RICE SCIENCE ›› 2009, Vol. 16 ›› Issue (2): 124-130 .DOI: 10.1016/S1672-6308(08)60068-2

• 研究报告 • 上一篇    下一篇

Observation on Double Fertilization and Early Embryonic Development in Autotetraploid Polyembryonic Rice

DAI Xi-mei; YANG Xu; HUANG Qun-ce; QIN Guang-yong   

  1. Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou 450052, China
  • 收稿日期:2008-12-26 出版日期:2009-06-28 发布日期:2009-06-28
  • 通讯作者: DAI Xi-mei
  • 基金资助:
    the National Science & Technology Pillar Program of China in the Tenth Five-Year Plan Period (Grant No. 2001BA302B) and the Education Department of Henan Province, China (Grant No. 2008A208019).

摘要: The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnormalities including degenerated ovary, abortive embryo sac, single fertilization, double-ovule and double-embryo and so on. were found during double fertilization and embryo development in IR36-Shuang. The rate of the abnormalities was 46.67% in IR36-Shuang, significantly higher than that in the control, an autotetraploid rice line IR36-4X (33.00%). Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang.

关键词: rice, autotetraploid polyembryony, double fertilization, reproductive developmental character, developmental biology, embryonic development