RICE SCIENCE ›› 2007, Vol. 14 ›› Issue (1): 21-26 .

• Research Paper • Previous Articles     Next Articles

Source and Inheritance of the Within Cultivar Residual Variation
Detected in an indica Variety IR64

WU Jian-li 1,2, SHI Yong-feng 1, CHEN Jie 1, Casiana Vera CRUZ 2, ZHUANG Jie-yun 1, Hei LEUNG 2, ZHENG Kang-le 1   

  1. 1 Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 2 Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines
  • Received:2006-11-24 Online:2007-03-28 Published:2007-03-28
  • Contact: ZHENG Kang-le
  • Supported by:

    the Asia Rice Biotechnology Network, Chinese National Basic Research Project (Grant No. 2003DEB5J046) and Zhejiang Science and Technology Project (Grant No.2004C12032).

Abstract: The phenotypically uniform indica variety IR64 was chosen for study of the source and inheritance of within cultivar residual variation using a set of SSR markers. Residual heterogeneity in IR64 was identified on the short arm of chromosome 2 involving at least 5 SSR loci spanning nearly 30 cM. The SSR variations originated from the parental lines of IR64 (IR5657-33-2 / IR2061-465-1-5-5) and were segregating in the selfed bulk seed stock in a Mendelian manner for more than 20 years. This study verified that the within cultivar variations of SSR in a morphologically uniform variety IR64 of a selfing crop came from its parental lines, which has immediate and commercial applications including test of hybrid seed purity, varietal fingerprinting, and curation and propagation of germplasm collections.

Key words: simple sequence repeat, residual heterogeneity, within cultivar variation, rice