Mapping a Rice Glabrous Gene Using Simple Sequence Repeat Markers

doi:10.1016/S1672-6308(08)60063-3

摘要/Abstract

摘要： Inheritance analysis and gene mapping of the glabrous trait were conducted using the crosses between a pubescent rice material Chuanxiang 29B (an aromatic elite maintainer line of hybrid rice), and two glabrous rice materials, Lemont and R2 (R2 is a new restorer line of hybrid rice). All F1 plants from the two crosses had pubescent leaves, confirming that the pubescence trait in Chuanxiang 29B is dominant over the glabrous trait. In F₂ individuals, the segregation ratio of three pubescent to one glabrous plant suggests that a single glabrous gene was present in Lemont and R2. Using the bulked segregant analysis, the linkage analysis of the simple sequence repeat markers showed that the glabrous gene, gl-1, was flanked by GL311 and GL8 with the genetic distances of 0.19 and 0.92 cM on chromosome 5, respectively.

关键词: rice, Oryza sativa, glabrous leaf, genetic analysis, gene mapping, simple sequence repeat, glabrous gene

WANG Dong, SUN Shu-xia, GAO Fang-yuan, LU Xian-jun, LI Zhi-hua, REN Guang-jun. Mapping a Rice Glabrous Gene Using Simple Sequence Repeat Markers[J]. RICE SCIENCE, 2009, 16(2): 93-98 .

