Comparative Study on Growth Performance of Transgenic (Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity Regimes

doi:10.1016/S1672-6308(14)60301-2

Abstract

Abstract:

Transgenic Nipponbare which over-expressed a Na⁺/H⁺ antiporter gene OsNHX1 was used to compare its growth performance, water status and photosynthetic efficiency with its wild type under varying salinity regimes. Chlorophyll content, quantum yield and photosynthetic rate were measured to assess the impact of salinity stress on photosynthetic efficiency for transgenic and wild-type Nipponbare. Effects of salinity on water status and gas exchange to both lines were studied by measuring water use efficiency, instantaneous transpiration rate and stomatal conductance. Dry shoot weight and leaf area were determined after three months of growth to assess the impacts of salinity on the growth of those two lines. Our study showed that both lines were affected by salinity stress, however, the transgenic line showed higher photosynthetic efficiency, better utilization of water, and better growth due to low transpiration rate and stomatal conductance. Reduction of photosynthetic efficiency exhibited by the wild-type Nipponbare was correlated to its poor growth under salinity stress.

Key words: growth performance, salinity stress, Na+/H+ antiporter gene OsNHX1, transgenic rice, photosynthetic efficiency, water status

Kahrani Ishak Nurul, Sulaiman Zohrah, U. Tennakoon Kushan. Comparative Study on Growth Performance of Transgenic (Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity Regimes[J]. Rice Science, 2015, 22(6): 275-282.

Figures/Tables 5

Fig. 1. PCR amplification of OsNHX1 in transgenic and wild-type Nipponbare.（M, Marker; N1, Purified wild-type Nipponbare; N2, Unpurified wild-type Nipponbare.）

Fig. 2. Effect of salinity stress on maximal quantum yield of PSII (Photosystem II) for both transgenic and wild-type Nipponbare.（N, Wild-type Nipponbare; TN, Transgenic Nipponbare; 0, 50, 150 and 300 refer to 0, 50, 150 and 300 mmol/L salinity regimes, respectively.）

Fig. 3. Chlorophyll content meter of transgenic and wild-type Nipponbare under varying salinity regimes.（N, Wild-type Nipponbare; TN, Transgenic Nipponbare; 0, 50, 150 and 300 refer to 0, 50, 150 and 300 mmol/L salinity regimes, respectively.）

Fig. 4. Transpiration rate for both transgenic and wild-type Nipponbare under different salinity regimes.（N, Wild-type Nipponbare; TN, Transgenic Nipponbare; 0, 50, 150 and 300 refer to 0, 50, 150 and 300 mmol/L salinity regimes, respectively.）

Fig. 5. Shoot dry weight for both transgenic and wild-type Nipponbare under different salinity regimes.（N, Wild-type Nipponbare; TN, Transgenic Nipponbare; 0, 50, 150 and 300 refer to 0, 50, 150 and 300 mmol/L salinity regimes, respectively.）

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