng, Weilan Chen, Juan Huang, Shijun Fan, Bin Tu, Jun Tan, Hua Yuan, Yuping Wang, Bingtian Ma, Shigui Li

doi:10.1016/j.rsci.2020.03.004

Rice Science >

2020 , Vol. 27 >Issue 5: 396 - 404

DOI: https://doi.org/10.1016/j.rsci.2020.03.004

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A Fragment Substitution in Promoter of MS92/PTC1 Causes Male Sterility in Rice

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¹Rice Research Institute / State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
²Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
³Characteristic Crop Research Institute, Chongqing Academy of Agricultural Sciences, Chongqing 402160, China; #These authors contributed equally to this work

Corresponding authors: Li Shigui (lishigui@sicau.edu.cn)

Received date: 2019-11-06

Accepted date: 2020-03-25

Online published: 2020-09-28

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Abstract

Persistent tapetal cell1 (PTC1) plays a curial role in pollen development, and is thought to function as a transcriptional activator in rice. However, the molecular mechanism of PTC1 in regulating pollen development and its cis-elements are not well understood. We identified a novel weak male sterility mutant (ms92) which exhibited expanded tapetum and shrink pollen grains. Map-based cloning and allelic analysis suggested that the male sterility of ms92 was caused by a DNA fragment substitution in the promoter of PTC1. The decreased expression of MS92/PTC1 in ms92 and cis-element analysis indicated that the substituted sequence contained several potential binding cis-element of negative feedback. MS92/PTC1 was specifically expressed in tapetum and microspores at the young microspore stage, and its protein was localized in nucleus. We further found that MS92/PTC1 functions as a transcription activator by recognizing H3K4me3. Transcriptomic analysis revealed that a number of genes involved in tapetum degeneration and pollen wall formation were down-regulated in ms92, which are the potential targets of MS92/PTC1. The substitution fragment in MS92/PTC1 promoter was essential for pollen development, and we provided a novel mutant for further identifying the cis-elements in promoter and the molecular network of MS92/PTC1.

Key words： rice; MS92/PTC1; plant homeodomain finger; cis-element; male sterility; anther

Cite this article

Peng Qin, Luchang Deng, Weilan Chen, Juan Huang, Shijun Fan, Bin Tu, Jun Tan, Hua Yuan, Yuping Wang, Bingtian Ma, Shigui Li . A Fragment Substitution in Promoter of MS92/PTC1 Causes Male Sterility in Rice[J]. Rice Science, 2020 , 27(5) : 396 -404 . DOI: 10.1016/j.rsci.2020.03.004

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