Hydrogen Sulfide Improves Rice Seed Germination by Regulating Aluminum Absorption, Internal Antioxidant Enzyme System and Osmotic Balance under Aluminum Toxicity Conditions

doi:10.1016/j.rsci.2023.05.001

Abstract

Wei Qianqian, Kong Yali, Xiang Xingjia, Zhu Lianfeng, Liu Jia, Tian Wenhao, Jin Qianyu, Yu Yijun, Zhang Junhua, Zhu Chunquan. Hydrogen Sulfide Improves Rice Seed Germination by Regulating Aluminum Absorption, Internal Antioxidant Enzyme System and Osmotic Balance under Aluminum Toxicity Conditions[J]. Rice Science, 2023, 30(4): 271-275.

Figures/Tables 1

Fig. 1. Characteristics in germinating rice seeds under different aluminum (Al) and NaHS treatments. A, Al content. B, Hydrogen sulfide (H2S) content. C, H2O2 content. D, O2·- content. E, Malonaldehyde (MDA) content. F, Superoxide dismutase (SOD) activity. G, Peroxidase (POD) activity. H, Catalase (CAT) activity. I, Ascorbate peroxidase (APX) activity. J, Ascorbic acid (AsA) content. K, Glutathione (GSH) content. L, Al content in cell wall. M, Uronic acid in pectin content. N, Pectin methyl esteras (PME) activity. O, Relative expression of OsSTAR1. P, Relative expression of OsSTAR2. Q, Relative expression of OsNRAT1. R, Relative expression of OsALS1. In O-R, OsHistone is used as a reference gene. S, Zeatin content. T, Dihydrozeatin content. U, Gibberellin A4 content. V, Auxin content. The experimental setups were described as follows: CK (without Al and NaHS treatment), CK + NaHS (pretreated with 0.01 mmol/L NaHS), Al (treated with 70 mmol/L Al3+), and Al + NaHS (pretreated with 0.01 mmol/L NaHS and then treated with 70 mmol/L Al3+). Samples were taken on the 3rd and 5th days to measure each index. Values are Mean ± SD (n = 3). The different lowercase letters in each treatment day indicate significant difference at the 0.05 level by the Student-Newman-Keuls test.

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