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OsPIN1a Gene Participates in Regulating Negative Phototropism of Rice Roots

  1. 1College of Agriculture, Henan University of Science and Techenology, Luoyang 471003, China; 2College of Life Science, Shaoxing University, Shaoxing 312000, China; 3College of Life Science, Anhui Agricultural University, Hefei 230036, China; 4College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China
  • Online:2014-03-28 Published:2014-01-24
  • Contact: MO Yi-wei
  • Supported by:
    This work was supported by the grants from the National Natural Science Foundations of China (Grant Nos. 31071353 and 31100197), the Anhui Provincial Natural Science Fund of Youth, China (Grant No. 1308085QC50), and the Fund of Provincial Excellent Young Talents in Universities and Colleges, China (Grant No. 2012SQRL057).

Abstract: The complete open reading frame of OsPIN1a was amplified through reverse transcriptase- polymerase chain reaction (RT-PCR) based on the sequence deposited in GenBank to explore the relationship between the auxin efflux protein OsPIN1a and the negative phototropism of rice roots. Sequencing results showed that the GC content of OsPIN1a was 65.49%. The fusion expression vector pCAMBIA-1301-OsPIN1a::GFP containing the OsPIN1a gene and a coding green fluorescent protein (gfp) gene was constructed. The fusion vector was transferred into onion epidermal cells by Agrobacterium tumefaciens transformation. The transient expression of OsPIN1a-GFP was mainly located in the nucleus and cell membrane. Moreover, the transgenic plants were obtained by Agrobacterium-mediated genetic transformation. Molecular detection performed by using PCR and β-glucuronidase staining showed that the target construct was integrated into the genome of rice. The negative phototropic curvatures of the transgenic rice roots were higher than those of the wild type. Similarly, the expression levels of OsPIN1a in the transgenic plants were considerably higher than those in the wild-type plants. These results suggest that OsPIN1a is crucial in the negative phototropic curvature of rice roots.

Key words: rice , OsPIN1a, green fluorescent protein, transient expression, negative phototropism