Mining Applicable Elite Alleles of Growing Duration, Plant Height and Panicle Number per Plant by Conditional QTL Mapping in Japonica Rice

Abstract

Abstract: Unconditional QTL mapping and conditional QTL mapping were conducted for growing duration (GD), plant height (PH) and effective panicle number per plant (PN) using a recombinant inbred line (RIL) population derived from a cross between Xiushui 79 and C Bao in japonica rice (Oryza sativa L.). The RIL population consisted of 254 lines was planted in two environments, Nanjing and Sihong, Jiangsu Province, China. Results showed that additive effects were major in all of QTLs for GD, PH and PN detected by the two methods, and the epistatic effects explained a small percent of phenotypic variation. No interactions were detected between additive QTL and environment, and between epistatic QTL pairs and environment. After growth duration was adjusted to an identical level, RM80-160bp was detected as an applicable elite allele for PN, with an additive effect of 0.71. After effective panicle number per plant was adjusted to an identical level, RM448-240bp was detected as an applicable elite allele for GD (shorten), with an additive effect of 4.64. After plant height was adjusted to an identical level, RM80-160bp was detected as an applicable elite allele for PN, with an additive effect of 0.62, and RM448-240bp was detected as an applicable elite allele for GD, with an additive effect of 3.89. These applicable elite alleles could be used to improve target traits without influencing the other two traits.

Key words: japonica rice, growth duration, plant height, effective panicle number per plant, unconditional QTL mapping, conditional QTL mapping, recombinant inbred line

JIANG Jian-hua, ZHAO Qi-bing, LIU Qiang-ming, CHEN Lan, CHEN Fu-long, QIAO Bao-jian, HONG De-lin. Mining Applicable Elite Alleles of Growing Duration, Plant Height and Panicle Number per Plant by Conditional QTL Mapping in Japonica Rice[J]. RICE SCIENCE.

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