Molecular-Cytological Identification and Chromosome Behavior Analysis of Telotetrasomic in Rice

摘要/Abstract

摘要： From the progenies of a telotrisomic of chromosome 9 short arm of an indica rice variety, Zhongxian 3037, a phenotypical variant was selected. The variant plant had rolled leaves, dispersed plant type, as well as a low seed-setting rate. Cytological and molecular cytological investigations revealed two extra chromosomes, which were the shortest in somatic cells of the variant. Fluorescent in situ hybridization (FISH) analysis using a rice centromere specific DNA (RCS2) and a DNA sequence specific for chromosome 9 on premetaphase and pachytene chromosomes showed that these two chromosomes were the short arms of chromosome 9. That is to say, the variant was a telotetrasomic of chromosome 9. Among the 25 pachytene cells, the two telosomic chromosomes paired each other to form a bivalent and didn’t pair with other normal chromosome 9 as multivalents in 96% cells. However, the bivalent was easy to disassociate in advance.

关键词: rice, telotetrasomic, meiosis, fluorescent in situ hybridization, molecular cytology, chromosome beh

GONG Zhi-yun, GAO Qing-song, YU Heng-xiu, YI Chuan-deng, GU Ming-hong. Molecular-Cytological Identification and Chromosome Behavior Analysis of Telotetrasomic in Rice[J]. RICE SCIENCE, 2008, 15(3): 161-165 .

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