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A Method for Effectively Overcoming Tight Functional Linkage Between Genes in Rice by CRISPR/Cas9 System

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  • 1State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, China
    2Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
    3College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China

# These authors contributed equally to this work

Received date: 2019-07-11

  Accepted date: 2019-10-01

  Online published: 2020-01-17

Cite this article

Sanfeng Li, Lan Shen, Ping Hu, Xianmei Wu, Qiaoling Yuan, Yuchun Rao, Qian Qian, Kejian Wang, Xudong Zhu, Lianguang Shang, Yuexing Wang . A Method for Effectively Overcoming Tight Functional Linkage Between Genes in Rice by CRISPR/Cas9 System[J]. Rice Science, 2020 , 27(3) : 180 -183 . DOI: 10.1016/j.rsci.2020.04.002

References

[1] Aizza L C B, Dornelas M C. 2011. A genomic approach to study anthocyanin synthesis and flower pigmentation in passionflowers.J Nucl Acids, 2011: 371517.
[2] Baltes N J, Voytas D F.2015. Enabling plant synthetic biology through genome engineering.Trends Biotechnol, 33(2): 120-131.
[3] Fan F J, Fan Y Y, Du J H, Zhuang J Y.2007. Fine mapping of C(chromogen for anthocyanin) gene in rice. Chin J Rice Sci, 21(5): 454-458. (in Chinese with English abstract)
[4] Fukuoka S, Saka N, Koga H, Ono K, Shimizu T, Ebana K, Hayashi N, Takahashi A, Hirochika H, Okuno K, Yano M.2009. Loss of function of a proline-containing protein confers durable disease resistance in rice.Science, 325: 998-1001.
[5] Hou F Y, Wang Q M, Li A X.2009. Study progress on anthocyanidin synthase of plants.Chin Agric Sci Bull, 25(21): 188-190.
[6] Huang S W, Weigel D, Beachy R N, Li J Y.2016. A proposed regulatory framework for genome-edited crops.Nat Genet, 48(2): 109-111.
[7] Li M R, Li X X, Zhou Z J, Wu P Z, Fang M C, Pan X P, Lin Q P, Luo W B, Wu G J, Li H Q.2016. Reassessment of the four yield-related genesGn1a, DEP1, GS3, and IPA1 in rice using a CRISPR/Cas9 system. Front Plant Sci, 7: 377.
[8] Li Y B.2011. Cloning and functional analysis of a major QTL, GS5 for grain size/weight and Chalk5 for chalkiness rate in rice. [PhD Thesis]. Wuhan, China: Huazhong Agricultural University.
[9] Lin T Z, Sun L T, Gong H B, Wang Y H, Liu L L, Zhao Z G, Jiang L, Wan J M.2019. Identification and gene mapping of awhite-stripe leaf after transplanting at low temperature mutant in rice. Chin J Rice Sci, 33(1): 1-11.
[10] Luo X, Ji S D, Yuan P R, Lee H S, Kim D M, Balkunde S, Kang J W, Ahn S.2013. QTL mapping reveals a tight linkage between QTLs for grain weight and panicle spikelet number in rice.Rice, 6(1): 33.
[11] Pang A Y, Jin L, Chen J Y, Liu C L, Ruan Y.2018. Obtain of double mutant of closely linked genesM320 and M330 by CRISPR/Cas9 system. Mol Plant Breeding, 16(13): 4301-4307.
[12] Reddy A R.1996. Genetic and molecular analysis of the anthocyanin pigmentation pathway in rice.Plant Mol Biol, 32(4): 735-743.
[13] Saitoh K, Onishi K, Mikami I, Thidar K, Sano Y.2004. Allelic diversification at theC (OsC1) locus of wild and cultivated rice: Nucleotide changes associated with phenotypes. Genetics, 168(2): 997-1007.
[14] Sakamoto W, Ohmori T, Kageyama K, Miyazaki C, Saito A, Murata M, Noda K, Maekawa M.2001. ThePurple leaf (Pl) locus of rice: The Pl(w) allele has a complex organization and includes two genes encoding basic helix-loop-helix proteins involved in anthocyanin biosynthesis. Plant Cell Physiol, 42: 982-991.
[15] Shao G N, Xie L H, Jiao G A, Wei X J, Sheng Z H, Tang S Q, Hu P S.2017. CRISPR/CAS9-mediated editing of the fragrant geneBadh2 in rice. Chin J Rice Sci, 31(2): 216-222. (in Chinese with English abstract)
[16] Sheng S L, Yu B, Li C, Lu J F.2008. Study on the location of chromogen gene and its physical distance from broad-affinity genes.Acta Agric Jiangxi, 20(9): 27-28. (in Chinese with English abstract)
[17] Sun Y W, Jiao G A, Liu Z P, Zhang X, Li J Y, Guo X P, Du W M, Du J L, Francis F, Zhao Y D, Xia L Q.2017. Generation of high- amylose rice through CRISPR/Cas9-mediated targeted mutagenesis of starch branching enzymes.Front Plant Sci, 8: 298.
[18] Tang L, Mao B G, Li Y K, Lv Q M, Zhang L P, Chen C Y, He H J, Wang W P, Zeng X F, Shao Y, Pan Y L, Hu Y Y, Peng Y, Fu X Q, Li H Q, Xia S T, Zhao B R.2017. Knockout ofOsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulating indica rice without compromising yield. Sci Rep, 7: 14438.
[19] Zhang J S, Zhang H, Botella J R, Zhu J K.2018. Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of theWaxy gene in elite rice varieties. J Integr Plant Biol, 60(5): 369-375.
[20] Zhao S S, Wang C H, Ma J, Wang S, Tian P, Wang J L, Cheng Z J, Zhang X, Guo X P, Lei C L.2016. Map-based cloning and functional analysis of the chromogen geneC in rice(Oryza sativa L.). J Plant Biol, 59(5): 496-505.
[21] Zhou H, He M, Li J, Chen L, Huang Z F, Zheng S Y, Zhu L Y, Ni E D, Jiang D G, Zhao B R, Zhuang C X.2016. Development of commercial thermo-sensitive genic male sterile rice accelerates hybrid rice breeding using the CRISPR/Cas9-mediatedTMS5 editing system. Sci Rep, 6: 37395.
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