参考文献

1 Foster K W, Rutger J N. Independent segregation of semi- dwarfing genes and a gene for pubescence in rice. Heredity, 1978, 69: 137–138.
2 Moose S P, Lauter N, Carlson S R. The maize macrohairless1 locus specifically promotes leaf blade macrohair initiation and responds to factors regulating leaf identity. Genetics, 2004, 166: 1451–1461.
3 Leisle D. Genetics of leaf pubescence in wheat. Crop Sci, 1974, 14: 173–174.
4 McIntosh R A, Yamazaki Y, Devos K M, Dubcovsky J, Rogers W J, Appels R. Catalogue of gene symbols for wheat. http://www.grs.nig.ac.jp/wheat/komugi/genes/, 2003.
5 Sato K, Takeda K. Genetic analysis of large trichomes on the barley leaf blade. Barley Genet Newsl, 1992, 22: 50–52.
6 Sarkarung S, Collins F. Inheritance of leaf pubescence in oats. Agron Abstr, 1977, 77: 7.
7 Kumar K, Andrews D. Genetics of qualitative traits in pearl millet: A review. Crop Sci, 1993, 33: 1–20.
8 Gibson P, Maiti R. Trichomes in segregating generations of Sorghum matings: Inheritance of presence and density. Crop Sci, 1983, 23: 73–75.
9 Luo J, Hu P S. American glabrous rice utilized in breeding of super-high-yielding and good-quality varieties. Chinese Rice Res Newsl, 2000, 8(2): 3–5.
10 Xu X F, Mei H W, Luo L J, Cheng X N, Li Z K. RFLP- facilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvata lugens). Theor Appl Genet, 2002, 104: 248–253.
11 Dong Y J, Kamiunten H, Ogawa T, Tsuzuki E, Terao H, Lin D Z, Matsuo M. Mapping of QTLs for leaf developmental behavior in rice (Oryza sativa L.). Euphytica, 2004, 138: 169– 175.
12 Yamagata H. Differentiation and inheritance of characters. Chapter 2. Inheritance of morphological characters. In: Matsuo T, Futsuhara Y, Kikuchi F, Yamaguchi H. Science of the Rice Plant. Genetics. Vol. 3. Tokyo, Japan: Food and Agriculture Policy Research Center, 1997: 277–285.
13 Yu Z H, McCouch S R, Tanksley S D, Kinoshita T, Sato S. Association of morphological and RFLP markers in rice (Oryza sativa L.). Genome, 1995, 38(3): 566–574.
14 Dobrovolskaya O, Pshenichnikova T, Arbuzova V, Lohwasser U, Röder M, Börner A. Molecular mapping of genes determining hairy leaf character in common wheat with respect to other species of the Triticeae. Euphytica, 2007, 155: 285–293.
15 Wu P, Zhang G, Ladha J K, McCouch S R. Molecular- marker-facilitated investigation on the ability to stimulate N2 fixation in the rhizosphere by irrigated rice plants. Theor Appl Genet, 1995, 91: 1177–1183.
16 Oka H I. Phylogenetic differentiation of the cultivated rice: 1. Variations in various characters and character combinations among rice varieties. Jpn J Breeding, 1953, 3: 33–43.
17 Sato S, Muraoka K, Sano Y. Reconstruction of a linkage group corresponding to Nishimura’s second chromosome in rice. Jpn J Breeding, 1982, 32(3): 232–238.
18 Nagao S, Takahashi M. Genetical studies on rice plant: 28. Trial construction of twelve linkage groups in Japanese rice. J Fac Agric Hokkaido Univ, 1963, 53(1): 72–130.
19 Iwata N, Omura T, Ditto I V. On the possibility of association of three linkage groups with one chromosome. Jpn J Genet, 1976, 51: 135–137.
20 Iwata N, Omura T, Satoh H. Linkage studies in rice: The sequence of genes at the eighth and eleventh linkage groups. Jpn J Breeding, 1978, 28 (suppl 1): 170–171.
21 Roy B A, Stanton M L, Eppley S M. Effects of environmental stress on leaf hair density. J Evol Biol, 1999, 12: 1089–1103.
22 Pan Q H, Hu Z D, Takatoshi T, Wang L. Fine mapping of the blast resistance gene Pi15, linked to Pii, on rice chromosome 9. Acta Bot Sin, 2003, 45: 871–877. (in Chinese with English abstract)
23 Chen J W, Wang L, Pang X F, Pan Q H. Genetic analysis and fine mapping of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph19(t). Mol Genet Gen, 2006, 275: 321–329.
24 Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321– 4325.
25 Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregation analysis: A rapid method to detect markers in specific genomic regions by using segregation population. Proc Natl Acad Sci USA, 1991, 88: 9828–9832.
26 Xu S B, Tao Y F, Yang Z Q, Chu J Y. A simple and rapid method used for silver staining and gel preservation. Hereditas, 2002, 24: 335–336.
27 Li Q, Wan J M. SSRHunter: Development of a local searching software for SSR sites. Hereditas, 2005, 27(5): 808–810.
28 Lincolin S, Daly M, Lander E. Constructing genetic maps with MAPMAKER/EXP 3.0. In: Whitehead Institute Technical Report. 2nd edn. Massachusetts: Whitehead Institute Cambridge, 1992.
29 Maystrenko O I. Identification and localization of genes controlling leaf hairiness of young plants in common wheat. Genetica, 1976, 12(5): 5–15.
30 Taketa S, Chang C L, Ishii K, Takeda K. Chromosome arm location of the gene controlling leaf pubescence of a Chinese local wheat cultivar ‘Hong-mangmai’. Euphytica, 2002, 125: 141–147.
31 Pshenichnikova1 T A, Lapochkina I F, Shchukina1 L V. The inheritance of morphological and biochemical traits introgressed into common wheat (Triticum aestivum L.) from Aegilops speltoides Tausch. Genet Res Crop Evol, 2007, 54: 287–293.

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[11]	. [J]. Rice Science, 2021, 28(3): 217-232.
[12]	. [J]. Rice Science, 2019, 26(2): 118-124.
[13]	. [J]. Rice Science, 2019, 26(2): 77-87.
[14]	. [J]. Rice Science, 2019, 26(2): 88-97.
[15]	. [J]. Rice Science, 2019, 26(2): 98-108.