Development and Application of a Novel Functional Marker for Fragrance in Rice

doi:10.1016/j.rsci.2023.03.002

Abstract

Sun Pingyong, Zhang Wuhan, Zhang Li, Shu Fu, He Qiang, Xu Na, Peng Zhirong, Zeng Jia, Fang Pengpeng, Deng Huafeng. Development and Application of a Novel Functional Marker for Fragrance in Rice[J]. Rice Science, 2023, 30(3): 176-180.

Figures/Tables 1

Fig. 1. Verification and application of functional marker for OsBADH2. A, Verification of functional marker FM-E14. The green triangle is Chuan 7, the blue ones are 316S and 15 wild type homozygous plants, and the red ones are heterozygous. B, Verification of badh2-E14 genotype by sequencing in breeding population. 1, 3, 5, 7, 15 and 16 are heterozygous, and the other 15 plants are wild type homozygous. C, Development of backcross populations. Take 318S//318S/Chuan 7 as an example. MAS, Molecular assisted selection. D, Identification of OsBADH2 genotype of breeding population by functional marker FM-E14. The gray circle is a blank control. The green triangle is Chuan 7, the blue triangles are 318S and wild type homozygous plants, and the red ones are heterozygous. E, Polymorphic screening by linkage marker L-E14. M, Marker; 1, Chuan 7; 2-8 are sterile lines 318S, 316S, S373, 628S, Shuang 1S, H175S and Y58S, respectively; 9-20 are maintain lines or conventional varieties Taifeng B, Rongkang B, Nongxiang 42, Jingeng 818, Yuehesimiao, Ninggeng 8, Ning 84, Changlizaodao, Momi, Huazhan, Laowozaodao 2 and Wuyungeng 35, respectively. F, Identification of OsBADH2 genotype in the 318S//318S/Chuan 7 population. M, Marker. 1 and 2 are Chuan 7 and 318S, respectively, and 3 to 26 are part of individual plants.

References 36

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